- Information
- Symbol: OsbHLH035,OsbHLH35
- MSU: LOC_Os01g06640
- RAPdb: Os01g0159800
- Publication
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Genbank accession number
- Key message
- The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively.
- The bHLH TF OsbHLH035 is a salt-induced gene that is primarily expressed in germinating seeds and seedlings
- Moreover, Osbhlh035 mutant seedlings are unable to recover from salt-stress treatment
- Consistently, sodium is over-accumulated in aerial tissues but slightly reduced in terrestrial tissues from Osbhlh035 seedlings after salt treatment
- Furthermore, genetic complementation can restore both the delayed seed germination and the impaired recovery of salt-treated Osbhlh035 seedlings to normal growth
- OsbHLH035 mediates seed germination and seedling recovery after salt stress relief through the ABA-dependent and ABA-independent activation of OsHKT pathways, respectively
- Osbhlh035 mutants show delayed seed germination, particularly under salt-stress conditions
- The expression of OsbHLH035 is induced by drought and salinity
- In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating Osbhlh035 mutant seeds is downregulated
- Stable expression of GFP-fused OsbHLH035 in rice transgenic plants revealed that this protein is predominantly localized to the nucleus
- Tightly controlled expression of OsbHLH35 is critical for anther development in rice
- Our data suggest that OsbHLH35 plays an essential role in anther development in rice and the fine control of its expression is crucial to ensure proper seed production
- Rice transgenic plants expressing GUS reporter gene under the control of OsbHLH35 promoter (pOsbHLH35::GUS) showed that this TF specifically accumulates in anthers at the meiosis stage and in other spikelet tissues
- Plants overexpressing OsbHLH35 presented small and curved anthers, leading to a reduction of 72 % on seed production
- Yeast one-hybrid screening identified three members of the Growth-Regulating Factor (GRF) family, OsGRF3, OsGRF4, and OsGRF11, as transcriptional regulators of OsbHLH35
- Connection
- OsABA2, OsbHLH035~OsbHLH35, The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively., In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating Osbhlh035 mutant seeds is downregulated
- OsABA8ox1, OsbHLH035~OsbHLH35, The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively., In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating Osbhlh035 mutant seeds is downregulated
- OsbHLH035~OsbHLH35, OsHKT1;3, The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively., Additionally, the expression of the sodium transporters OsHKT1;3 and 1;5 is reduced in Osbhlh035 aerial and terrestrial tissues, respectively
- GS2~OsGRF4~GL2~GLW2~LGS1, OsbHLH035~OsbHLH35, Tightly controlled expression of OsbHLH35 is critical for anther development in rice, Yeast one-hybrid screening identified three members of the Growth-Regulating Factor (GRF) family, OsGRF3, OsGRF4, and OsGRF11, as transcriptional regulators of OsbHLH35
- OsbHLH035~OsbHLH35, OsGRF11, Tightly controlled expression of OsbHLH35 is critical for anther development in rice, Yeast one-hybrid screening identified three members of the Growth-Regulating Factor (GRF) family, OsGRF3, OsGRF4, and OsGRF11, as transcriptional regulators of OsbHLH35
- OsbHLH035~OsbHLH35, OsGRF11, Tightly controlled expression of OsbHLH35 is critical for anther development in rice, Transactivation assay showed that OsGRF11 negatively regulates OsbHLH35 expression in Arabidopsis protoplasts
- OsbHLH035~OsbHLH35, OsGRF11, Tightly controlled expression of OsbHLH35 is critical for anther development in rice, This regulation was also observed in planta through the analysis of transgenic plants overexpressing OsGRF11 (OsGRF11OE), confirming that OsGRF11 is a negative regulator of OsbHLH35 in rice
- OsbHLH035~OsbHLH35, OsGRF3, Tightly controlled expression of OsbHLH35 is critical for anther development in rice, Yeast one-hybrid screening identified three members of the Growth-Regulating Factor (GRF) family, OsGRF3, OsGRF4, and OsGRF11, as transcriptional regulators of OsbHLH35
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