OsAUX1

• Symbol: OsAUX1
• MSU: LOC_Os01g63770
• RAPdb: Os01g0856500

• OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, 2012, PLoS One.
• Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, 2011, J Integr Plant Biol.
• OsAUX1 controls lateral root initiation in rice Oryza sativa L.., 2014, Plant Cell Environ.
• The auxin transporter, OsAUX1, is involved in primary root and root hair elongation and in Cd stress responses in rice Oryza sativa L.., 2015, Plant J.
• Dynamic Regulation of Auxin Response during Rice Development Revealed by Newly Established Hormone Biosensor Markers., 2017, Front Plant Sci.
• Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate., 2018, Nat Commun.
• The WUSCHEL-related homeobox transcription factor OsWOX4 controls the primary root elongation by activating OsAUX1 in rice, 2020, Plant Science.
• MiR529a controls plant height, tiller number, panicle architecture and grain size by regulating SPL target genes in rice Oryza sativa L., 2021, Plant Sci.
• The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes, 2020, Plant Commun.

Genbank accession number

• The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAUX1 controls lateral root initiation in rice (Oryza sativa L.).
• The auxin influx carriers in Arabidopsis have been shown to control lateral root development and gravitropism, but little is known about these proteins in rice
• Three OsAUX1 T-DNA insertion mutants and RNAi knockdown transgenic plants reduced lateral root initiation compared to WT plants
• OsAUX1 overexpression plants exhibited increased lateral root initiation and OsAUX1 was highly expressed in lateral roots and lateral root primordia
• Similarly, the auxin reporter, DR5-GUS, was expressed at lower levels in osaux1 than in the WT plants, which indicated that the auxin levels in the mutant roots had decreased
• Overall, our results indicated that OsAUX1 was involved in polar auxin transport and functioned to control auxin-mediated lateral root initiation in rice
• Polar auxin transport, mediated by influx and efflux transporters, controls many aspects of plant growth and development
• Exogenous NAA treatment rescued the defective phenotype in osaux1-1 plants, whereas IAA and 2,4-D could not, which suggested that OsAUX1 was a putative auxin influx carrier
• The transcript levels of several auxin-signaling genes and cell cycle genes significantly declined in osaux1, hinting that the regulatory role of OsAUX1 may be mediated by auxin-signaling and cell cycle genes
• OsAUX1 expression in root hair cells is different from its paralogous gene, AtAUX1, expressed in non-hair cells
• Taken together, our results indicate that OsAUX1 plays an important role in root development and in responses to Cd stress
• However, OsAUX1 - like AtAUX1 - also localizes at the plasma membrane and seems to function as an auxin tranporter
• Decreased auxin distribution and contents in osaux1 mutant result in reduction of OsCyCB1;1 expression and shortened PR, LR and RH under Cd stress, but can be rescued by the membrane-permeable auxin, 1-naphthaleneacetic acid (NAA)
• Here, we characterize rice osaux1 mutants that have longer PR and shorter RH in hydroponic culture, and that are more sensitive to Cd stress compared to WT/Dongjin (DJ)
• Cd contents in the osaux1 mutant were not altered but reactive oxygen species (ROS)-mediated damage was enhanced, further increasing the osaux1 mutant sensitivity to Cd stress
• Moreover, protein localization of auxin transporters PIN1 homologs and GFP tagged OsAUX1 overlapped with DR5-VENUS during spikelet development, helping validate these auxin response reporters are reliable markers in rice
• Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate.
• Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency
• We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve
• Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation
• We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P
• We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice
• The WUSCHEL-related homeobox transcription factor OsWOX4 controls the primary root elongation by activating OsAUX1 in rice

• OsAUX1, OsTB1~FC1~SCM3~MP3, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAUX1, OsTIR1, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAFB2, OsAUX1, OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice, The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Here, we report that overexpression of OsAGAP, an ARF-GTPase-activating protein in rice, stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells, and was only slightly promoted when the actin filaments were completely disrupted by Lat B
• OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Thus, we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption
• OsAUX1, OsWOX4, The WUSCHEL-related homeobox transcription factor OsWOX4 controls the primary root elongation by activating OsAUX1 in rice, The WUSCHEL-related homeobox transcription factor OsWOX4 controls the primary root elongation by activating OsAUX1 in rice
• OsAUX1, OsWOX4, The WUSCHEL-related homeobox transcription factor OsWOX4 controls the primary root elongation by activating OsAUX1 in rice, Transient transcriptional activation and electrophoretic mobility shift assays showed that OsWOX4 directly regulated the transcription of OsAUX1 through binding to its promoter region
• OsAUX1, OsRLR4, OsRLR4 binds to the OsAUX1 promoter to negatively regulate primary root development in rice., OsRLR4 binds to the OsAUX1 promoter to negatively regulate primary root development in rice.
• OsAUX1, OsRLR4, OsRLR4 binds to the OsAUX1 promoter to negatively regulate primary root development in rice., A series of biochemical and genetic analyses demonstrated that OsRLR4 functions directly upstream of the auxin transporter OsAUX1
• OsAUX1, OsRLR4, OsRLR4 binds to the OsAUX1 promoter to negatively regulate primary root development in rice., Moreover, OsRLR4 interacts with the TRITHORAX-like protein OsTrx1 to promote H3K4me3 deposition at the OsAUX1 promoter, thus altering its transcription level
• OsAUX1, OsUBC11, Ubiquitin-Conjugating Enzyme OsUBC11 Affects the Development of Roots via Auxin Pathway., Expression of the auxin synthesis regulating gene OsYUCCA4/6/7/9, the auxin transport gene OsAUX1, auxin/indole-3-acetic acid (Aux/IAA) family gene OsIAA31, auxin response factor OsARF16 and root regulator key genes, including OsWOX11, OsCRL1, OsCRL5 was significantly down-regulated in OsUBC11 overexpressing plants