Pho1

2015-01-20 | Categories genes  | Tags starch  starch biosynthesis  temperature  seed  endosperm  growth  shoot  homeostasis  plant growth  Pi  pi  Pi homeostasis 

• Information
  • Symbol: Pho1
  • MSU: LOC_Os03g55090
  • RAPdb: Os03g0758100
• Publication
  • Mutation of the plastidial alpha-glucan phosphorylase gene in rice affects the synthesis and structure of starch in the endosperm, 2008, Plant Cell.
  • Rice endosperm starch phosphorylase Pho1 assembles with disproportionating enzyme Dpe1 to form a protein complex that enhances synthesis of malto-oligosaccharides., 2016, J Biol Chem.
  • Modulation of Shoot Phosphate Level and Growth by PHOSPHATE1 Upstream Open Reading Frame , 2020, Plant Physiol.
• Genbank accession number
  • AB441692
• Key message
  • The loss of Pho1 caused smaller starch granules to accumulate and modified the amylopectin structure
  • These results strongly suggest that Pho1 plays a crucial role in starch biosynthesis in rice endosperm at low temperatures and that one or more other factors can complement the function of Pho1 at high temperatures
  • The phenotype of the pho1 mutation was temperature dependent
  • Plastidial phosphorylase (Pho1) accounts for approximately 96% of the total phosphorylase activity in developing rice (Oryza sativa) seeds
  • Variation in the morphological and biochemical phenotype of individual seeds was common to all 15 pho1-independent homozygous mutant lines studied, indicating that this phenotype was caused solely by the genetic defect
  • Our work suggests that regulation of PHO1 expression via its uORF might be a genetic resource useful-both in natural populations and in the context of genome editing-toward improving plant growth under Pi-deficient conditions
  • We further show that natural accessions lacking the PHO1 uORF exhibit higher PHO1 protein levels and shoot Pi content
  • Increased shoot Pi content was linked to the absence of the PHO1 uORF in a population of F2 segregants
  • In this work, we demonstrate that an upstream open reading frame (uORF) present in the 5’ untranslated region of the Arabidopsis (Arabidopsis thaliana) PHO1 inhibits its translation and influences Pi homeostasis
  • A point mutation removing the PHO1 uORF (uORF) in transgenic Arabidopsis resulted in increased association of its mRNA with polysomes and led to higher PHO1 protein levels, independent of Pi availability
• Connection
  • DPE1, Pho1, Rice endosperm starch phosphorylase Pho1 assembles with disproportionating enzyme Dpe1 to form a protein complex that enhances synthesis of malto-oligosaccharides., Rice endosperm starch phosphorylase (Pho1) assembles with disproportionating enzyme (Dpe1) to form a protein complex that enhances synthesis of malto-oligosaccharides.
  • OsPHO1;2, Pho1, Node-localized transporters of phosphorus essential for seed development in rice., Here, we found that two members (OsPHO1;1 and OsPHO1;2) belonging to PHO1 gene family, are involved in the distribution of P to the seeds in rice
  • OsPHO1;1, Pho1, Node-localized transporters of phosphorus essential for seed development in rice., Here, we found that two members (OsPHO1;1 and OsPHO1;2) belonging to PHO1 gene family, are involved in the distribution of P to the seeds in rice

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