OsMKKK70

| Categories genes  | Tags leaf  grain  grain size  Kinase  protein kinase  erect  kinase  leaf angle  grain shape  pollen  stress  seed  tolerance  cold tolerance  cold stress  cold  ga  ga  GA  Pollen Fertility  seed set 
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  • Key message
    • OsMKKK70 regulates grain size and leaf angle in rice through the OsMKK4-OsMAPK6-OsWRKY53 signaling pathway.
    • Here we demonstrate that the Oryza sativa Mitogen Activated Protein Kinase Kinase Kinase OsMKKK70 regulates grain size and leaf angle in rice
    • The osmkkk62/70 double mutant and the osmkkk55/62/70 triple mutant displayed significantly smaller seeds and a more erect leaf angle compared to the wild type, indicating that OsMKKK70 functions redundantly with its homologs OsMKKK62 and OsMKKK55
    • Taken together, these findings suggest that OsMKKK70 might regulates grain size and leaf angle in rice by activating OsMAPK6 and that OsMKKK70, OsMKK4, OsMAPK6, and OsWRKY53 function in a common signaling pathway that controls grain shape and leaf angle
    • Biochemical analysis demonstrated that OsMKKK70 is an active kinase and that OsMKKK70 interacts with OsMKK4 and promotes OsMAPK6 phosphorylation
    • In this study, a function of OsMKKK70 (Mitogen Activated Protein Kinase Kinase Kinase 70) in response to CTB was characterized
    • OsMKKK70 overexpression (OsMKKK70-OE) plants were more sensitive to cold stress at the booting stage with a lower seed setting and pollen fertility, but there was no significant difference between the osmkkk70 mutant and WT
    • Considering the effect of functional redundancy, we further tested the CTB response of osmkkk62/70 and osmkkk55/62/70, the double and triple mutants of OsMKKK70 with its closest homologs OsMKKK62 and OsMKKK55, and found that osmkkk62/70 and osmkkk55/62/70 displayed significantly increased CTB with a higher seed setting and pollen fertility, indicating that OsMKKK70 negatively regulates rice CTB
    • OsMKKK70 expression was rapidly induced by cold stress at the booting stage
    • OsMKKK70 Negatively Regulates Cold Tolerance at Booting Stage in Rice.
    • Together, these findings suggest that OsMKKK70 negatively regulates rice CTB by fine-tuning GA levels in anthers
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