OsPUB73

• Symbol: OsPUB73
• MSU: LOC_Os02g28870
• RAPdb: Os02g0490000

• Cytological and transcriptome analyses reveal OsPUB73 defect affects the gene expression associated with tapetum or pollen exine abnormality in rice., 2019, BMC Plant Biol.

Genbank accession number

• Cytological and transcriptome analyses reveal OsPUB73 defect affects the gene expression associated with tapetum or pollen exine abnormality in rice.
• In the present study, we knocked out OsPUB73 by using the CRISPR/Cas9 system and studied the cytological and transcriptome of the gene-defect associated with pollen development and sterility in the rice variety (Taichung 65)
• The ospub73 displayed low pollen fertility (19
• Cytological observation showed tapetum vacuolated at the meiosis stage and pollen exine was abnormal at the bi-cellular pollen stage of ospub73
• These results suggested OsPUB73 may play an important role in tapetal or pollen exine development and resulted in pollen partial sterility
• The expression pattern of OsPUB73 showed that it was highly expressed in the anther during meiosis stage
• RNA-seq analysis detected 2240 down and 571 up-regulated genes in anther of ospub73 compared with WT during meiosis stage
• Our results revealed that OsPUB73 plays an important role in rice male reproductive development, which provides valuable information about the molecular mechanisms of the U-box in rice male reproductive development