Osg1

• Symbol: Osg1
• MSU: LOC_Os01g71930
• RAPdb: Os01g0947700

• A rice beta-1,3-glucanase gene Osg1 is required for callose degradation in pollen development, 2011, Planta.
• Molecular Cloning and Characterization of a Novel β-1,3-Glucanase Gene from Rice, 2014, Bioscience, Biotechnology and Biochemistry.
• Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq, 2020, R Soc Open Sci.

Genbank accession number

• We isolated a cDNA for β-1,3-glucanase from rice anther and named it Oryza sativa glucanase 1 (Osg1)
• RT-PCR analysis revealed that Osg1 transcripts were present in leaves, roots, and anthers
• Osg1 was found to be expressed throughout the plant and highly expressed in florets, leaf sheaths, and leaf blades
• The pollen mother cells appeared to be normal in Osg1-RI plants, but callose degradation was disrupted around the microspores in the anther locules of the Osg1-RI plants at the early microspore stage
• Investigations using real-time PCR, immunocytochemical analysis, and a GUS-reporter gene driven by the Osg1 promoter indicated that Osg1 was mainly expressed at the late meiosis, early microspore, and middle microspore stages in the florets
• The silencing of Osg1 resulted in male sterility
• A rice beta-1,3-glucanase gene Osg1 is required for callose degradation in pollen development

• EG1~GY1, Osg1, A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice Oryza sativa L.., Furthermore, we found that numerous environmentally responsive genes including many floral identity genes are transcriptionally repressed in eg1 mutants and OsMADS1, OsMADS6 and OsG1 genetically act downstream of EG1 to maintain floral robustness