OsIPK2,IPK2,IP6K,ES2

2015-01-20 | Categories genes  | Tags mitochondria  anther  root  auxin  lateral root  auxin response  leaf  leaf senescence  early leaf senescence  senescence  tillering  growth  seedling  photosynthesis  nucleus  plant growth  Kinase  plasma membrane  chlorophyll content  reactive oxygen species  kinase 

• Information
  • Symbol: OsIPK2,IPK2,IP6K,ES2
  • MSU: LOC_Os02g32370
  • RAPdb: Os02g0523800
• Publication
  • IP6K gene identification in plant genomes by tag searching, 2011, BMC Proc.
  • Expression pattern of inositol phosphate-related enzymes in rice Oryza sativa L.: Implications for the phytic acid biosynthetic pathway, 2007, Gene.
  • Rice Inositol Polyphosphate Kinase OsIPK2 Directly Interacts with OsIAA11 to Regulate Lateral Root Formation., 2017, Plant Cell Physiol.
  • Rice EARLY SENESCENCE 2, encoding an inositol polyphosphate kinase, is involved in leaf senescence, 2020, BMC Plant Biol.
• Genbank accession number
  • AK072296
• Key message
  • CONCLUSIONS: The analysis we conducted in plant mitochondria provided the negative, though we argue relevant, result that IP6K does not actually occur in vegetable mtDNA
  • On the other hand, the up-regulation of a MIPS, an IMP, an IPK2, and an ITP5/6K in anthers suggested that a PLC-mediated pathway was active in addition to a lipid-independent pathway in the anthers
  • Furthermore, expressing additional OsIPK2 or its N-terminal amino acid sequence enhanced the accumulation of OsIAA11 protein in transgenic plants, which in turn caused defects in lateral root formation and auxin response
  • Conclusions: The ES2 gene, encoding an inositol polyphosphate kinase localized in the nucleus and plasma membrane of cells, is essential for leaf senescence in rice
  • Further study of ES2 will facilitate the dissection of the genetic mechanisms underlying early leaf senescence and plant growth
  • Leaves of es2 showed early senescence at the seedling stage and became severe at the tillering stage
  • Moreover, genes which related to senescence, ROS and chlorophyll degradation were up-regulated, while those associated with photosynthesis and chlorophyll synthesis were down-regulated in es2 mutant compared to WYG7
  • DNA sequencing of ES2 in the mutant revealed a missense mutation, ES2 was localized to nucleus and plasma membrane of cells, and expressed in various tissues of rice
  • The ES2 gene, which encodes an inositol polyphosphate kinase (OsIPK2), was fine mapped to a 116
  • The contents of reactive oxygen species (ROS) significantly increased, while chlorophyll content, photosynthetic rate, catalase (CAT) activity significantly decreased in the es2 mutant
  • Complementation test and overexpression experiment confirmed that ES2 completely restored the normal phenotype, with chlorophyll contents and photosynthetic rate increased comparable with the wild type
• Connection
  • OsIAA11, OsIPK2~IPK2~IP6K~ES2, Rice Inositol Polyphosphate Kinase OsIPK2 Directly Interacts with OsIAA11 to Regulate Lateral Root Formation., Rice Inositol Polyphosphate Kinase (OsIPK2) Directly Interacts with OsIAA11 to Regulate Lateral Root Formation.
  • OsIAA11, OsIPK2~IPK2~IP6K~ES2, Rice Inositol Polyphosphate Kinase OsIPK2 Directly Interacts with OsIAA11 to Regulate Lateral Root Formation., Here, we show that OsIPK2, a rice (Oryza sativa) inositol polyphosphate kinase, directly interacts with an Aux/IAA protein OsIAA11 to repress its degradation
  • OsIAA11, OsIPK2~IPK2~IP6K~ES2, Rice Inositol Polyphosphate Kinase OsIPK2 Directly Interacts with OsIAA11 to Regulate Lateral Root Formation., Furthermore, expressing additional OsIPK2 or its N-terminal amino acid sequence enhanced the accumulation of OsIAA11 protein in transgenic plants, which in turn caused defects in lateral root formation and auxin response

Prev     Next