OsHKT1;5,SKC1,OsHKT8,OsHK1;5

2015-01-20 | Categories genes  | Tags salinity stress  salinity  salt tolerance  homeostasis  xylem  leaf  shoot  transporter  salt stress  salt  vascular bundle  growth  tolerance  stress  reproductive  phloem  plasma membrane  development 

• Information
  • Symbol: OsHKT1;5,SKC1,OsHKT8,OsHK1;5
  • MSU: LOC_Os01g20160
  • RAPdb: Os01g0307500
• Publication
  • QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance, 2004, Theor Appl Genet.
  • Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice Oryza sativa L., 2012, BMC Plant Biol.
  • Salinity tolerance, Na+ exclusion and allele mining of HKT1;5 in Oryza sativa and O. glaberrima: many sources, many genes, one mechanism?, 2013, BMC Plant Biol.
  • A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress, 2010, Plant Cell Environ.
  • A rice quantitative trait locus for salt tolerance encodes a sodium transporter, 2005, Nat Genet.
  • A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing, 2012, PLoS One.
  • OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice., 2017, Plant J.
  • Analysis of genetic diversity and population structure using SSR markers and validation of a Cleavage Amplified Polymorphic Sequences CAPS marker involving the sodium transporter OsHKT1;5 in saline tolerant rice Oryza sativa L. landraces., 2019, Gene.
  • HKT1;5 Transporter Gene Expression and Association of Amino Acid Substitutions With Salt Tolerance Across Rice Genotypes., 2019, Front Plant Sci.
  • Homology Modeling Identifies Crucial Amino-Acid Residues That Confer Higher Na+ Transport Capacity of OcHKT1;5 from Oryza coarctata Roxb , 2020, Plant Cell Physiol.
  • Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K + and Ca 2+ Acquisition and Homeostasis in Salinized Rice Roots , 2020, Int J Mol Sci.
  • CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, 2021, J Biotechnol.
  • Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1-mediated sodium homeostasis, 2020, EMBO J.
  • Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., 2022, J Integr Plant Biol.
  • The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., 2022, Int J Mol Sci.
  • Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., 2023, Front Plant Sci.
• Genbank accession number
  • DQ148410
• Key message
  • Here we present a review on vital physiological functions of HKT transporters including AtHKT1;1 and OsHKT1;5 in preventing shoot Na(+) over-accumulation by mediating Na(+) exclusion from xylem vessels in the presence of a large amount of Na(+) thereby protecting leaves from salinity stress
  • We previously mapped a rice QTL, SKC1, that maintained K(+) homeostasis in the salt-tolerant variety under salt stress, consistent with the earlier finding that K(+) homeostasis is important in salt tolerance
  • Physiological analysis suggested that SKC1 is involved in regulating K(+)/Na(+) homeostasis under salt stress, providing a potential tool for improving salt tolerance in crops
  • SKC1 is preferentially expressed in the parenchyma cells surrounding the xylem vessels
  • The association of leaf Na+ concentrations with cultivar-groups was very weak, but association with the OsHKT1;5 allele was generally strong
  • Seven major and three minor alleles of OsHKT1;5 were identified, and their comparisons with the leaf Na+ concentration showed that the Aromatic allele conferred the highest exclusion and the Japonica allele the least
  • In addition, lower expression of OsHKT1;5 and OsSOS1 in old leaves may decrease frequency of retrieving Na+ from old leaf cells
  • To understand the molecular basis of this QTL, we isolated the SKC1 gene by map-based cloning and found that it encoded a member of HKT-type transporters
  • Voltage-clamp analysis showed that SKC1 protein functions as a Na(+)-selective transporter
  • For OsHKT1;5, both transcript abundance and protein structural features within the selectivity filter could control shoot Na(+) accumulation in a range of rice varieties
  • OsHKT1;5 mediates Na(+) exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice.
  • Additionally, direct introduction of (22) Na(+) tracer to leaf sheaths also demonstrated the involvement of OsHKT1;5 in xylem Na(+) unloading in leaf sheaths
  • Together with the characteristic (22) Na(+) allocation in the blade of the developing immature leaf in the mutants, these results suggest a novel function of OsHKT1;5 in mediating Na(+) exclusion in the phloem to prevent Na(+) transfer to young leaf blades
  • Our findings further demonstrate that the function of OsHKT1;5 is crucial over growth stages of rice, including the protection of the next generation seeds as well as of vital leaf blades under salt stress
  • Immuno-staining using an anti-OsHKT1;5 peptide antibody indicated that OsHKT1;5 is localized in cells adjacent to the xylem in roots
  • Furthermore, OsHKT1;5 was indicated to present in the plasma membrane and found to localize also in the phloem of diffuse vascular bundles in basal nodes
  • Salt tolerance QTL analysis of rice has revealed that the SKC1 locus, which is involved in a higher K(+) /Na(+) ratio in shoots, corresponds to the OsHKT1;5 gene encoding a Na(+) -selective transporter
  • However, physiological roles of OsHKT1;5 in rice exposed to salt stress remain elusive and no OsHKT1;5 gene disruption mutants have been characterized to date
  • Salt stress-induced increases in the OsHKT1;5 transcript was observed in roots and basal stems including basal nodes
  • We also report the development and validation of a new Cleavage Amplified Polymorphic Sequence (CAPS) marker (OsHKT1;5V395) that targets a codon in the sodium transporter gene OsHKT1;5 (Saltol/SKC1 locus) that is associated with sodium transport rates in the above rice landraces
  • Analysis of genetic diversity and population structure using SSR markers and validation of a Cleavage Amplified Polymorphic Sequences (CAPS) marker involving the sodium transporter OsHKT1;5 in saline tolerant rice (Oryza sativa L.) landraces.
  • Allelic variation in rice OsHKT1;5 sequence in specific landraces (Nona Bokra OsHKT1;5-NB/Nipponbare OsHKT1;5-Ni) correlates with variation in salt tolerance
  • In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance
  • Changes in Expression Level of OsHKT1;5 Alters Activity of Membrane Transporters Involved in K + and Ca 2+ Acquisition and Homeostasis in Salinized Rice Roots
  • Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • Using a previously generated high-throughput activation tagging-based T-DNA insertion mutant pool, we isolated a mutant exhibiting salt stress-sensitive phenotype, caused by a reduction in OsHKT1;5 transcripts
  • Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation.
  • The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5
• Connection
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHA1~OsSOS1, Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice Oryza sativa L., In addition, lower expression of OsHKT1;5 and OsSOS1 in old leaves may decrease frequency of retrieving Na+ from old leaf cells
  • OsHKT1;1~OsHKT4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress, Here we present a review on vital physiological functions of HKT transporters including AtHKT1;1 and OsHKT1;5 in preventing shoot Na(+) over-accumulation by mediating Na(+) exclusion from xylem vessels in the presence of a large amount of Na(+) thereby protecting leaves from salinity stress
  • OsHKT1;4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing, Amongst these transporters, the cereal HKT1;4 and HKT1;5 are responsible for Na(+) exclusion from photosynthetic tissues, a key mechanism for plant salinity tolerance
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsPEX11, OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa., Furthermore, qPCR data suggested that OsPEX11 acted as a positive regulator of salt tolerance by reinforcing the expression of several well-known rice transporters (OsHKT2;1, OsHKT1;5, OsLti6a, OsLti6b, OsSOS1, OsNHX1, and OsAKT1) involved in Na(+)/K(+) homeostasis in transgenic plants under salinity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsJRL, OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice., Overexpression of OsJRL in rice also enhanced salinity tolerance and increased the expression levels of a number of stress-related genes, including three LEA (late embryogenesis abundant proteins) genes (OsLEA19a, OsLEA23 and OsLEA24), three Na(+) transporter genes (OsHKT1;3, OsHKT1;4 and OsHKT1;5) and two DREB genes (OsDREB1A and OsDREB2B)
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Here, we show evidence that a rice Mg transporter OsMGT1 is required for the salt-tolerance probably by regulating transport activity of OsHKT1;5, a key transporter for the removal of Na+ from the xylem sap at the root mature zone
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Furthermore, knockout of OsHKT1;5 in osmgt1 mutant background did not further increase its salt sensitivity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMGT1, A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice., Taken together, our results suggest that Mg2+ transported by OsMGT1 in the root mature zone is required for enhancing OsHKT1;5 activity, thereby restricting Na accumulation to the shoots
  • OsHAK1, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Variation in the Abundance of OsHAK1 Transcript Underlies the Differential Salinity Tolerance of an indica and a japonica Rice Cultivar., At the transcriptome level, the over-expression of OsHAK1 stimulated a number of stress-responsive genes as well as four genes known to be involved in Na+ homeostasis and the salinity response (OsHKT1;5, OsSOS1, OsLti6a and OsLti6b)
  • OsHKT1;4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Constitutive Contribution by the Rice OsHKT1;4 Na + Transporter to Xylem Sap Desalinization and Low Na + Accumulation in Young Leaves Under Low as High External Na + Conditions, Three rice HKT genes, OsHKT1;1, OsHKT1;4 and OsHKT1;5, are known to contribute to salt tolerance by reducing Na+ accumulation in shoots upon salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSUVH7, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Using comparative interactomics, we isolated two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsMYB106 and OsSUVH7 bound to the MYB binding cis-element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress, Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity
  • OsGrx_C7, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsHKT2;1~OsHKT1, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHA1~OsSOS1, CC-type glutaredoxin, OsGrx_C7 plays a crucial role in enhancing protection against salt stress in rice, Furthermore, expression analysis suggested that OsGrx_C7 acted as positive regulator of salt tolerance by reinforcing the expression of transporters (OsHKT2;1, OsHKT1;5 and OsSOS1) engaged in Na+ homeostasis in overexpressing plants
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSDG721~SDG721, SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake, RNA sequencing (RNA-seq) analysis of wild-type Kitaake and ossdg721 mutants indicated that OsSDG721 positively regulates the expression level of HIGH-AFFINITY POTASSIUM (K+ ) TRANSPORTER1;5 (OsHKT1;5), which encodes a Na+ -selective transporter that maintains K+ /Na+ homeostasis under salt stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsSDG721~SDG721, SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake, Furthermore, we showed that OsSDG721 binds to and deposits the H3K4me3 mark in the promoter and coding region of OsHKT1;5, thereby upregulating OsHKT1;5 expression under saline-alkaline stress
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNAC3, A NAC transcription factor OsNAC3 positively regulates ABA response and salt tolerance in rice., Furthermore, qRT-PCR and transcriptomic analysis were performed to identify the key OsNAC3 regulated genes related to ABA response and salt tolerance, such as OsHKT1;4, OsHKT1;5, OsLEA3-1, OsPM-1, OsPP2C68, and OsRAB-21
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsMYB106, Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • OsBAG4, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Plasma membrane-localized Hsp40/DNAJ chaperone protein facilitates OsSUVH7-OsBAG4-OsMYB106 transcriptional complex formation for OsHKT1;5 activation., Intriguingly, salt stress facilitates the nuclear relocation of OsDNAJ15 so that it can interact with OsBAG4, and OsDNAJ15 and OsBAG4 synergistically facilitate the DNA-binding activity of OsMYB106 to positively regulate the expression of OsHKT1;5
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsWRKY54, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsWRKY54, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., Furthermore, OsWRKY54 was found to regulate OsHKT1;5 expression by directly binding to the W-box motif in its promoter
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHX4, The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice., The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as OsNHX4 and OsHKT1;5
  • OsAKT2, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
  • OsHAK21~qSE3, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
  • OsHCF222, OsHKT1;5~SKC1~OsHKT8~OsHK1;5, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsHKT2;1~OsHKT1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHA1~OsSOS1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed
  • OsHKT1;5~SKC1~OsHKT8~OsHK1;5, OsNHX1, Natural variations of chlorophyll fluorescence and ion transporter genes influenced the differential response of japonica rice germplasm with different salt tolerances., The expression characteristics of chlorophyll fluorescence genes (OsABCI7 and OsHCF222) and ion transporter protein genes (OsHKT1;5, OsHKT2;1, OsHAK21, OsAKT2, OsNHX1, and OsSOS1) were analyzed

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