- Information
- Symbol: RPBF,OsDof3
- MSU: LOC_Os02g15350
- RAPdb: Os02g0252400
- Publication
- Identification of Dof proteins with implication in the gibberellin-regulated expression of a peptidase gene following the germination of rice grains, 2001, Biochim Biophys Acta.
- Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone, 2003, Plant Physiol.
- Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, 2010, Plant Cell Physiol.
- Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, 2009, Plant J.
- SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, 2014, Mol Plant.
- Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, 2006, Plant Physiol.
- Genbank accession number
- Key message
- These phenotypes suggest that combinatorial interactions between RISBZ1 and RPBF play an essential role during grain filling
- Compensation and interaction between RISBZ1 and RPBF during grain filling in rice
- The Dof (DNA binding with one finger) transcriptional activator rice (Oryza sativa) prolamin box binding factor (RPBF), which is involved in gene regulation of rice seed storage proteins, has been isolated from rice cDNA expressed sequence tag clones containing the conserved Dof
- We demonstrated by transient expression in rice callus protoplasts that the isolated RPBF trans-activated several storage protein genes via an AAAG target sequence located within their promoters, and with methylation interference experiments the additional AAAG-like sequences in promoters of genes expressed in maturing seeds were recognized by the RPBF protein
- The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1
- Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes
- Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone
- The results showed the accessory function of OsDOF3 responsible for a dosage-dependent mediation of GA signaling that leads to high-level expression of physiological target genes
- ) basic leucine Zipper factor RISBZ1 and rice prolamin box binding factor (RPBF) are transcriptional activators of rice seed storage protein (SSP) genes in vivo
- To ascertain the functions of these trans-activators in seed development, knock-down (KD) transgenic rice plants were generated in which the accumulation of RISBZ1 and RPBF was reduced in an endosperm-specific manner by co-suppression (KD-RISBZ1 and KD-RPBF)
- Storage lipids were accumulated at reduced levels in KD-RISBZ1 and KD-RISBZ1/KD-RPBF seeds
- KD-RPBF and KD-RISBZ1/KD-RPBF seeds exhibited multi-layered aleurone cells
- The functional redundancy and compensation between RISBZ1 and RPBF possibly account for weak effects on the SSP levels in single KD mutants, and help maintain various processes during seed development in rice
- Through genome-wide expression profiling and chromatin immunoprecipitation, we found that SERF1 directly regulates RICE PROLAMIN-BOX BINDING FACTOR (RPBF), a TF that functions as a positive regulator of grain filling
- Our study reveals that SERF1 represents a negative regulator of grain filling and seedling establishment by timing the expression of RPBF
- Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- Loss of SERF1 enhances RPBF expression resulting in larger grains with increased starch content, while SERF1 overexpression represses RPBF resulting in smaller grains
- This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- Connection
- OsMYBGA~OsGAMYB, RPBF~OsDof3, Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone, In this study, I have evaluated cooperative function of GAMYB and a pyrimidine box-binding protein OsDOF3 that influenced the promoter activity of the most predominant GA-responsive gene (RAmy1A) of rice (Oryza sativa)
- OsMYBGA~OsGAMYB, RPBF~OsDof3, Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone, Transient expression of OsDOF3 in the germinated aleurone prolonged GAMYB function on the reporter expression in the absence of GA
- OsMYBGA~OsGAMYB, RPBF~OsDof3, Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone, The yeast two-hybrid assay showed the physical interaction of GAMYB and OsDOF3 in yeast cells, indicating that the association of GAMYB and OsDOF3 may be a functional unit in transcription regulation
- AmyI-1~RAmy1A, RPBF~OsDof3, Functional dissections between GAMYB and Dof transcription factors suggest a role for protein-protein associations in the gibberellin-mediated expression of the RAmy1A gene in the rice aleurone, In this study, I have evaluated cooperative function of GAMYB and a pyrimidine box-binding protein OsDOF3 that influenced the promoter activity of the most predominant GA-responsive gene (RAmy1A) of rice (Oryza sativa)
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- OsSAL1~RHL, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, ) basic leucine Zipper factor RISBZ1 and rice prolamin box binding factor (RPBF) are transcriptional activators of rice seed storage protein (SSP) genes in vivo
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, To ascertain the functions of these trans-activators in seed development, knock-down (KD) transgenic rice plants were generated in which the accumulation of RISBZ1 and RPBF was reduced in an endosperm-specific manner by co-suppression (KD-RISBZ1 and KD-RPBF)
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, The accumulation of most SSPs changed little between individual KD mutants and wild-type plants, whereas a double KD mutant (KD-RISBZ1/KD-RPBF) resulted in a significant reduction of most SSP gene expression and accumulation
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Storage lipids were accumulated at reduced levels in KD-RISBZ1 and KD-RISBZ1/KD-RPBF seeds
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, KD-RPBF and KD-RISBZ1/KD-RPBF seeds exhibited multi-layered aleurone cells
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, These phenotypes suggest that combinatorial interactions between RISBZ1 and RPBF play an essential role during grain filling
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, The functional redundancy and compensation between RISBZ1 and RPBF possibly account for weak effects on the SSP levels in single KD mutants, and help maintain various processes during seed development in rice
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Physical interaction between RISBZ1 and RPBF may ensure that these processes are carried out properly
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice
- OsCR4~MIS2, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- ADL1~OsDEK1, RPBF~OsDof3, Compensation and interaction between RISBZ1 and RPBF during grain filling in rice, Gene expression of DEFECTIVE KERNEL1 (OsDEK1), CRINKLY4 (OsCR4) and SUPERNUMERARY ALEURONE LAYER 1 (OsSAL1) rice homologues was decreased in the KD mutants, suggesting that these genes are regulated by RISBZ1 and RPBF
- AmyI-1~RAmy1A, RPBF~OsDof3, SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, In addition, SERF1 negatively regulates germination by controlling RPBF expression, which mediates the gibberellic acid (GA)-induced expression of RICE AMYLASE1A (RAmy1A)
- RPBF~OsDof3, SERF1, SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, Through genome-wide expression profiling and chromatin immunoprecipitation, we found that SERF1 directly regulates RICE PROLAMIN-BOX BINDING FACTOR (RPBF), a TF that functions as a positive regulator of grain filling
- RPBF~OsDof3, SERF1, SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, Loss of SERF1 enhances RPBF expression resulting in larger grains with increased starch content, while SERF1 overexpression represses RPBF resulting in smaller grains
- RPBF~OsDof3, SERF1, SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, In addition, SERF1 negatively regulates germination by controlling RPBF expression, which mediates the gibberellic acid (GA)-induced expression of RICE AMYLASE1A (RAmy1A)
- RPBF~OsDof3, SERF1, SALT-RESPONSIVE ERF1 Is a Negative Regulator of Grain Filling and Gibberellin-Mediated Seedling Establishment in Rice, Our study reveals that SERF1 represents a negative regulator of grain filling and seedling establishment by timing the expression of RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Synergism between RPBF and RISBZ1 recognizing the GCN4 motif [TGA(G/C)TCA] was observed in the expression of many storage protein genes
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Overexpression of both transcription factors gave rise to much higher levels of expression than the sum of individual activities elicited by either RPBF or RISBZ1 alone
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Furthermore, mutation of recognition sites suppressed reciprocal trans-activation ability, indicating that there are mutual interactions between RISBZ1 and RPBF
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, The RPBF gene is predominantly expressed in maturing endosperm and coordinately expressed with seed storage protein genes, and is involved in the quantitative regulation of genes expressed in the endosperm in cooperation with RISBZ1
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes, Synergism between RPBF Dof and RISBZ1 bZIP activators in the regulation of rice seed expression genes
- RISBZ1~OsbZIP58~OsSMF1, RPBF~OsDof3, Genome-wide identification of grain filling genes regulated by the OsSMF1 transcription factor in rice., OsSMF1 was shown to bind directly to the promoters of Os03g0168500 (GCN4 motif), patatin-like gene (GCN4 motif), α-globulin (ACGT motif), rice prolamin box-binding factor (RPBF) (ATGA motif), and ONAC024 (GCN4 and ACGT motifs) and to regulate their expression
- OsDML4, RPBF~OsDof3, A new demethylase gene, OsDML4, is involved in high temperature-increased grain chalkiness in rice., The expression of two transcription factor genes RISBZ1 and RPBF was significantly decreased in the osdml4 mutants, which caused adverse effects on the formation of protein bodies (PBs) with greatly decreased PB-II number, and incomplete and abnormally shaped PB-IIs
Prev Next