OsOPR7,OG1

2015-01-20 | Categories genes  | Tags sterility  jasmonic acid  ja  drought  jasmonic  map-based cloning  JA 

• Information
  • Symbol: OsOPR7,OG1
  • MSU: LOC_Os08g35740
  • RAPdb: Os08g0459600
• Publication
  • Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice, 2008, Planta.
  • OPEN GLUME1: a key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice., 2017, Plant Cell Rep.
• Genbank accession number
  • AK104843
• Key message
  • Furthermore, complementation analysis using an Arabidopsis opr3 mutant indicated that the OsOPR7 gene, but not OsOPR1, was able to complement the phenotypes of male sterility in the mutant caused by JA deficiency, and that JA production in the opr3 mutant was also restored by the expression of the OsOPR7 gene
  • Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice
  • Here, we provide evidence that an OPR family gene in rice chromosome 8, designated OsOPR7, encodes the enzyme involved in the JA biosynthesis
  • 5 h of mechanical wounding and drought stress, and the endogenous JA level started to increase in accordance with the increase in OsOPR7 expression
  • We conclude that the OsOPR7 gene encodes the enzyme catalysing the reduction of natural (+)-cis-OPDA for the JA biosynthesis in rice
  • Map-based cloning and subsequent complementation tests confirmed that OG1 encodes a peroxisome-localized 12-oxo-phytodienoic acid reductase-a key enzyme that reduces the precursor of JA
  • Loss-of-function of OG1 resulted in almost no JA accumulation
  • Exogenous JA treatment completely rescued the defects caused by the og1 mutation
• Connection
  • OsOPR7~OG1, OsPEX5, OsPEX5 regulates rice spikelet development through modulating jasmonic acid biosynthesis., • We show that OsPEX5 can physically interact with OsOPR7, an enzyme involved in jasmonic acid (JA) biosynthesis and is required for its import into peroxisome
  • OsOPR7~OG1, OsPEX5, OsPEX5 regulates rice spikelet development through modulating jasmonic acid biosynthesis., Similar to Ospex5 mutant, knockout mutant of OsOPR7 generated via the CRISPR-Cas9 technology has reduced levels of endogenous JA and also displays an abnormal spikelet phenotype
  • OsOPR7~OG1, OsPEX5, OsPEX5 regulates rice spikelet development through modulating jasmonic acid biosynthesis., Application of exogenous JA can partially rescue the abnormal spikelet phenotype of Ospex5 and Osopr7
  • OsOPR7~OG1, OsPEX5, OsPEX5 regulates rice spikelet development through modulating jasmonic acid biosynthesis., • Our results suggest that OsPEX5 plays a critical role in regulating spikelet development through mediating peroxisomal import of OsOPR7, thus providing new insights into regulation of JA biosynthesis in plants and expanding our understanding of the biological role of JA in regulating rice reproduction

Prev     Next