OsARF12

| Categories genes  | Tags primary root  root  phosphate  auxin  iron  homeostasis  mitochondria  transporter  transcription factor  pi 
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    • Here, we found a limiting factor of RSA–OsARF12–an auxin response factor whose knockout led to decreased primary root length in rice (Oryza sativa)
      • The root elongation zones of osarf12 and osarf12/25, which had lower auxin concentrations, were distinctly shorter than for the wild-type, possibly as a result of decreased expression of auxin synthesis genes OsYUCCAs and auxin efflux carriers OsPINs and OsPGPs
    • The knockout of OsARF12 also altered the abundance of mitochondrial iron-regulated (OsMIR), iron (Fe)-regulated transporter1 (OsIRT1) and short postembryonic root1 (OsSPR1) in roots of rice, and resulted in lower Fe content
      • The data provide evidence for the biological function of OsARF12, which is implicated in regulating root elongation
    • OsARF12, a transcription activator on auxin response gene, regulates root elongation and affects iron accumulation in rice (Oryza sativa)
    • Transcript analysis revealed that Pi-responsive genes–Phosphate starvation (OsIPS)1 and OsIPS2, SYG1/Pho81/XPR1(OsSPX1), Sulfoquinovosyldiacylglycerol 2 (OsSQD2), R2R3 MYB transcription factor (OsMYB2P-1) and Transport Inhibitor Response1 (OsTIR1)–were more abundant in the osarf12 and osarf12/25 mutants under +Pi/-Pi conditions
    • Auxin response factor (OsARF12), a novel regulator for phosphate homeostasis in rice (Oryza sativa)
    • Here, we report that an auxin response factor, OsARF12, functions in Pi homeostasis
    • Results from -Pi/1-naphthylphthalamic acid (NPA) treatments, and auxin reporter DR5::GUS staining suggest that root system alteration and Pi-induced auxin response were at least partially controlled by OsARF12
      • OsARF12 as a transcription activator can facilitate the expression of the auxin response element DR5::GFP, and OsARF12 was inhibited by osa-miRNA167d by transient expression in tobacco and rice callus
    • These findings enrich our understanding of the biological functions of OsARF12, which also acts in regulating Pi homeostasis
    • Measurement of element content, quantitative reverse transcription polymerase chain reaction analysis and acid phosphatases (APases) activity assay showed that the osarf12 mutant and osarf12/25 double mutant with P-intoxicated phenotypes had higher P concentrations, up-regulation of the Pi transporter encoding genes and increased APase activity under Pi-sufficient/-deficient (+Pi/-Pi, 0
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