EP3,LP

2015-01-20 | Categories genes  | Tags erect  vascular bundle  architecture  panicle size  panicle architecture  cytokinin  panicle  grain  leaf  flower  photosynthesis  stomatal  development  root  pollen  grain size  floral  floral organ  flower development  crown  crown root  plant architecture  organ size  floral organ number 

• Information
  • Symbol: EP3,LP
  • MSU: LOC_Os02g15950
  • RAPdb: Os02g0260200
• Publication
  • Map-based cloning of the ERECT PANICLE 3 gene in rice, 2009, Theor Appl Genet.
  • Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice, 2011, Plant Biotechnol J.
  • Decreased photosynthesis in the erect panicle 3 ep3 mutant of rice is associated with reduced stomatal conductance and attenuated guard cell development., 2015, J Exp Bot.
  • The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., 2021, Plant Biotechnol J.
• Genbank accession number
  • AK064350
• Key message
  • Genetic analysis revealed that the erect panicle phenotype was controlled by a single recessive mutation designated erect panicle 3 (ep3)
  • A line carrying a T-DNA insertion in LOC_ Os02g15950 was obtained and shown to have the same phenotype as the ep3 mutant, thus confirming the identification of LOC_Os02g15950 as the ERECT PANICLE 3 (EP3) gene
  • The ep3 mutation causes a significant increase in the number of small vascular bundles as well as the thickness of parenchyma in the peduncle, which results in the erect panicle phenotype
  • We report herein an in-depth characterization of two allelic larger panicle (lp) mutants that show significantly increased panicle size as well as improved plant architecture
  • These results suggest that LP plays an important role in regulating plant architecture, particularly in regulating panicle architecture, thereby representing promising targets for genetic improvement of grain production plants
  • Quantitative real-time PCR results show that OsCKX2, which encodes cytokinin oxidase/dehydrogenase, is down-regulated evidently in mutants, implying that LP might be involved in modulating cytokinin level in plant tissues
  • Map-based cloning reveals that LARGER PANICLE (LP) encodes a Kelch repeat-containing F-box protein
  • This anatomical characteristic may account for the observed decrease in leaf photosynthesis and provides evidence that EP3 plays a role in regulating stomatal guard cell development
  • Ectopic expression of the Os02g15950 coding sequence, driven by the HWS (At3g61950) promoter, rescued the hws-1 flower phenotype in Arabidopsis confirming that EP3 is a functional orthologue of HWS
  • However, both ep3 mutant plants and transgenic lines that have a T-DNA insertion within the Os02g15950 (EP3) gene exhibit smaller stomatal guard cells compared with their wild-type controls
  • We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin-5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER-LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1)
  • The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets.
  • Loss, reduction or gain of function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight
• Connection
  • EP3~LP, Gn1a~OsCKX2, Mutations in the F-box gene LARGER PANICLE improve the panicle architecture and enhance the grain yield in rice, Quantitative real-time PCR results show that OsCKX2, which encodes cytokinin oxidase/dehydrogenase, is down-regulated evidently in mutants, implying that LP might be involved in modulating cytokinin level in plant tissues
  • EP3~LP, OsPIN2~LRA1, Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice., In this study, phenotypic and genetic analyses were undertaken to explore the role of OsPIN2, an auxin efflux transporter, in regulating the growth and development of rice roots under normal nutrition condition (control) and low-phosphate condition (LP)
  • EP3~LP, OsPIN2~LRA1, Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice., Higher expression of OsPIN2 was observed in rice plants under LP compared to the control
  • EP3~LP, OsPIN2~LRA1, Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice., Compared to WT plants, overexpression of OsPIN2 had a shorter root length through decreased root cell elongation under control and LP
  • EP3~LP, OsPIN2~LRA1, Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice., Surprisingly, overexpression of OsPIN2 might increase auxin distribution in epidermis of root, resulting in greater RH formation but less LR development in OE plants than in WT plants in the control condition but levels similar of these under LP
  • EP3~LP, OsPIN2~LRA1, Overexpression of OsPIN2 Regulates Root Growth and Formation in Response to Phosphate Deficiency in Rice., These results suggest that higher OsPIN2 expression regulates rice root growth and development maybe by changing auxin distribution in roots under LP condition
  • EP3~LP, GF14b~OsGF14b, Rice G protein γ subunit qPE9-1 modulates root elongation for phosphorus uptake by involving 14-3-3 protein OsGF14b and plasma membrane H + -ATPase, Moreover, the overexpression of OsGF14b in WYJ8 (carrying the qpe9-1 allele) partially increased primary root length under LP conditions
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets.
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., ERECTA PANICLE 3 (EP3) and ORYZA SATIVA F-BOX KELCH 1 (OsFBK1) proteins share 57% and 54% sequence identity with the Arabidopsis F-box protein HAWAIIAN SKIRT (HWS)
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Here we demonstrate that OsFBK1 is another functional orthologue of HWS and show the complexity of interaction between EP3 and OsFBK1 genes at different developmental stages of the plant
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., qRT-PCR expression analyses and studies of EP3-GFP and OsFBK1-RFP promoter reporter lines demonstrate that although EP3 and OsFBK1 expression can be detected in the same tissues some cells exclusively express EP3 or OsFBK1 whilst others co-express both genes
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Loss, reduction or gain of function lines for EP3 and OsFBK1, show that EP3 and OsFBK1 affect plant architecture, organ size, floral organ number and size, floral morphology, pollen viability, grain size and weight
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., We have identified the putative orthologue genes of the rice microRNA pathway for ORYZA SATIVA DAWDLE (OsDDL) and ORYZA SATIVA SERRATE (OsSE), and demonstrated that EP3 and OsFBK1 affect their transcript levels as well as those of CROWN ROOT DEFECT 1/ORYZA SATIVA Exportin-5 HASTY (CRD1/OsHST), ORYZA SATIVA DICER-LIKE 1 (OsDCL) and ORYZA SATIVA WEAVY LEAF1 (OsWAF1)
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., OsNAC1 transcripts are modified by OsFBK1, suggesting two independent regulatory pathways, one via EP3 and OsMIR164 and the other via OsFBK1
  • EP3~LP, OsFBK1, The Rice EP3 and OsFBK1 E3 ligases alter plant architecture and flower development, and affect transcript accumulation of microRNA pathway genes and their targets., Our data propose EP3 and OsFBK1 conjointly play similar roles in rice as HWS does in Arabidopsis

Prev     Next