OsAGAP

2015-01-20 | Categories genes  | Tags primary root  root  seedling  root development  growth  auxin  lateral root  iaa  adventitious root  grain  grain yield  yield  architecture  plant architecture 

• Information
  • Symbol: OsAGAP
  • MSU: LOC_Os02g10480
  • RAPdb: Os02g0198300
• Publication
  • Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, 2011, J Integr Plant Biol.
  • OsAGAP, an ARF-GAP from rice, regulates root development mediated by auxin in Arabidopsis, 2005, Plant Cell Environ.
  • Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development, 2006, Plant J.
  • OsPDCD5 negatively regulates plant architecture and grain yield in rice, 2021, Proc Natl Acad Sci U S A.
• Genbank accession number
  • AK060556
• Key message
  • Transgenic Arabidopsis with OsAGAP constitutively expression showed reduced apical dominance, shorter primary roots, increasing number of longer adventitious roots
  • Here, we show that over-expression of OsAGAP, an ARF-GTPase-activating protein (ARF-GAP) in rice, impaired PAT and interfered with both primary and lateral root development
  • Together, our data suggest that OsAGAP has a specific role in regulating vesicle trafficking pathways such as the auxin influx pathway, which in turn controls auxin-dependent root growth in plants
  • Over-expression of OsAGAP, an ARF-GAP, interferes with auxin influx, vesicle trafficking and root development
  • Determination of whole-plant IAA level showed that there is a sharp increase of free IAA in OsAGAP transgenic Arabidopsis seedlings
  • These results suggest OsAGAP, an ARF-GAP of rice, maybe involved in the mediation of plant root development by regulating auxin level
  • OsAGAP, an ARF-GAP from rice, regulates root development mediated by auxin in Arabidopsis
  • Thus, we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption
  • OsPDCD5 interacted directly with OsAGAP, and OsAGAP positively regulated plant architecture and grain yield in rice
• Connection
  • OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Here, we report that overexpression of OsAGAP, an ARF-GTPase-activating protein in rice, stimulates vesicle transport from the plasma membrane to the Golgi apparatus in protoplasts and transgenic plants and induces the accumulation of early endosomes and AUX1
  • OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Fluorescence recovery after photobleaching analysis revealed exocytosis of the AUX1 recycling endosome was not affected in the OsAGAP overexpression cells, and was only slightly promoted when the actin filaments were completely disrupted by Lat B
  • OsAGAP, OsAUX1, Adenosine diphosphate ribosylation factor-GTPase-activating protein stimulates the transport of AUX1 endosome, which relies on actin cytoskeletal organization in rice root development, Thus, we propose that AUX1 accumulation in the OsAGAP overexpression and actin disrupted cells may be due to the fact that endocytosis of the auxin influx carrier AUX1 early endosome was greatly promoted by actin cytoskeleton disruption
  • OsAGAP, OsPDCD5, OsPDCD5 negatively regulates plant architecture and grain yield in rice, OsPDCD5 interacted directly with OsAGAP, and OsAGAP positively regulated plant architecture and grain yield in rice

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