COP1,PPS,OsCOP1

• Symbol: COP1,PPS,OsCOP1
• MSU: LOC_Os02g53140
• RAPdb: Os02g0771100

• Isolation and Molecular Characterization of the COP1 Gene Homolog from Rice,OryzasativaL. subsp.Indicavar. Pusa Basmati, 2001, DNA Res.
• The COP1 ortholog PPS regulates the juvenile-adult and vegetative-reproductive phase changes in rice, 2011, Plant Cell.
• Role of rice PPS in late vegetative and reproductive growth, 2012, Plant Signal Behav.
• Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1., 2017, Planta.
• OsCOP1 regulates embryo development and flavonoid biosynthesis in rice Oryza sativa L. , 2021, Theor Appl Genet.

Genbank accession number

• In spite of its prolonged juvenile phase, the pps-1 mutant flowers early, and this is associated with derepression of RAP1B expression in pps-1 plants independently of the Hd1-Hd3a/RFT1 photoperiodic pathway
• Its ability to regulate the vegetative phase change and the time of flowering suggests that rice PPS acquired novel functions during the evolution of rice/monocots
• The COP1 ortholog PPS regulates the juvenile-adult and vegetative-reproductive phase changes in rice
• Although the early vegetative phase and flowering time of pps-1 have been closely examined, the phenotypes in the late vegetative and reproductive phases are not yet well understood
• Role of rice PPS in late vegetative and reproductive growth
• Moreover, pps-1 had a small inflorescence meristem and small panicles
• These phenotypes indicate that in pps-1 the juvenile phase coexists with the late vegetative phase, resulting in small panicles
• Double mutant (d18-k pps-1) showed the same phenotype as the pps-1, indicating that PPS is upstream of GA biosynthetic genes
• In the ninth leaf blade of pps-1, the relative length of the midrib was comparable to the sixth leaf blade of wild-type
• The pps-1 mutant exhibits delayed expression of miR156 and miR172 and the suppression of GA biosynthetic genes, reducing the GA(3) content in this mutant
• The rice peter pan syndrome-1 (pps-1) mutant shows a prolonged juvenile phase and early flowering
• OsCOP1 regulates embryo development and flavonoid biosynthesis in rice (Oryza sativa L.)
• These results demonstrate that OsCOP1 is associated with embryo development and flavonoid biosynthesis in rice grains
• Novel mutations of OsCOP1 were identified to be responsible for yellowish pericarp and embryo lethal phenotype, which revealed that OsCOP1 plays a crucial role in flavonoid biosynthesis and embryogenesis in rice seed
• CRISPR/Cas9-targeted mutagenesis was used to knock out OsCOP1 by targeting its functional domains, and transgenic seed displayed the yel mutant phenotype
• Overexpression of OsCOP1 in a homozygous yel-hc mutant background restored pericarp color, and the aberrant flavonoid accumulation observed in yel-hc mutant was significantly reduced in the embryo and endosperm
• This study will facilitate a better understanding of the functional roles of OsCOP1 involved in early embryogenesis and flavonoid biosynthesis in rice seeds