OsNAC6,SNAC2

2015-01-20 | Categories genes  | Tags biotic stress  yield  seedling  jasmonic acid  salinity  growth  transcription factor  reproductive  salt  defense response  drought  disease  blast disease  salt tolerance  defense  abiotic stress  jasmonic  root  blast  grain  development  tolerance  grain yield  drought tolerance  stress  drought stress  drought stress 

• Information
  • Symbol: OsNAC6,SNAC2
  • MSU: LOC_Os01g66120
  • RAPdb: Os01g0884300
• Publication
  • The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, 2010, Mol Genet Genomics.
  • OsNAC6, a member of the NAC gene family, is induced by various stresses in rice, 2005, Genes Genet Syst.
  • Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice, 2008, Plant Mol Biol.
  • Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice, 2007, Plant J.
  • The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, 2009, The Plant Journal.
  • The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance., 2016, Plant Biotechnol J.
  • Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., 2023, Genet Mol Biol.
• Genbank accession number
  • AK068392
• Key message
  • Our results imply that OsNAC6, besides having a role in plant adaptation to abiotic stresses, also integrates signals derived from both abiotic and biotic stresses
  • Transgenic rice plants over-expressing OsNAC6 constitutively exhibited growth retardation and low reproductive yields
  • The root phenotype of OsNAC6 knock-out seedlings was observed to be similar to that of the OsHDAC1(OE) seedlings
  • Conversely, the root phenotype of the OsNAC6 overexpressors was similar to that of the OsHDAC1 knock-out seedlings
  • These observations indicate that OsHDAC1 negatively regulates the OsNAC6 gene that primarily mediates the alteration in the root growth of the OsHDAC1(OE) seedlings
  • The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice
  • OsNAC6 was also induced by jasmonic acid (JA), a plant hormone that activates defense responses against herbivores and pathogens
  • Expression of OsNAC6 is induced by abiotic stresses, including cold, drought and high salinity
  • The growth of these plants was similar to that of control plants, whereas the growth of OsNAC6-overexpressing transgenic plants was retarded
  • Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
  • These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice
  • Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice
  • Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment
  • Here we show that OsNAC6 is induced by cold, salt, drought and abscisic acid (ABA)
  • By utilizing stress-inducible promoters, such as the OsNAC6 promoter, it is hoped that stress-inducible over-expression of OsNAC6 in rice can improve stress tolerance by suppressing the negative effects of OsNAC6 on growth under normal growth conditions
  • OsNAC6 gene expression is also induced by wounding and blast disease
  • The OsNAC6 gene is a member of the NAC transcription factor gene family in rice
  • Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice
  • The results of microarray analysis revealed that many genes that are inducible by abiotic and biotic stresses were upregulated in rice plants over-expressing OsNAC6
  • Collectively, these results indicate that OsNAC6 functions as a transcriptional activator in response to abiotic and biotic stresses in plants
  • The rice OsNAC6 transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance.
  • Multiyear drought field tests demonstrated that the grain yield of OsNAC6 root-specific overexpressing transgenic rice lines was less affected by drought stress than were non-transgenic controls
  • Collectively, OsNAC6 orchestrates novel molecular drought tolerance mechanisms and has potential for the biotechnological development of high-yielding crops under water limiting conditions
  • Genome-wide analyses of loss- and gain-of-function mutants revealed that OsNAC6 up-regulates the expression of direct target genes involved in membrane modification, nicotianamine (NA) biosynthesis, glutathione relocation, 3’-phophoadenosine 5’-phosphosulfate accumulation, and glycosylation, which represent multiple drought tolerance pathways
• Connection
  • OsNAC19~SNAC1~OsNAC9, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
  • OsLEA3~OsLEA3-1, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, By gel mobility shift assay, OsNAC5 and OsNAC6 were shown to bind to the OsLEA3 promoter
  • OsLEA3~OsLEA3-1, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
  • OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Pull-down assays revealed that OsNAC5 interacts with OsNAC5, OsNAC6 and SNAC1
  • OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, By gel mobility shift assay, OsNAC5 and OsNAC6 were shown to bind to the OsLEA3 promoter
  • OsNAC5, OsNAC6~SNAC2, The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice, Collectively, our results indicate that the stress-responsive proteins OsNAC5 and OsNAC6 are transcriptional activators that enhance stress tolerance by upregulating the expression of stress-inducible rice genes such as OsLEA3, although the effects of these proteins on growth are different
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, Interestingly, OsNAC6, a member of the NAM-ATAF-CUC (NAC) family, was identified as a key component of the OsHDAC1 regulon, and was found to be epigenetically repressed by OsHDAC1 overexpression
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, The root phenotype of OsNAC6 knock-out seedlings was observed to be similar to that of the OsHDAC1(OE) seedlings
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, Conversely, the root phenotype of the OsNAC6 overexpressors was similar to that of the OsHDAC1 knock-out seedlings
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, These observations indicate that OsHDAC1 negatively regulates the OsNAC6 gene that primarily mediates the alteration in the root growth of the OsHDAC1(OE) seedlings
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, Chromatin immunoprecipitation assays of the OsNAC6 promoter region using antibodies specific to acetylated histones H3 and H4 revealed that OsHDAC1 epigenetically represses the expression of OsNAC6 by deacetylating K9, K14 and K18 on H3 and K5, K12 and K16 on H4
  • OsHDAC1, OsNAC6~SNAC2, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice, The histone deacetylase OsHDAC1 epigenetically regulates theOsNAC6gene that controls seedling root growth in rice
  • OsNAC6~SNAC2, OsTZF5, Expression of the CCCH-tandem zinc finger protein gene OsTZF5 under a stress-inducible promoter mitigates the effect of drought stress on rice grain yield under field conditions., By introducing OsTZF5 behind the stress-responsive OsNAC6 promoter in two commercial upland cultivars, Curinga and NERICA4, we obtained transgenic plants that showed no growth retardation
  • OsFLP, OsNAC6~SNAC2, A Rice R2R3-Type MYB Transcription Factor OsFLP Positively Regulates Drought Stress Response via OsNAC., The yeast-one-hybrid assay indicated that OsFLP can specifically bind and positively regulate OsNAC1 and OsNAC6
  • OsFLP, OsNAC6~SNAC2, A Rice R2R3-Type MYB Transcription Factor OsFLP Positively Regulates Drought Stress Response via OsNAC., These findings suggested that OsFLP positively participates in drought stress, mainly through regulating regulators' transcripts of OsNAC1 and OsNAC6
  • OsNAC5, OsNAC6~SNAC2, Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice (Oryza sativa L.) ionome.
  • OsNAC5, OsNAC6~SNAC2, Enhanced expression of OsNAC5 leads to up-regulation of OsNAC6 and changes rice Oryza sativa L. ionome., In addition, we evaluated the expression level of OsNAC6, which is co-expressed with OsNAC5, and found that enhanced expression of OsNAC5 leads to increased expression of OsNAC6, suggesting that OsNAC5 might regulate OsNAC6 expression

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