- Information
- Symbol: GluB-1
- MSU: LOC_Os02g15169
- RAPdb: Os02g0249900
- Publication
- Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene GluB-1, 1999, Plant Mol Biol.
- The 3’-untranslated region of rice glutelin GluB-1 affects accumulation of heterologous protein in transgenic rice, 2009, Biotechnol Lett.
- Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, 2014, Plant Mol Biol.
- Genbank accession number
- Key message
- Accumulation of mDer f 2 in transgenic seed and leaf using the GluB-1 terminator was greater than when using the Nos terminator construct
- The mDer f 2 mRNA containing the GluB-1 3’UTR was processed and polyadenylated at the same sites as the native GluB-1 mRNA in the seeds but diverged in leaves of the transgenic plants
- In this study, we describe a zinc finger gene of the Cys3His1 (CCCH or C3H) class, named OsGZF1, which was identified in a yeast one-hybrid screening using the core promoter region of GluB-1 as bait and cDNA expression libraries prepared from developing rice panicles and grains as prey
- The promoter region of GluB-1, one of the glutelin genes in rice, has been intensively used as a model to understand regulation of seed-storage protein accumulation
- Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice
- Therefore, we conclude that at least three cis-regulatory elements, the AACA motif, GCN4 motif and ACGT motif, are necessary to mediate endosperm expression of the GluB-1 glutelin gene
- Identification of cis-regulatory elements required for endosperm expression of the rice storage protein glutelin gene GluB-1
- We propose that OsGZF1 has a function in regulating the GluB-1 promoter and controls accumulation of glutelins during grain development
- Connection
- GluB-1, OsGZF1, Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, In this study, we describe a zinc finger gene of the Cys3His1 (CCCH or C3H) class, named OsGZF1, which was identified in a yeast one-hybrid screening using the core promoter region of GluB-1 as bait and cDNA expression libraries prepared from developing rice panicles and grains as prey
- GluB-1, OsGZF1, Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, Transient expression experiments demonstrated that OsGZF1 can down-regulate a GluB-1-GUS (beta-glucuronidase) reporter and OsGZF1 was also able to significantly reduce activation conferred by RISBZ1 which is a known strong GluB-1 activator
- GluB-1, OsGZF1, Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, We propose that OsGZF1 has a function in regulating the GluB-1 promoter and controls accumulation of glutelins during grain development
- GluB-1, OsGZF1, Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice, Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice
- GluB-1, OsPht1;8~OsPT8, Phosphate transporter OsPht1;8 in rice plays an important role in phosphorus redistribution from source to sink organs and allocation between embryo and endosperm of seeds., In this study, we knocked down expression of a phosphate transporter gene OsPht1;8 (OsPT8) selectively in shoot and/or in seed endosperm by RNA-interference using RISBZ1 and GluB-1 promoter (designate these transgenic lines as SSRi and EnSRi), respectively, to characterize the role of OsPT8 in P redistribution of rice
- GluB-1, OsGLK1, Rice carotenoid biofortification and yield improvement conferred by endosperm-specific overexpression of OsGLK1., Transgenic overexpression of all four genes (OsGLK1, tHMG1, ZmPSY1, and PaCrtI) driven by rice endosperm-specific promoter GluB-1 established a mini carotenoid biosynthetic pathway in the endosperm and exerted a roughly multiplicative effect on the carotenoid accumulation as compared with the overexpression of only three genes (tHMG1, ZmPSY1, and PaCrtI)
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