| Categories genes  | Tags leaf  grain  tiller  grain yield  yield  cytokinin  breeding  tiller number  quality  tiller bud outgrowth  grain quality 
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  • Key message
    • Lower expression of OsAAP5 in the young leaf blade in indica varieties than in japonica varieties was associated with more tillers in indica than in japonica
    • The amino acid permease OsAAP5 regulates tiller number and grain yield in rice.
    • Downregulation of OsAAP5 expression in japonica using RNA interference (Ri) and CRISPR led to increases in tiller number and grain yield, while OsAAP5 overexpression (OE) had the opposite effect
    • Thus, OsAAP5 could regulate tiller bud outgrowth by affecting cytokinin levels, and knockout of OsAAP5 could be valuable for japonica breeding programs seeking high yield and grain quality
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