OsHKT1;5,SKC1,OsHKT8,OsHK1;5

2015-01-20 | Categories genes  | Tags salinity stress  salinity  salt tolerance  homeostasis  xylem  leaf  shoot  transporter  salt stress  salt  vascular bundle  growth  tolerance  stress  reproductive  phloem  plasma membrane  development 

• Information
  • Symbol: OsHKT1;5,SKC1,OsHKT8,OsHK1;5
  • MSU: LOC_Os01g20160
  • RAPdb: Os01g0307500
• Publication
  • QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance, 2004, Theor Appl Genet.
  • Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice Oryza sativa L., 2012, BMC Plant Biol.
  • Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima: many sources, many genes, one mechanism?, 2013, BMC Plant Biol.
  • A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress, 2010, Plant Cell Environ.
  • A rice quantitative trait locus for salt tolerance encodes a sodium transporter, 2005, Nat Genet.
  • A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing, 2012, PLoS One.
  • OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice., 2017, Plant J.
  • Analysis of genetic diversity and population structure using SSR markers and validation of a Cleavage Amplified Polymorphic Sequences CAPS marker involving the sodium transporter OsHKT1;5 in saline tolerant rice Oryza sativa L. landraces., 2019, Gene.
  • HKT1;5 Transporter Gene Expression and Association of Amino Acid Substitutions With Salt Tolerance Across Rice Genotypes., 2019, Front Plant Sci.
  • Homology Modeling Identifies Crucial Amino-Acid Residues That Confer Higher Na+ Transport Capacity of OcHKT1;5 from Oryza coarctata Roxb , 2020, Plant Cell Physiol.
  • Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K + and Ca 2+ Acquisition and Homeostasis in Salinized Rice Roots , 2020, Int J Mol Sci.
  • CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, 2021, J Biotechnol.
  • Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis, 2020, EMBO J.
  • Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., 2022, J Integr Plant Biol.
  • The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., 2022, Int J Mol Sci.
• Genbank accession number
  • DQ148410
• Key message
  • Here we present a review on vital physiological functions of HKT transporters including AtHKT1;1 and OsHKT1;5 in preventing shoot Na(+) over-accumulation by mediating Na(+) exclusion from xylem vessels in the presence of a large amount of Na(+) thereby protecting leaves from salinity stress
  • We previously mapped a rice QTL, SKC1, that maintained K(+) homeostasis in the salt-tolerant variety under salt stress, consistent with the earlier finding that K(+) homeostasis is important in salt tolerance
  • Physiological analysis suggested that SKC1 is involved in regulating K(+)/Na(+) homeostasis under salt stress, providing a potential tool for improving salt tolerance in crops
  • SKC1 is preferentially expressed in the parenchyma cells surrounding the xylem vessels
  • The association of leaf Na+ concentrations with cultivar-groups was very weak, but association with the OsHKT1;5 allele was generally strong
  • Seven major and three minor alleles of OsHKT1;5 were identified, and their comparisons with the leaf Na+ concentration showed that the Aromatic allele conferred the highest exclusion and the Japonica allele the least
  • In addition, lower expression of OsHKT1;5 and OsSOS1 in old leaves may decrease frequency of retrieving Na+ from old leaf cells
  • To understand the molecular basis of this QTL, we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters
  • Voltage-clamp analysis showed that SKC1 protein functions as a Na(+)-selective transporter
  • For OsHKT1;5, both transcript abundance and protein structural features within the selectivity filter could control shoot Na(+) accumulation in a range of rice varieties
  • OsHKT1;5 mediates Na(+) exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice.
  • Additionally, direct introduction of (22) Na(+) tracer to leaf sheaths also demonstrated the involvement of OsHKT1;5 in xylem Na(+) unloading in leaf sheaths
  • Together with the characteristic (22) Na(+) allocation in the blade of the developing immature leaf in the mutants, these results suggest a novel function of OsHKT1;5 in mediating Na(+) exclusion in the phloem to prevent Na(+) transfer to young leaf blades
  • Our findings further demonstrate that the function of OsHKT1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress
  • Immuno-staining using an anti-OsHKT1;5 peptide antibody indicated that OsHKT1;5 is localized in cells adjacent to the xylem in roots
  • Furthermore, OsHKT1;5 was indicated to present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes
  • Salt tolerance QTL analysis of rice has revealed that the SKC1 locus, which is involved in a higher K(+) /Na(+) ratio in shoots, corresponds to the OsHKT1;5 gene encoding a Na(+) -selective transporter
  • However, physiological roles of OsHKT1;5 in rice exposed to salt stress remain elusive and no OsHKT1;5 gene disruption mutants have been characterized to date
  • Salt stress-induced increases in the OsHKT1;5 transcript was observed in roots and basal stems including basal nodes
  • We also report the development and validation of a new Cleavage Amplified Polymorphic Sequence (CAPS) marker (OsHKT1;5V395) that targets a codon in the sodium transporter gene OsHKT1;5 (Saltol/SKC1 locus) that is associated with sodium transport rates in the above rice landraces
  • Analysis of genetic diversity and population structure using SSR markers and validation of a Cleavage Amplified Polymorphic Sequences (CAPS) marker involving the sodium transporter OsHKT1;5 in saline tolerant rice (Oryza sativa L.) landraces.
  • Allelic variation in rice OsHKT1;5 sequence in specific landraces (Nona Bokra OsHKT1;5-NB/Nipponbare OsHKT1;5-Ni) correlates with variation in salt tolerance
  • In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance
  • Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K + and Ca 2+ Acquisition and Homeostasis in Salinized Rice Roots
  • Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • Using a previously generated high-throughput activation tagging-based T-DNA insertion mutant pool, we isolated a mutant exhibiting salt stress-sensitive phenotype, caused by a reduction in OsHKT1;5 transcripts
  • Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation.
  • The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5
• Connection
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHA1~OsSOS1, Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice Oryza sativa L., In addition, lower expression of OsHKT1;5 and OsSOS1 in old leaves may decrease frequency of retrieving Na+ from old leaf cells
  • OsHKT1;1~OsHKT4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress, Here we present a review on vital physiological functions of HKT transporters including AtHKT1;1 and OsHKT1;5 in preventing shoot Na(+) over-accumulation by mediating Na(+) exclusion from xylem vessels in the presence of a large amount of Na(+) thereby protecting leaves from salinity stress
  • OsHKT1;4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing, Amongst these transporters, the cereal HKT1;4 and HKT1;5 are responsible for Na(+) exclusion from photosynthetic tissues, a key mechanism for plant salinity tolerance
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsPEX11, OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa., Furthermore, qPCR data suggested that OsPEX11 acted as a positive regulator of salt tolerance by reinforcing the expression of several well-known rice transporters (OsHKT2;1, OsHKT1;5, OsLti6a, OsLti6b, OsSOS1, OsNHX1, and OsAKT1) involved in Na(+)/K(+) homeostasis in transgenic plants under salinity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsJRL, OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice., Overexpression of OsJRL in rice also enhanced salinity tolerance and increased the expression levels of a number of stress-related genes, including three LEA (late embryogenesis abundant proteins) genes (OsLEA19a, OsLEA23 and OsLEA24), three Na(+) transporter genes (OsHKT1;3, OsHKT1;4 and OsHKT1;5) and two DREB genes (OsDREB1A and OsDREB2B)
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Here, we show evidence that a rice Mg transporter OsMGT1 is required for the salt-tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na+ from the xylem sap at the root mature zone
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Taken together, our results suggest that Mg2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots
  • OsHAK1, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar., At the transcriptome level, the over-expression of OsHAK1 stimulated a number of stress-responsive genes as well as four genes known to be involved in Na+ homeostasis and the salinity response (OsHKT1;5, OsSOS1, OsLti6a and OsLti6b)
  • OsHKT1;4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Constitutive Contribution by the Rice OsHKT1;4 Na + Transporter to Xylem Sap Desalinization and Low Na + Accumulation in Young Leaves Under Low as High External Na + Conditions, Three rice HKT genes, OsHKT1;1, OsHKT1;4 and OsHKT1;5, are known to contribute to salt tolerance by reducing Na+ accumulation in shoots upon salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity
  • OsGrx_C7, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsHKT2;1~OsHKT1, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHA1~OsSOS1, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSDG721~SDG721, SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake, RNA sequencing (RNA-seq) analysis of wild-type Kitaake and ossdg721 mutants indicated that OsSDG721 positively regulates the expression level of HIGH-AFFINITY POTASSIUM (K+ ) TRANSPORTER1;5 (OsHKT1;5), which encodes a Na+ -selective transporter that maintains K+ /Na+ homeostasis under salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSDG721~SDG721, SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake, Furthermore, we showed that OsSDG721 binds to and deposits the H3K4me3 mark in the promoter and coding region of OsHKT1;5, thereby upregulating OsHKT1;5 expression under saline-alkaline stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNAC3, A NAC transcription factor OsNAC3 positively regulates ABA response and salt tolerance in rice., Furthermore, qRT-PCR and transcriptomic analysis were performed to identify the key OsNAC3 regulated genes related to ABA response and salt tolerance, such as OsHKT1;4, OsHKT1;5, OsLEA3-1, OsPM-1, OsPP2C68, and OsRAB-21
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • OsBAG4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsWRKY54, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsWRKY54, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., Furthermore, OsWRKY54 was found to regulate OsHKT1;5 expression by directly binding to the W-box motif in its promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHX4, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5

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