OsHAK5

2015-01-20 | Categories genes  | Tags potassium  salt tolerance  transporter  salt  xylem  root  tolerance  stress  epidermis  auxin  tiller  yield  architecture  auxin transport  lateral root  tiller number  root hair  resistance  reactive oxygen species  development  grain  fertility  pollen  seed  grain yield  plasma membrane  pollen development  pollen wall  growth  potassium uptake 

• Information
  • Symbol: OsHAK5
  • MSU: LOC_Os01g70490
  • RAPdb: Os01g0930400
• Publication
  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells, 2011, J Biosci Bioeng.
  • Inventory and functional characterization of the HAK potassium transporters of rice, 2002, Plant Physiol.
  • The role of a potassium transporter OsHAK5 in potassium acquisition and transport from roots to shoots in rice at low potassium supply levels., 2014, Plant Physiol.
  • The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes, 2020, Plant Commun.
  • Calcium lignosulfonate improves proliferation of recalcitrant indica rice callus via modulation of auxin biosynthesis and enhancement of nutrient absorption, 2021, Plant Physiol Biochem.
  • Overexpression of OsHAK5 potassium transporter enhances virus resistance in rice Oryza sativa., 2022, Mol Plant Pathol.
  • Function of Rice HAK Transporters in Pollen Development and Fertility., 2022, Plant Cell Physiol.
  • Rice OsHAK5 is a major potassium transporter that functions in potassium uptake with high specificity but contributes less to cesium uptake., 2022, Biosci Biotechnol Biochem.
• Genbank accession number
  • AP004330
• Key message
  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells
  • These results suggest that the plasma-membrane localized Na(+) insensitive K(+) transporters, similar to OsHAK5 identified here, could be used as a tool to enhance salt tolerance in plant cells
  • Here, in search for Na(+)-insensitive K(+) transporter for this purpose, bacterial expression system was used to characterize two K(+) transporters, OsHAK2 and OsHAK5, isolated from rice (Oryza sativa cv
  • Under the condition with a large amount of extracellular Na(+), we found that OsHAK5 functions as a Na(+)-insensitive K(+) transporter, while OsHAK2 is sensitive to extracellular Na(+) and exhibits higher Na(+) over K(+) transport activities
  • The contribution of OsHAK5 to K mobilization within the rice plant was confirmed further by the change of K concentration in the xylem sap and K distribution in the transgenic lines when K was removed completely from the external solution
  • A β-glucuronidase reporter driven by the OsHAK5 native promoter indicated OsHAK5 expression in various tissue organs from root to seed, abundantly in root epidermis and stele, the vascular tissues, and mesophyll cells
  • Overexpression of OsHAK5 increased the K-sodium concentration ratio in the shoots and salt stress tolerance (shoot growth), while knockout of OsHAK5 decreased the K-sodium concentration ratio in the shoots, resulting in sensitivity to salt stress
  • The role of a potassium transporter OsHAK5 in potassium acquisition and transport from roots to shoots in rice at low potassium supply levels.
  • Here, we show that inactivation of OsHAK5, a rice K+ transporter gene, decreased rootward and shootward PAT, tiller number, and the length of both lateral roots and root hairs, while OsHAK5 overexpression increased PAT, tiller number, and root hair length, irrespective of the K+ supply
  • The Potassium Transporter OsHAK5 Alters Rice Architecture via ATP-Dependent Transmembrane Auxin Fluxes
  • These findings highlight the dual roles of OsHAK5 in altering cellular chemiosmotic gradients (generated continuously by PM H+-ATPase) and regulating ATP-dependent auxin transport
  • Both functions may underlie the prominent effect of OsHAK5 on rice architecture, which may be exploited in the future to increase crop yield via genetic manipulations
  • Overexpression of OsHAK5 potassium transporter enhances virus resistance in rice (Oryza sativa).
  • In contrast, overexpression of OsHAK5 enhanced resistance to RGSV infection
  • The accumulation of ROS is perhaps involved in the induction of host resistance against RGSV infection in OsHAK5 transgenic overexpression rice plants
  • These findings indicate the crucial role of OsHAK5 in host resistance to virus infection
  • Our analysis of reactive oxygen species (ROS) including H(2) O(2) and O(2-) , by DAB and NBT staining, respectively, indicated that RGSV infection as well as OsHAK5 overexpression increased ROS accumulation in rice leaves
  • The results suggest that the role of OsHAK26 in maintaining pollen development and fertility may relate to its proper cargo sorting for construction of pollen walls, while the role of OsHAK1 and OsHAK5 in maintaining seed production likely relates to their transcellular K+ transport activity
  • Seed-setting rate assay by reciprocal cross-pollination between the mutants of oshak26, oshak1, oshak5 and their wild types confirmed that each HAK transporter is foremost for pollen viability, seed-setting and grain yield
  • Knockout of either OsHAK26 or plasma membrane located H+-K+ symporter gene OsHAK1 or OsHAK5 in both Nipponbare and Dongjin cultivars caused distorted anthers, reduced number and germination rate of pollen grains
  • In vitro assay revealed that the pollen germination rate of oshak5 was dramatically affected by external K+ concentration
  • 0 m m and less K, the growth of KD was significantly suppressed, suggesting that OsHAK5 greatly contributed to K absorption under limited K conditions
  • Rice OsHAK5 is a major potassium transporter that functions in potassium uptake with high specificity but contributes less to cesium uptake.
• Connection
  • OsHAK2, OsHAK5, Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells, Here, in search for Na(+)-insensitive K(+) transporter for this purpose, bacterial expression system was used to characterize two K(+) transporters, OsHAK2 and OsHAK5, isolated from rice (Oryza sativa cv
  • OsHAK2, OsHAK5, Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells, Under the condition with a large amount of extracellular Na(+), we found that OsHAK5 functions as a Na(+)-insensitive K(+) transporter, while OsHAK2 is sensitive to extracellular Na(+) and exhibits higher Na(+) over K(+) transport activities
  • OsHAK5, OsSIDP366, OsSIDP366, a DUF1644 gene, positively regulates responses to drought and salt stresses in rice, Digital gene expression (DGE) profile analysis indicated that stress related genes such as SNAC1, OsHAK5 and PRs were up-regulated in OsSIDP366-overexpressing plants.
  • OsAKT1, OsHAK5, Calcium lignosulfonate improves proliferation of recalcitrant indica rice callus via modulation of auxin biosynthesis and enhancement of nutrient absorption, Consistently, increased of potassium protein kinases genes (OsAKT1, OsHAK5, OsCBL, OsCIPK23 and OsCamk1) were also recorded
  • OsCIPK23, OsHAK5, Calcium lignosulfonate improves proliferation of recalcitrant indica rice callus via modulation of auxin biosynthesis and enhancement of nutrient absorption, Consistently, increased of potassium protein kinases genes (OsAKT1, OsHAK5, OsCBL, OsCIPK23 and OsCamk1) were also recorded
  • OsHAK26, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Knockout of either OsHAK26 or plasma membrane located H+-K+ symporter gene OsHAK1 or OsHAK5 in both Nipponbare and Dongjin cultivars caused distorted anthers, reduced number and germination rate of pollen grains
  • OsHAK26, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Seed-setting rate assay by reciprocal cross-pollination between the mutants of oshak26, oshak1, oshak5 and their wild types confirmed that each HAK transporter is foremost for pollen viability, seed-setting and grain yield
  • OsHAK26, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Intriguingly, the pollens of oshak26 showed much thinner wall and were more vulnerable to desiccation than those of oshak1 or oshak5
  • OsHAK26, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., The results suggest that the role of OsHAK26 in maintaining pollen development and fertility may relate to its proper cargo sorting for construction of pollen walls, while the role of OsHAK1 and OsHAK5 in maintaining seed production likely relates to their transcellular K+ transport activity
  • OsHAK1, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Knockout of either OsHAK26 or plasma membrane located H+-K+ symporter gene OsHAK1 or OsHAK5 in both Nipponbare and Dongjin cultivars caused distorted anthers, reduced number and germination rate of pollen grains
  • OsHAK1, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Seed-setting rate assay by reciprocal cross-pollination between the mutants of oshak26, oshak1, oshak5 and their wild types confirmed that each HAK transporter is foremost for pollen viability, seed-setting and grain yield
  • OsHAK1, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., Intriguingly, the pollens of oshak26 showed much thinner wall and were more vulnerable to desiccation than those of oshak1 or oshak5
  • OsHAK1, OsHAK5, Function of Rice HAK Transporters in Pollen Development and Fertility., The results suggest that the role of OsHAK26 in maintaining pollen development and fertility may relate to its proper cargo sorting for construction of pollen walls, while the role of OsHAK1 and OsHAK5 in maintaining seed production likely relates to their transcellular K+ transport activity
  • GORI~OsABT, OsHAK5, OsABT Is Involved in Abscisic Acid Signaling Pathway and Salt Tolerance of Roots at the Rice Seedling Stage., Root ion concentrations indicated that OsABT overexpression lines could maintain lower Na(+) and higher K(+)/Na(+) ratios and upregulated expression of salt-related genes OsSOS1 and OsHAK5 compared with the wild-type (WT) Nipponbare plants
  • OsHAK5, OsIAA25, The suppressed expression of a stress responsive gene 'OsDSR2' enhances rice tolerance in drought and salt stress., , OsHAK5, OsIAA25) and the down-regulation DEGs (e

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