OsRIP1,OsjRIP1.1

• Symbol: OsRIP1,OsjRIP1.1
• MSU: LOC_Os01g06740
• RAPdb: Os01g0160800

• Involvement of OsRIP1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens., 2019, Phytochemistry.
• Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice, 2021, Int J Mol Sci.
• The type-1 ribosome inactivating protein OsRIP1 triggers caspase-independent apoptotic-like death in HeLa cells., 2021, Food Chem Toxicol.
• Differential effects of the recombinant type 1 ribosome-inactivating protein, OsRIP1, on growth of PSB-D and BY-2 cells., 2022, Front Plant Sci.

Genbank accession number

• The data obtained in this research indicate that OsRIP1 can play a role in plant defense against herbivorous insects
• OsRIP1 inactivated insect ribosomes in vitro, suggesting that its toxicity relates to the enzymatic activity of OsRIP1
• Furthermore, recombinant OsRIP1 was toxic for brown planthoppers when administered through liquid artificial diet
• Over-expression of OsRIP1 in transgenic rice plants did not affect the performance of insects reared on these plants, most likely due to insufficient concentrations of OsRIP1 in the phloem
• OsRIP1 provoked HeLa cells to undergo caspase-independent cell death, associated with a significant transcriptional upregulation of the apoptotic gene PUMA, interferon regulatory factor 1 (IRF1) and the autophagy-related marker LC3
• Human cervical cancer HeLa cells were incubated in the presence of OsRIP1 for 24-72 h OsRIP1 treatment yielded an anti-proliferation response of the HeLa cells and resulted in apoptotic-like blebbing of the plasma membrane without causing DNA fragmentation
• Our study provides the first evidence that OsRIP1 exerts differential effects on the growth of PSB-D and BY-2 cells
• OsRIP1 triggered cell death in tobacco BY-2 cells but not in Arabidopsis PSB-D cells
• Targeting of exogenous OsRIP1 to plant vacuoles and OsRIP1-induced accumulation of transcripts for vacuolar processing enzymes (VPEs) indicated that OsRIP1 provoked plant cell death in tobacco BY-2 cells through the activation of VPEs and subsequent vacuolar disruption, which was probably independent of its N-glycosylase activity on cytosolic ribosomes
• The vacuole-dependent cell death pathway is associated with the lethal effect of the exogenously applied OsRIP1 on BY-2 cells

• OsbZIP01~OsRE1, OsRIP1~OsjRIP1.1, OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression, OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression
• OsbZIP01~OsRE1, OsRIP1~OsjRIP1.1, OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression, Our genetic data showed that OsRE1 and OsRIP1 may function upstream of Ehd1 in regulating heading date
• OsbZIP01~OsRE1, OsRIP1~OsjRIP1.1, OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression, Together, our results suggest that OsRE1 functions cooperatively with OsRIP1 to regulate heading date through finely modulating the expression of Ehd1
• OsbZIP01~OsRE1, OsRIP1~OsjRIP1.1, OsRE1 interacts with OsRIP1 to regulate rice heading date by finely modulating Ehd1 expression, In addition, OsRE1 and OsRIP1 are two minor heading date regulators, which are more desirable for fine-tuning heading date to improve rice regional adaptability
• nuRIP~OsjRIP11.2, OsRIP1~OsjRIP1.1, Effect of RIP Overexpression on Abiotic Stress Tolerance and Development of Rice, This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP
• LC3, OsRIP1~OsjRIP1.1, The type-1 ribosome inactivating protein OsRIP1 triggers caspase-independent apoptotic-like death in HeLa cells., OsRIP1 provoked HeLa cells to undergo caspase-independent cell death, associated with a significant transcriptional upregulation of the apoptotic gene PUMA, interferon regulatory factor 1 (IRF1) and the autophagy-related marker LC3