OsHT,OsLHT1

| Categories genes  | Tags transporter  leaf  root  growth  shoot  panicle  grain  sheath  grain yield  plasma membrane  amino acid transporter  root hair  nitrogen  vascular bundle  grain number  seed  starch  yield  vegetative  R protein  reproductive  quality  grain weight  flowering  panicle length 
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    • Using a degenerative probe designed according to the most conservative region of a known Lys- and His-specific amino acid transporter (LHT1) from Arabidopsis, we isolated a full-length cDNA named OsHT (histidine transporter of Oryza sativa L
    • Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters
    • Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae, suggesting that OsHT is a gene that encodes for a histidine transporter from rice
    • OsHT, a rice gene encoding for a plasma-membrane localized histidine transporter
    • QRT-PCR analysis indicated that OsLHT1 was expressed in various rice organs, including root, stem, flag leaf, flag leaf sheath and young panicle
    • Two oslht1 mutants were generated using CRISPR/Cas9 genome-editing technology, and the loss-of-function of OsLHT1 inhibited rice root and shoot growth, thereby markedly reducing grain yields
    • The aims of this study are to analyze the substrate selectivity of OsLHT1 and influence of its disruption on rice growth and fecundity
    • Our results indicated that OsLHT1 is an amino acid transporter with wide substrate specificity and with preference for neutral and acidic amino acids, and disruption of OsLHT1 function markedly inhibited rice growth and fecundity
    • OsLHT1 (Lysine/Histidine transporter) has been previously reported as a histidine transporter in yeast, but its substrate profile and function in planta are unclear
    • Transient expression in rice protoplast suggested OsLHT1 was localized to the plasma membrane, which is consistent with its function as an amino acid transporter
    • OsLHT1 is localized throughout the rice root, including root hairs, epidermis, cortex and stele, and to the leaf vasculature
    • Knockout of OsLHT1 in japonica resulted in reduced root uptake of amino acids
    • 15 N-ammonium uptake and subsequently the delivery of root synthesized amino acids to Oslht1 shoots were also significantly decreased, which was accompanied by reduced shoot growth
    • These results together provide evidence that OsLHT1 functions in both root uptake and root to shoot allocation of a broad spectrum of amino acids in rice
    • When expressed in yeast, OsLHT1 supported cell growth on a broad spectrum of amino acids and effectively transported aspartate, asparagine and glutamate
    • Rice OsLHT1 Functions in Leaf-to-Panicle Nitrogen Allocation for Grain Yield and Quality
    • The concentrations of both total N and free amino acids in the flag leaf were similar at anthesis between Oslht1 lines and WT while significantly higher in the mutants than WT at maturation
    • OsLHT1 is expressed in vascular bundles of leaves, rachis, and flowering organs
    • Oslht1 plants showed lower panicle length and seed setting rate, especially lower grain number per panicle and total grain weight
    • In this study, we planted knockout mutant lines of OsLHT1 together wild-type (WT) in paddy field for evaluating OsLHT1 function in N redistribution and grain production
    • Taken together, we conclude that OsLHT1 plays critical role in the translocation of amino acids from vegetative to reproductive organs for grain yield and quality of nutrition and functionality
    • The Oslht1 seeds contained higher proteins and most of the essential free amino acids, similar total starch but less amylose with lower paste viscosity than WT seeds
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