- Information
- Symbol: OsIRO2,OsbHLH056
- MSU: LOC_Os01g72370
- RAPdb: Os01g0952800
- Publication
- Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, 2011, J Exp Bot.
- Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, 2006, J Exp Bot.
- A receptor-like protein RMC is involved in regulation of iron acquisition in rice, 2013, J Exp Bot.
- The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions, 2007, Plant J.
- The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, 2009, Plant J.
- OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil, 2011, Plant Mol Biol.
- A transcription factor OsbHLH156 regulates Strategy II iron acquisition through localizing IRO2 to the nucleus in rice., 2019, New Phytol.
- Enhancement of Iron Acquisition in Rice by the Mugineic Acid Synthase Gene With Ferric Iron Reductase Gene and OsIRO2 Confers Tolerance in Submerged and Nonsubmerged Calcareous Soils., 2019, Front Plant Sci.
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., 2022, Plant J.
- Genbank accession number
- Key message
- Of these genes, a putative basic helix-loop-helix (bHLH) transcription factor gene, named OsIRO2, was strongly expressed in both roots and shoots during Fe deficiency stress
- OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots and leaves, and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in response to Fe deficiency
- Microarray analysis demonstrated that OsIRO2 regulates 59 Fe-deficiency-induced genes in roots
- Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
- RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
- To elucidate the regulation mechanisms of the genes related to Fe acquisition in graminaceous plants, we characterized the Fe-deficiency-inducible basic helix-loop-helix transcription factor OsIRO2 in rice
- Some of these genes, including two transcription factors upregulated by Fe deficiency, possessed the OsIRO2 binding sequence in their upstream regions
- We propose a novel gene regulation network for Fe-deficiency responses, including OsIRO2, IDEs and the two transcription factors
- Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcription factor, OsIRO2, is responsible for regulation of the genes involved in Fe homeostasis in rice
- Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination
- OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil
- These results suggest that OsIRO2 is synchronously expressed with genes involved in Fe homeostasis, and performs a crucial function in regulation not only of Fe uptake from soil but also Fe transport during germination and Fe translocation to grain during seed maturation
- OsIRO2 expression was also detected in flowers and developing seeds
- In this study, we produced a new Fe deficiency-tolerant rice by the additional introduction of a barley IDS3 genome fragment with refre1/372 and OsIRO2 (named as IRI lines) for further enhancement in Strategy II phytosiderophore productivity and better growth performance in various environments
- Our results show that an enhanced tolerance was observed in OsIRO2 introduced line at the early growth stage, refre1/372 introduced line in the late stage, and RI line in all stages among five types of cultivation method
- Enhancement of Iron Acquisition in Rice by the Mugineic Acid Synthase Gene With Ferric Iron Reductase Gene and OsIRO2 Confers Tolerance in Submerged and Nonsubmerged Calcareous Soils.
- Moreover, we demonstrated that new IRI rice lines exhibited enhanced tolerance to Fe deficiency compared to nontransgenic (NT) rice and rice lines harboring the overexpressing OsIRO2 or the IDS3 fragment under submerged calcareous soil
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2
- Connection
- OsIRO2~OsbHLH056, OsNAS2, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
- OsIRO2~OsbHLH056, OsNAS2, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
- OsIRO2~OsbHLH056, OsIRT1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
- OsIRO2~OsbHLH056, OsIRT1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
- OsIRO2~OsbHLH056, OsYSL15, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
- OsIRO2~OsbHLH056, OsYSL15, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
- OsIRO2~OsbHLH056, OsNAS1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2, nicotianamine synthases 1 and 2 (NAS1 and NAS2), yellow-stripe like transporter 15 (YSL15) and iron-regulated transporter (IRT1) indicated that ethylene caused an increase in transcript abundance of both Fe (II) and Fe (III)-phytosiderophore uptake systems
- OsIRO2~OsbHLH056, OsNAS1, Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa, RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1, OsNAS2, OsYSL15, and OsIRT1
- OsIRO2~OsbHLH056, OsNAS1, OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil, Using promoter-GUS transformants, we showed that OsIRO2 is expressed throughout a plant’s lifetime in a spatially and temporally similar manner to the genes OsNAS1, OsNAS2 and TOM1, which is involved in Fe absorption and translocation
- OsIRO2~OsbHLH056, OsNAS2, OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil, Using promoter-GUS transformants, we showed that OsIRO2 is expressed throughout a plant’s lifetime in a spatially and temporally similar manner to the genes OsNAS1, OsNAS2 and TOM1, which is involved in Fe absorption and translocation
- OsIRO2~OsbHLH056, OsNAS1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
- OsIRO2~OsbHLH056, OsNAS3, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
- OsIRO2~OsbHLH056, OsIRT1, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
- OsAPT1~APRT, OsIRO2~OsbHLH056, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
- FDH, OsIRO2~OsbHLH056, Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants, Sequences similar to the OsIRO2-binding sequence were found upstream of several genes that are involved in Fe acquisition, such as OsNAS1, OsNAS3, OsIRT1, OsFDH, OsAPT1, and IDS3
- OsIRO2~OsbHLH056, OsNAAT1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- OsIRO2~OsbHLH056, OsNAS1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- OsIRO2~OsbHLH056, OsNAS2, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- OsIRO2~OsbHLH056, OsIRT1, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- OsIRO2~OsbHLH056, OsRMC~OsRLK, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- OsIRO2~OsbHLH056, OsYSL15, A receptor-like protein RMC is involved in regulation of iron acquisition in rice, Several Fe deficiency-responsive genes including OsDMAS1, OsNAS1, OsNAS2, OsNAAT1, OsIRT1, OsYSL15, and OsIRO2 were up- and downregulated in OsRMC-overexpressing and RNAi plants compared with WT rice plants
- IDEF1, OsIRO2~OsbHLH056, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsIRT1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsNAS2, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsYSL15, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsNAS1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsNAS3, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsYSL2~CF1, The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes, Time-course expression analyses using quantitative reverse-transcriptase PCR revealed that the IDEF1 expression level was positively correlated with the level of induction of the Fe utilization-related genes OsIRO2, OsYSL15, OsIRT1, OsYSL2, OsNAS1, OsNAS2, OsNAS3 and OsDMAS1, just after the onset of Fe starvation
- OsIRO2~OsbHLH056, OsRab6a, A Small GTPase, OsRab6a, Is Involved in the Regulation of Iron Homeostasis in Rice, Exposure to Fe-deficient medium led to up-regulation of OsIRO2, OsIRT1, OsNAS1 and OsNAS2 in both wild-type and the transgenic rice plants, with the magnitude of up-regulation positively correlated with the expression levels of OsRab6a
- OsHRZ2, OsIRO2~OsbHLH056, Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice Oryza sativa L.., C1 (OE) rice plants modify the expression of the putative iron-sensing factors OsHRZ1 and OsHRZ2 and enhance the expression of OsIRO2 under copper excess, which suggests a role of copper transport in iron signaling
- OsHRZ1, OsIRO2~OsbHLH056, Copper and ectopic expression of the Arabidopsis transport protein COPT1 alter iron homeostasis in rice Oryza sativa L.., C1 (OE) rice plants modify the expression of the putative iron-sensing factors OsHRZ1 and OsHRZ2 and enhance the expression of OsIRO2 under copper excess, which suggests a role of copper transport in iron signaling
- OsIRO2~OsbHLH056, OsPRI1~OsbHLH115, POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 OsPRI1 positively regulates iron homeostasis in rice., Yeast one-hybrid and electrophoretic mobility shift assay results suggested that OsPRI1 binds to the OsIRO2 and OsIRO3 promoters
- OsIRO2~OsbHLH056, OsPRI1~OsbHLH115, POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 OsPRI1 positively regulates iron homeostasis in rice., Collectively, these results imply that OsPRI1, which is ubiquitinated by OsHRZ1, mediates rice responses to Fe-deficiency by positively regulating OsIRO2 and OsIRO3 expression as part of the OsHRZ1-OsPRI1-OsIRO2/3 signal transduction cascade
- OsbHLH59~OsbHLH059~OsPRI3, OsIRO2~OsbHLH056, Oryza sativa POSITIVE REGULATOR OF IRON DEFICIENCY RESPONSE 2 OsPRI2 and OsPRI3 are involved in the maintenance of Fe homeostasis., We also provide evidence that OsPRI2 and OsPRI3 bind to the promoters of OsIRO2 and OsIRO3, two key regulators of Fe homeostasis
- OsIRO2~OsbHLH056, OsIRT1, Enhancement of Iron Acquisition in Rice by the Mugineic Acid Synthase Gene With Ferric Iron Reductase Gene and OsIRO2 Confers Tolerance in Submerged and Nonsubmerged Calcareous Soils., Furthermore, under Fe-limited nonsubmerged calcareous soil condition (a new cultivation condition), IRI lines also conferred enhanced tolerance than NT, lines introducing only the OsIRT1 promoter-refre1/372 or overexpressing OsIRO2, and lines harboring both
- OsIRO2~OsbHLH056, SH5~RI, Enhancement of Iron Acquisition in Rice by the Mugineic Acid Synthase Gene With Ferric Iron Reductase Gene and OsIRO2 Confers Tolerance in Submerged and Nonsubmerged Calcareous Soils., Our results show that an enhanced tolerance was observed in OsIRO2 introduced line at the early growth stage, refre1/372 introduced line in the late stage, and RI line in all stages among five types of cultivation method
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., Further analysis revealed that OsIRO3 directly regulated the expression of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2), which encodes a positive regulator of the Fe uptake system
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., OsIRO3 repressed their transcription activation towards OsIRO2
- OsbHLH061~OsbHLHq11, OsIRO2~OsbHLH056, OsbHLH061 links TOPLESS/TOPLESS-RELATED repressor proteins with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 to maintain iron homeostasis in rice., Mechanistically, OsbHLH061 expression is upregulated by high Fe, and physically interacts with OsPRI1, the OsbHLH061-OsPRI1 complex recruits TOPLESS/TOPLESS-RELATED (OsTPL/TPR) co-repressors to repress OsIRO2 and OsIRO3 expression
Prev Next