OsCLP

2015-01-20 | Categories genes  | Tags defense  cell wall  growth  root  shoot  seed  starch  yield  stress  lateral root  calcium 

• Information
  • Symbol: OsCLP
  • MSU: LOC_Os01g71080
  • RAPdb: Os01g0937050
• Publication
  • Characterization of a newly identified rice chitinase-like protein OsCLP homologous to xylanase inhibitor, 2013, BMC Biotechnol.
  • A secreted chitinase-like protein OsCLP supports root growth through calcium signaling in Oryza sativa., 2017, Physiol Plant.
• Genbank accession number
  • BAB89707
• Key message
  • These data suggest that OsCLP, which has chitinase activity, may play an important role in plant defenses against pathogens
  • In addition, exogenous treatment with OsCLP affected the growth of the basidiomycete fungus Rhizoctonia solani through degradation of the hyphal cell wall
  • A T-DNA insertion mutant of OsCLP (osclp) showed significant retardation of root and shoot growth
  • A comparative proteomic analysis was carried out using root tissue of wild-type and the osclp mutant to understand the OsCLP-mediated rice growth retardation
  • Exogenous application of Ca(2+) resulted in successful recovery of both primary and lateral root growth in osclp
  • Moreover, overexpression of OsCLP resulted in improved growth with modified seed shape and starch structure; however, the overall yield remained unaffected
  • Taken together, our results highlight the involvement of OsCLP in rice growth by regulating the intracellular calcium concentrations
  • Results obtained revealed that proteins related to glycolysis (phosphoglycerate kinase), stress adaption (chaperonin) and calcium signaling (calreticulin and CDPK1) were differentially regulated in osclp roots
  • Fura-2 molecular probe staining, which is an intracellular calcium indicator, and ICP-MS analysis suggested that the intracellular calcium content was significantly lower in roots of osclp as compared to the wild-type
• Connection

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