- Information
- Symbol: OsCPS2,OsCyc2
- MSU: LOC_Os02g36210
- RAPdb: Os02g0571100,Os02g0570900
- Publication
- Comparison of the Enzymatic Properties ofent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice, 2014, Bioscience, Biotechnology and Biochemistry.
- Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, 2014, Bioscience, Biotechnology and Biochemistry.
- gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, 2006, Plant, Cell and Environment.
- Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, 2004, Plant J.
- Transcripts of two ent-copalyl diphosphate synthase genes differentially localize in rice plants according to their distinct biological roles, 2014, J Exp Bot.
- Inferring Roles in Defense from Metabolic Allocation of Rice Diterpenoids., 2018, Plant Cell.
- A conditional role for labdane-related diterpenoid natural products in rice stomatal closure, 2021, New Phytol.
- Genbank accession number
- Key message
- Several enzymatic properties of OsCPS2/OsCyc2, including the optimal pH, optimal temperature, divalent cation requirement, and kinetic values for the geranylgeranyl diphosphate (GGDP) substrate, were almost the same as those of OsCPS1
- Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
- The rice genome contains two ent-copalyl diphosphate synthase genes: OsCPS1 acts in gibberellin (phytohormone) biosynthesis, and OsCPS2/OsCyc2 acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins)
- Furthermore, the OsCPS1 activity exhibited approximately 70% inhibition by 100 muM Amo-1618 (a gibberellin biosynthetic inhibitor), whereas the OsCPS2/OsCyc2 activity exhibited approximately 10% inhibition
- The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
- Rice (Oryza sativa L.) has two ent-CDP synthase genes, OsCPS1 and OsCPS2.
- The results indicate that transcripts of the two ent-CDP synthase genes differentially localize in rice plants according to their distinct biological roles, OsCPS1 for growth and OsCPS2 for defence.
- It has been thought that OsCPS1 participates in GA biosynthesis, while OsCPS2 participates in the biosynthesis of phytoalexins, phytocassanes A-E, and oryzalexins A-F.
- Connection
- OsCPS~OsCPS1, OsCPS2~OsCyc2, Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
- OsCPS~OsCPS1, OsCPS2~OsCyc2, Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, In vitro assays using recombinant enzymes enabled three cDNAs encoding ent-copalyl diphosphate synthases to be identified in wheat (Triticum aestivum): TaCPS1, TaCPS2, and TaCPS3
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, Cloning and Characterization of cDNAs Encodingent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes, The phylogenetic tree and expression analyses suggest that TaCPS3 is responsible for gibberellin biosynthesis, while TaCPS1 and TaCPS2 are possible functional homologs of diterpene cyclase genes OsCPS2 and OsCPS4 involved in phytoalexin biosynthesis in rice
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
- GID1~OsGID1, OsCPS2~OsCyc2, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
- OsCPS~OsCPS1, OsCPS2~OsCyc2, gid1, a gibberellin-insensitive dwarf mutant, shows altered regulation of probenazole-inducible protein PBZ1 in response to cold stress and pathogen attack, Specifically, OsCPS1, which encodes an enzyme in the GA biosynthesis pathway, was down-regulated and OsCPS2 and OsCPS4, which encode enzymes in phytoalexin biosynthesis, were up-regulated in gid1
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, To clarify the biological functions of the ent- and syn-CDP synthases involved in the biosynthesis of phytoalexins and/or GAs, we isolated two cDNAs, OsCyc1 and OsCyc2, encoding putative diterpene cyclases from ultraviolet (UV)-irradiated rice leaves (cv
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, Using a bacterial expression system, we demonstrated that OsCyc1 encodes syn-CDP synthase and that OsCyc2 and OsCPS1 encode ent-CDP synthase
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment, whereas expression of the OsCPS1 transcript was not induced by UV light
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, These results suggest that OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs, respectively
- OsCPS~OsCPS1, OsCPS2~OsCyc2, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, Using a bacterial expression system, we demonstrated that OsCyc1 encodes syn-CDP synthase and that OsCyc2 and OsCPS1 encode ent-CDP synthase
- OsCPS~OsCPS1, OsCPS2~OsCyc2, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, The level of expression of the OsCyc1 and OsCyc2 transcripts in rice leaves increased drastically in response to UV treatment, whereas expression of the OsCPS1 transcript was not induced by UV light
- OsCPS~OsCPS1, OsCPS2~OsCyc2, Biological functions of ent- and syn-copalyl diphosphate synthases in rice: key enzymes for the branch point of gibberellin and phytoalexin biosynthesis, These results suggest that OsCyc1, OsCyc2 and OsCPS1 are responsible for the biosynthesis of momilactones A and B and oryzalexin S, oryzalexins A-F and phytocassanes A-E, and GAs, respectively
- DPF, OsCPS2~OsCyc2, Diterpenoid Phytoalexin Factor, a bHLH Transcription Factor, Plays a Central Role in the Biosynthesis of Diterpenoid Phytoalexins in Rice, Furthermore, DPF activated the promoters of COPALYL DIPHOSPHATE SYNTHASE2 (CPS2) and CYTOCHROME P450 MONOOXIGENASE (CYP) 99A2, which are implicated in the biosynthesis of PCs and MLs, respectively.
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, A conditional role for labdane-related diterpenoid natural products in rice stomatal closure, Rice also produces LRD phytoalexins, utilizing OsCPS2 and OsCPS4 to initiate a complex metabolic network
- OsCPS2~OsCyc2, OsCPS4~OsCyc1, A conditional role for labdane-related diterpenoid natural products in rice stomatal closure, For genetic studies of rice LRD biosynthesis the fast-growing Kitaake cultivar was selected for targeted mutagenesis via CRISPR/Cas9, with an initial focus on OsCPS2 and OsCPS4
- D3, OsCPS2~OsCyc2, The miR528-D3 Module Regulates Plant Height in Rice by Modulating the Gibberellin and Abscisic Acid Metabolisms., Moreover, the expression level of GA biosynthesis-related key genes, including OsCPS1, OsCPS2, OsKO2 and OsKAO was remarkably higher in OE-D3 plants, while the NECD2 expression, a key gene involved in ABA biosynthesis, was significantly higher in d3 mutants than in WT and OE-D3 plants
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