| Categories genes  | Tags meristem  vegetative  dormancy  seed  pollen  endosperm  development  anther  sterility  starch  sugar  anther development  male sterility 
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    • These T(0) plants exhibited no overt morphological phenotypes during the vegetative phase, and GUS staining showed ROS1a expression in pollen, unfertilized ovules and meristematic cells
    • Even in the presence of the wild-type paternal ROS1a allele, the maternal ros1a-GUS1 allele caused failure of early-stage endosperm development, resulting in incomplete embryo development, with embryogenesis producing irregular but viable embryos that failed to complete seed dormancy, implying non-equivalent maternal and paternal contribution of ROS1a in endosperm development
    • DNA demethylation by ROS1a in rice vegetative cells promotes methylation in sperm.
    • Here, we report genome-wide analyses of DNA methylation in wild-type and ros1a mutant sperm and vegetative cells
    • We find that the rice vegetative cell genome is locally hypomethylated compared with sperm by a process that requires ROS1a activity
    • We show that many ROS1a target sequences in the vegetative cell are hypomethylated in the rice central cell, suggesting that ROS1a also demethylates the central cell genome
    • Similarly, the expression of genes involved in pollen and anther development was decreased in osros1a mutants as compared to WT
    • The osros1a mutants had shrink spikelets, smaller anthers and pollen grains, and were not stained by iodine staining showing a significant reduction in total soluble sugar and starch contents as compared to wildtype (WT), which caused complete male sterility
    • Loss of Function of the RRMF Domain in OsROS1a Causes Sterility in Rice (Oryza sativa L.).
    • Combined with previous studies, our findings suggested that the RRMF domain in OsROS1a is the functional domain and loss of RRMF for OsROS1a causes sterility in rice
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