OsSGL

2015-01-20 | Categories genes  | Tags growth  grain  development  grain number  grain length  yield  cell division  cytokinin  grain filling  grain weight  root  drought  tolerance  drought tolerance  stress  nucleus  stress tolerance  transcription factor  starch  quality  homeostasis  endosperm  sucrose  grain quality  starch biosynthesis  amylose content  sucrose synthase 

• Information
  • Symbol: OsSGL
  • MSU: LOC_Os02g04130
  • RAPdb: Os02g0134200
• Publication
  • OsSGL, a novel pleiotropic stress-related gene enhances grain length and yield in rice., 2016, Sci Rep.
  • OsSGL, a Novel DUF1645 Domain-Containing Protein, Confers Enhanced Drought Tolerance in Transgenic Rice and Arabidopsis., 2016, Front Plant Sci.
  • Transcription factor OsSGL is a regulator of starch synthesis and grain quality in rice., 2022, J Exp Bot.
• Genbank accession number
  • AK108331
• Key message
  • Together, our results suggest that OsSGL may regulate stress-tolerance and cell growth by acting via a cytokinin signaling pathway
  • Overexpression of OsSGL significantly altered certain development processes greatly and positively affecting an array of traits in transgenic rice plants, including increased grain length, grain weight and grain number per panicle, resulting in a significant increase in yield
  • Microscopical analysis showed that the enhanced OsSGL expression promoted cell division and grain filling
  • These results suggest that the mechanism by which OsSGL confers enhanced drought tolerance is due to the modulated expression of stress-responsive genes, higher accumulations of osmolytes, and enlarged root systems
  • We investigated OsSGL (Oryza sativa Stress tolerance and Grain Length), a novel DUF1645 domain-containing protein from rice
  • Transgenic plants over-expressing or hetero-expressing OsSGL conferred significantly improved drought tolerance in transgenic rice and Arabidopsis thaliana, respectively
  • OsSGL was up-regulated by multiple stresses and localized to the nucleus
  • Transcription factor OsSGL is a regulator of starch synthesis and grain quality in rice.
  • Therefore, our findings demonstrate that accurate control of OsSGL homeostasis is essential for starch synthesis and grain quality
  • In addition, we revealed the molecular mechanism of OsSGL in regulating starch biosynthesis-related genes, which are required for grain quality
  • Overexpression of OsSGL reduced total starch and amylose content in the endosperm compared with the wild type
  • Chromatin immunoprecipitation sequencing and RNA-seq analyses indicated that OsSGL targets the transcriptional activity of several starch and sucrose metabolism genes
  • Unexpectedly, our results also show that knock down and mutation of OsSGL disrupts the starch biosynthetic pathway, causing lower starch and amylose content
  • In addition, ChIP-qPCR, yeast one-hybrid, EMSA and dual-luciferase assays demonstrated that OsSGL directly inhibits the expression of SUCROSE SYNTHASE 1 (OsSUS1) in the endosperm
• Connection
  • OsSGL, OsSOG1, SUPPRESSOR OF GAMMA RESPONSE 1 plays rice-specific roles in DNA damage response and repair., Rice SOG1 (OsSOG1) and SOG1-like (OsSGL) were identified as putative AtSOG1 orthologs with complete or partial conservation of the serine-glutamine (SQ) motifs involved in activation via phosphorylation
  • OsSGL, OsSOG1, SUPPRESSOR OF GAMMA RESPONSE 1 plays rice-specific roles in DNA damage response and repair., In addition to OsSOG1 or OsSGL knockout mutants, OsSOG1 non-phosphorylatable mutants (OsSOG1-7A) were generated by homologous recombination-mediated gene targeting (GT)
  • OsSGL, OsSOG1, SUPPRESSOR OF GAMMA RESPONSE 1 plays rice-specific roles in DNA damage response and repair., Based on the analysis of DNA damage susceptibility and the effect on the expression of DNA repair-related genes using these mutants, we have demonstrated that OsSOG1 plays a more important role than OsSGL in controlling the DDR and DNA repair

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