| Categories genes  | Tags shoot  root  transcription factor  homeostasis  breeding  phosphorus  pi  phosphate 
  • Information
  • Publication
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  • Key message
    • In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation
    • Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts
    • In vivo root 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root-associated APase
    • Overexpression of OsPAP10a, a root-associated acid phosphatase, increased extracellular organic phosphorus utilization in rice
    • The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis
    • These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use
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