OsFBK1

Information
- Symbol: OsFBK1
- MSU: LOC_Os01g47050
- RAPdb: Os01g0659900
Publication
Genbank accession number
Key message
- OsFBK1 was also functionally validated in susceptible PB1 rice cultivar and Arabidopsis for providing drought tolerance
- The OsFBK1 E3 ligase subunit affects anther and root secondary cell wall thickenings by mediating turn-over of a cinnamoyl-CoA reductase.
- Rice transgenics of OsFBK1 displayed variations in anther and root secondary cell wall content; it could be corroborated by electron/confocal microscopy and lignification studies, with no apparent changes in auxin content/signalling pathway
- Interestingly, the OsCCR knock-down transgenics also displayed a decrease in root and anther lignin depositions, suggesting that OsFBK1 plays a role in the development of rice anthers and roots by regulating the cellular levels of a key enzyme controlling lignification
- We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin-5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER-LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1)
- The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets.
- Loss, reduction or gain of function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight
Connection
- OsFBK1, Pb1, Analysis of drought-responsive signalling network in two contrasting rice cultivars using transcriptome-based approach., OsFBK1 was also functionally validated in susceptible PB1 rice cultivar and Arabidopsis for providing drought tolerance
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets.
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., ERECTA PANICLE 3 (EP3) and ORYZA SATIVA F-BOX KELCH 1 (OsFBK1) proteins share 57% and 54% sequence identity with the Arabidopsis F-box protein HAWAIIAN SKIRT (HWS)
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Here we demonstrate that OsFBK1 is another functional orthologue of HWS and show the complexity of interaction between EP3 and OsFBK1 genes at different developmental stages of the plant
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., qRT-PCR expression analyses and studies of EP3-GFP and OsFBK1-RFP promoter reporter lines demonstrate that although EP3 and OsFBK1 expression can be detected in the same tissues some cells exclusively express EP3 or OsFBK1 whilst others co-express both genes
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Loss, reduction or gain of function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin-5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER-LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1)
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., OsNAC1 transcripts are modified by OsFBK1, suggesting two independent regulatory pathways, one via EP3 and OsMIR164 and the other via OsFBK1
- EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Our data propose EP3 and OsFBK1 conjointly play similar roles in rice as HWS does in Arabidopsis
- OsATL53, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification.
- OsATL53, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., SCF OsFBK1 mediates turn-over of OsATL53 in cytoplasm and nucleus, while of OsCCR14 in the nucleus as validated by cell-free degradation assays
- OsATL53, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., These data show OsATL53 affects activity of OsCCR14, and their JA induced degradation by SCF OsFBK1 regulates lignification of rice anthers and roots
- OsCCR14, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification.
- OsCCR14, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., We have shown earlier the F-box protein, OsFBK1, mediating turn-over of a cinnamoyl CoA-reductase, OsCCR14, to regulate rice anther and root lignification
- OsCCR14, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., SCF OsFBK1 mediates turn-over of OsATL53 in cytoplasm and nucleus, while of OsCCR14 in the nucleus as validated by cell-free degradation assays
- OsCCR14, OsFBK1, SCF OsFBK1 E3 ligase mediates jasmonic acid induced turn-over of OsATL53 and OsCCR14 to regulate rice anther and root lignification., These data show OsATL53 affects activity of OsCCR14, and their JA induced degradation by SCF OsFBK1 regulates lignification of rice anthers and roots

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