- Information
- Symbol: ROS1a,OsROS1a
- MSU: LOC_Os01g11900
- RAPdb: Os01g0217900
- Publication
- A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny, 2012, Plant J.
- DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm., 2019, Proc Natl Acad Sci U S A.
- Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice Oryza sativa L.., 2022, Int J Mol Sci.
- Genbank accession number
- Key message
- These T(0) plants exhibited no overt morphological phenotypes during the vegetative phase, and GUS staining showed ROS1a expression in pollen, unfertilized ovules and meristematic cells
- Even in the presence of the wild-type paternal ROS1a allele, the maternal ros1a-GUS1 allele caused failure of early-stage endosperm development, resulting in incomplete embryo development, with embryogenesis producing irregular but viable embryos that failed to complete seed dormancy, implying non-equivalent maternal and paternal contribution of ROS1a in endosperm development
- DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm.
- Here, we report genome-wide analyses of DNA methylation in wild-type and ros1a mutant sperm and vegetative cells
- We find that the rice vegetative cell genome is locally hypomethylated compared with sperm by a process that requires ROS1a activity
- We show that many ROS1a target sequences in the vegetative cell are hypomethylated in the rice central cell, suggesting that ROS1a also demethylates the central cell genome
- Similarly, the expression of genes involved in pollen and anther development was decreased in osros1a mutants as compared to WT
- The osros1a mutants had shrink spikelets, smaller anthers and pollen grains, and were not stained by iodine staining showing a significant reduction in total soluble sugar and starch contents as compared to wildtype (WT), which caused complete male sterility
- Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice (Oryza sativa L.).
- Combined with previous studies, our findings suggested that the RRMF domain in OsROS1a is the functional domain and loss of RRMF for OsROS1a causes sterility in rice
- Connection
- OsPKS2, ROS1a~OsROS1a, Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice Oryza sativa L.., Furthermore, bisulfite sequencing showed that the CG and CHG methylation of the OsPKS2 gene promoter was significantly increased in the osros1a mutant, which caused a reduced expression of OsPKS2 in osros1a mutants
- ROS1a~OsROS1a, TDR, Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice Oryza sativa L.., DNA methylation of the TDR gene promoter was similar between WT and osros1a mutants, indicating that the DNA methylation effect by OsROS1a was gene specific
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