| Categories genes  | Tags spikelet  fertility  map-based cloning  height 
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  • Key message
    • The MSI1 member OsRBAP1 gene, identified by a modified MutMap method, is required for rice height and spikelet fertility.
    • Altogether, our results suggest that OsRBAP1 plays an important role in the regulation of rice height and spikelet fertility
    • The CRISPR/Cas9 system was used to switch off OsRBAP1 in PTWT to obtain the knockout mutant osrbap1, which exhibited a severe reduction in height and fertility
    • MutMap-based cloning revealed the occurrence of a single amino acid substitution in the LOC_Os01g51300 gene, belonging to the MSI1 (multicopy suppressor of IRA1) member OsRBAP1
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