funRiceGenes 2024-03-30T17:05:55+00:00 venyao@qq.com ZFP252,RZF71 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os12g39400
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    • We previously identified a salt and drought stress-responsive TFIIIA-type zinc finger protein gene ZFP252 from rice
    • We found that overexpression of ZFP252 in rice increased the amount of free proline and soluble sugars, elevated the expression of stress defense genes and enhanced rice tolerance to salt and drought stresses, as compared with ZFP252 antisense and non-transgenic plants
    • Our findings suggest that ZFP252 plays an important role in rice response to salt and drought stresses and is useful in engineering crop plants with enhanced tolerance to salt and drought stresses
    • Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.)
    • These results indicated that the RZF71 may play an important role in rice responses to salt and osmotic stresses as a transcription factor
    • The semi-quantitative RT-PCR assay showed RZF71 was strongly induced by high-salinity and 20% PEG6000 treatments, but not regulated by low temperature and ABA (abscisic acid) treatments
    • The expression profiling showed that RZF71 was constitutively expressed in roots, culms, leaves, and flowering spikes
    • A rice zinc-finger protein gene, RZF71, encoding the C2H2-type zinc-finger transcription factor was isolated from rice
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    • ]]>         ZOS8-11 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os08g40560
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    • ]]>         ZL16 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os08g12840
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    • ZL16 was ubiquitously expressed in various plant organs, with a pronounced level in the young leaf
    • The zl16 mutant showed chlorotic abnormalities in the transverse sectors of the young leaves of seedlings
    • The use of transmission electron microscopy (TEM) demonstrated that dramatic defects occurred in variegated zl16 leaves during the early development of a chloroplast
    • A subcellular localization experiment indicated that ZL16 was targeted in the chloroplast
    • Furthermore, we analyzed the expression of some nuclear genes involved in chloroplast development, and found they were altered in the zl16 mutant
    • Map-based cloning revealed that ZL16 encodes a -hydroxyacyl-ACP dehydratase (HAD) involved in de novo fatty acid synthesis
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    • ]]>         ZFP245 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os07g39970
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    • Overproduction of ZFP245 enhanced the activities of reactive oxygen species-scavenging enzymes under stress conditions and increased the tolerance of rice seedlings to oxidative stress
    • However, ZFP245 was not regulated by high salt or abscisic acid treatment
    • The semi-quantitative-RT-PCR assay revealed ZFP245 was strongly induced after 6 h exposure to cold and drought stresses, and then reduced to the baseline
    • Taken together, ZFP245, as the first identified C2H2-type zinc finger protein involved in stress response in monocots probably plays a role as a transcription regulator in plant cold and drought responses through an ABA-independent pathway
    • A C2H2-type zinc finger protein gene ZFP245 was cloned by RT-PCR approach from cold treated rice seedlings
    • Our data suggest that ZFP245 may contribute to the tolerance of rice plants to cold and drought stresses by regulating proline levels and reactive oxygen species-scavenging activities, and therefore may be useful for developing transgenic crops with enhanced tolerance to abiotic stress
    • ZFP245 is a cold- and drought-responsive gene that encodes a zinc finger protein in rice
    • Transgenic rice plants overexpressing ZFP245 were generated and found to display high tolerance to cold and drought stresses
    • Increased tolerance of rice to cold, drought and oxidative stresses mediated by the overexpression of a gene that encodes the zinc finger protein ZFP245
    • Tissue expression analysis showed that ZFP245 was constitutively expressed in various rice tissues including roots, stems, leaves and spikes
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    • ]]>         ZFP177 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os07g07350
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    • These results suggested that ZFP177 might play crucial but differential roles in plant responses to various abiotic stresses
    • Through microarray analysis, it was found that four genes (ZFP177, ZFP181, ZFP176, ZFP173), two genes (ZFP181 and ZFP176) and one gene (ZFP157) were significantly induced by cold, drought and H(2)O(2) treatments, respectively
    • Overexpression of ZFP177 in tobacco conferred tolerance of transgenic plants to both low and high temperature stresses, but increased sensitivity to salt and drought stresses
    • Further expression analysis showed that ZFP177 was responsive to both cold and heat stresses, but down-regulated by salt
    • Expression analysis of rice A20/AN1-type zinc finger genes and characterization of ZFP177 that contributes to temperature stress tolerance
    • The subcellular localization assay indicated that ZFP177 was localized in cytoplasm in tobacco leaf and root cells
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    • ]]>         ZN 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os06g02580
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    • Virus-induced gene silencing of a ZN homolog in Nicotiana benthamiana causes leaf variegation with sporadic green/yellow sectors, indicating that ZN is essential for chloroplast biogenesis during early leaf development
    • Together, we propose that ZN is required for protecting developing chloroplasts, especially during the assembly of thylakoid protein complexes, from incidental light after darkness
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    • ]]>         Z15 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os05g12680
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    • The expression of Z15 is induced by moderate low temperature (18<U+2009>C)
    • The mutation of Z15 influenced the expression of two downstream genes, OsWRKY71 and OsMYB4, that were responsive to moderate low temperature
    • The results show that Z15 plays a crucial role in the early stages of the response to moderate low temperature in rice
    • Further study indiceted that Z15 plays a crucial role in the early stages of the response to moderate low temperature in rice
    • Subcellular localization analysis indicates that Z15 and z15 are localized on the plasma membrane
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    • ]]>         ZEP1,OsZEP 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os04g37960
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    • In the absence of OsAM1, many other critical meiotic components, including PAIR2, ZEP1 and OsMER3, could not be correctly installed onto chromosomes
    • The expression levels of the drought-responsive genes RD22, SODA1, bZIP46 and POD, as well as the ABA synthetic gene ZEP1 were up-regulated in the OsDIL-overexpression lines but the ABA degradation gene ABAOX3 was down-regulated
    • In addition, ZEP1 is reloaded onto chromosomes in early microspores as the chromosome decondense, suggesting that ZEP1 might have other biological functions during this process
    • The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice
    • Finally, we found that the centromeric localization of OsSGO1 depends on OsAM1, not other meiotic proteins such as OsREC8, PAIR2, OsMER3, or ZEP1
    • Several key meiotic proteins, including ZEP1 and OsMER3, were not loaded normally onto chromosomes in Oscom1 mutants, whereas the localization of OsREC8, PAIR2 and PAIR3 seemed to be normal
    • Moreover, OsCOM1 was loaded normally onto meiotic chromosomes in Osrec8, zep1 and Osmer3 mutants, but could not be properly loaded in Osam1, pair2 and OsSPO11-1(RNAi) plants
    • In contrast, in pair2, Osmer3 and zep1 mutants, OsAM1 could be loaded normally, suggesting that OsAM1 plays a fundamental role in building the proper chromosome structure at the beginning of meiosis
    • Carotenoids enter the xanthophyll cycle, and the metabolites that differentially accumulate in the striped leaves include zeaxanthin and its derivatives for photooxidative stress protection, driven by the upregulated expression of OsZEP
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    • ]]>         ZFP15 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os03g60570
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    • Rice ZFP15 gene encoding for a novel C2H2-type zinc finger protein lacking DLN box, is regulated by spike development but not by abiotic stresses
    • Besides, ZFP15 was shown to accumulate much more in flowering spike than in immature spike
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    • ]]>         ZFP182,ZOS3-21 2015-01-20T00:00:00+00:00 Wen Yao (姚文) https://funricegenes.github.io//LOC_Os03g60560
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    • Overexpression of ZFP182 significantly enhanced multiple abiotic stress tolerances, including salt, cold and drought tolerances in transgenic rice
    • The expression analysis showed that ZFP182 gene was constitutively expressed in leaves, culms, roots and spikes at the adult rice plants, and markedly induced in the seedlings by cold (4 degrees C), 150 mM NaCl and 0
    • Nipponbare) C(2) H(2) -type ZFP ZFP182 in ABA-induced antioxidant defense and the relationship between ZFP182 and two rice MAPKs, OsMPK1 and OsMPK5 in ABA signaling were investigated
    • These results indicate that ZFP182 is required for ABA-induced antioxidant defense and the expression of ZFP182 is regulated by rice MAPKs in ABA signaling
    • The C2H2-type zinc finger protein ZFP182 is involved in abscisic acid-induced antioxidant defense in rice
    • Expression of ZFP182 in transgenic tobacco and overexpression in rice increased plant tolerance to salt stress
    • These results demonstrated that ZFP182 might be involved in plant responses to salt stress
    • ABA treatment induced the increases in the expression of ZFP182, OsMPK1 and OsMPK5, and the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) in rice leaves
    • Besides, OsMPK1 and OsMPK5 were shown to be required for the up-regulation in the expression of ZFP182 in ABA signaling, but ZFP182 did not mediate the ABA-induced up-regulation in the expression of OsMPK1 and OsMPK5
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