OsCER1,OsGL1-4,LTR1

• Symbol: OsCER1,OsGL1-4,LTR1
• MSU: LOC_Os02g40784
• RAPdb: Os02g0621300

• Tapetum degeneration retardation is critical for aliphatic metabolism and gene regulation during rice pollen development, 2008, Mol Plant.
• OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice Oryza sativa L.., 2018, Front Plant Sci.
• Deficiency of very-long-chain alkanes biosynthesis causes humidity-sensitive male sterility via affecting pollen adhesion and hydration in rice., 2019, Plant Cell Environ.
• OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain VLC alkane metabolism of tryphine in rice, 2021, Funct Plant Biol.
• LEAF TIP RUMPLED 1 Regulates Leaf Morphology and Salt Tolerance in Rice., 2022, Int J Mol Sci.

Genbank accession number

• OsCER1 was highly expressed in the tapetum (stage 10) and bicellular pollen cells (stage 11)
• Downregulation of OsCER1 in rice led to sterility, and fewer amyloplasts within the mature pollen grains
• Furthermore, significantly altered levels of expression of genes involved in the pollen development were exhibited in the OsCER1 knocked down plants
• These results indicate that OsCER1 is critical for VLC alkanes biosynthesis, plastids differentiation, and pollen development
• OsCER1 Plays a Pivotal Role in Very-Long-Chain Alkane Biosynthesis and Affects Plastid Development and Programmed Cell Death of Tapetum in Rice (Oryza sativa L.).
• In addition, the downregulation of OsCER1 in rice caused delayed tapetal programmed cell death and abnormal development of plastids in the tapetal cells
• OsGL1-4 is preferentially expressed in pollen and tapetal cells, and is required for the synthesis of very-long-chain alkanes
• Gas chromatography-mass spectrometry analysis suggested the humidity-sensitive male sterility of osgl1-4 was probably due to a significant reduction in C25 and C27 alkanes
• OsCER1 regulates humidity-sensitive genic male sterility through very-long-chain (VLC) alkane metabolism of tryphine in rice
• Phenotypic investigation of NPB and ltr1 suggested that ltr1 showed rumpled leaf with uneven distribution of bulliform cells and sclerenchyma cells, and disordered vascular bundles
• A decrease in seed-setting rate in ltr1 led to decreased per-plant grain yield
• Moreover, ltr1 was sensitive to salt stress, and LTR1 was strongly induced by salt stress
• Moreover, overexpression of LTR1 enhanced yield in rice and LTR1 positively regulates salt stress by affecting water and ion homeostasis
• Map-based cloning of LTR1 showed that there was a 2-bp deletion in the eighth exon of LOC_Os02g40784 in ltr1, resulting in a frameshift mutation and early termination of transcription

• OsCER1~OsGL1-4~LTR1, OsMYB60, Mutation of OsMYB60 reduces rice resilience to drought stress by attenuating cuticular wax biosynthesis., Yeast one-hybrid, luciferase transient transcriptional activity, and chromatin immunoprecipitation assays revealed that OsMYB60 directly binds to the promoter of OsCER1 (a key gene involved in very-long-chain alkane biosynthesis) and upregulates its expression