OsNAC016

• Symbol: OsNAC016
• MSU: LOC_Os01g01430
• RAPdb: Os01g0104200

• OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., 2022, Plant Physiol.

Genbank accession number

• Further investigation revealed that OsNAC016 regulated the expression of the brassinosteroid biosynthesis gene D2 by binding to its promoter
• Moreover, OsNAC016 interacted with and was phosphorylated by GSK3/SHAGGY-LIKE KINASE2 (GSK2), a negative regulator in the BR pathway
• OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8.
• Meanwhile, the mutant osnac016 had improved drought stress tolerance, supported by a decreased water loss rate and enhanced stomatal closure in response to exogenous ABA, but OsNAC016-overexpressing plants showed attenuated drought tolerance and reduced ABA sensitivity
• Our findings establish that OsNAC016 is positively involved in BR-regulated rice architecture, negatively modulates ABA-mediated drought tolerance, and is regulated by GSK2, SAPK8, and OsPUB43 through post-translational modification
• Further, OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE8 (SAPK8) phosphorylated OsNAC016 and reduced its stability
• The loss-of-function mutant osnac016 exhibited erect leaves and shortened internodes, but OsNAC016-overexpressing plants had opposite phenotypes

• D2~CYP90D2~SMG11~OsD2, OsNAC016, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., Further investigation revealed that OsNAC016 regulated the expression of the brassinosteroid biosynthesis gene D2 by binding to its promoter
• GSK2~OsGSK2~OsSK22, OsNAC016, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8.
• GSK2~OsGSK2~OsSK22, OsNAC016, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., Moreover, OsNAC016 interacted with and was phosphorylated by GSK3/SHAGGY-LIKE KINASE2 (GSK2), a negative regulator in the BR pathway
• GSK2~OsGSK2~OsSK22, OsNAC016, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., Our findings establish that OsNAC016 is positively involved in BR-regulated rice architecture, negatively modulates ABA-mediated drought tolerance, and is regulated by GSK2, SAPK8, and OsPUB43 through post-translational modification
• OsNAC016, OsSAPK8~SAPK8, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8.
• OsNAC016, OsSAPK8~SAPK8, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., Further, OSMOTIC STRESS/ABA-ACTIVATED PROTEIN KINASE8 (SAPK8) phosphorylated OsNAC016 and reduced its stability
• OsNAC016, OsSAPK8~SAPK8, OsNAC016 regulates plant architecture and drought tolerance by interacting with the kinases GSK2 and SAPK8., Our findings establish that OsNAC016 is positively involved in BR-regulated rice architecture, negatively modulates ABA-mediated drought tolerance, and is regulated by GSK2, SAPK8, and OsPUB43 through post-translational modification
• OsNAC016, OsSPX2, Transcription factor OsNAC016 negatively regulates phosphate-starvation response in rice., Chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) analysis and transient transactivation assays indicated that OsNAC016 could activate the SPX-domain-containing protein 2 (OsSPX2) gene through binding to its promoter
• OsNAC016, OsSPX2, Transcription factor OsNAC016 negatively regulates phosphate-starvation response in rice., Further, we found that Pi starvation enhanced OsNAC016 binding to the OsSPX2 promoter, thus strongly promoting OsSPX2 expression
• OsNAC016, OsSPX2, Transcription factor OsNAC016 negatively regulates phosphate-starvation response in rice., Moreover, similar to OsSPX2, OsNAC016 negatively regulates leaf inclination by repressing the cell elongation in lamina joint in rice under Pi-starvation stress
• OsNAC016, OsSPX2, Transcription factor OsNAC016 negatively regulates phosphate-starvation response in rice., Together, our findings demonstrate that OsNAC016 negatively regulates rice phosphate-starvation response and leaf inclination by activating OsSPX2 expression under Pi-starvation conditions