OsMYB30,OsMYB5P

• Symbol: OsMYB30,OsMYB5P
• MSU: LOC_Os02g41510
• RAPdb: Os02g0624300

• The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression., 2017, Plant Physiol.
• The MAMP-responsive MYB transcription factors MYB30, MYB55, and MYB110 activate the HCAA synthesis pathway and enhance immunity in rice., 2018, Plant Cell Physiol.
• Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter., 2018, PLoS One.
• Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves., 2020, New Phytol.
• OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , 2021, Rice (N Y).

Genbank accession number

• These results suggested that OsMYB30 exhibited a regulatory effect on the breakdown of starch through the regulation of the BMY genes
• These results together suggested that OsMYB30 might be a novel regulator of cold tolerance through the negative regulation of the BMY genes by interacting with OsJAZ9 to fine tune the starch breakdown and the content of maltose which might contribute to the cold tolerance as a compatible solute
• The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression.
• In this study, we characterized a cold-responsive R2R3-type MYB gene OsMYB30 for its regulatory function in cold tolerance in rice (Oryza sativa)
• Functional analysis revealed that overexpression of OsMYB30 in rice resulted in increased cold sensitivity, while the osmyb30 knockout mutant showed increased cold tolerance
• These results indicate that OsMYB5P is associated with the regulation of shoot development and root- system architecture
• OsMYB5P protein is localized to the nucleus and functions as a transcription activator in plant development
• Overexpression of OsMYB5P in rice and Arabidopsis (Arabidopsis thaliana Col-0) increases tolerance to phosphate starvation, whereas OsMYB5P knock-out through RNA interference increases sensitivity to Pi depletion in rice
• Together, these results demonstrate that overexpression of OsMYB5P increases tolerance to Pi deficiency in plants by modulating Pi transporters at the transcriptional level in monocots and dicots
• Overexpression of OsMYB5P led to increased Pi accumulation in shoots and roots
• In addition, overexpression of OsMYB5P in Arabidopsis triggered increased expression of AtPht1;3, an Arabidopsis Pi transporter, in shoots and roots under normal and Pi-deficient conditions
• Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter.
• Here, we report the involvement of the OsMYB30 gene in the bsr-d1-mediated blast resistance
• We further find that OsMYB30 binds to and activates the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S
• It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45-1, OsSRFP1, OsCYL4 and OsMYB30
• Under cold stress treatments, the transcription of four cold-related genes OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT)

• OsJAZ9~OsTIFY11a, OsMYB30~OsMYB5P, The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression., Furthermore, we identified an OsMYB30-interacting protein OsJAZ9 that had a significant effect in suppressing the transcriptional activation of OsMYB30 and in the repression of BMY genes mediated by OsMYB30
• OsJAZ9~OsTIFY11a, OsMYB30~OsMYB5P, The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing beta-amylase expression., These results together suggested that OsMYB30 might be a novel regulator of cold tolerance through the negative regulation of the BMY genes by interacting with OsJAZ9 to fine tune the starch breakdown and the content of maltose which might contribute to the cold tolerance as a compatible solute
• OsMYB30~OsMYB5P, OsPht1;5~OsPT5, Rice OsMYB5P improves plant phosphate acquisition by regulation of phosphate transporter., Interestingly, OsMYB5P directly bound to MBS (MYB binding site) motifs on the OsPT5 promoter and induced transcription of OsPT5 in rice
• OsmiR528, OsMYB30~OsMYB5P, OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells., To investigate the potential mechanism of OsmiR528 in increasing cold stress tolerance, we examined expression of stress-associated MYB transcription factors OsGAMYB-like1, OsMYBS3, OsMYB4, OsMYB3R-2, OsMYB5, OsMYB59, OsMYB30, OsMYB1R, and OsMYB20 in rice cells by qRT-PCR
• OsmiR528, OsMYB30~OsMYB5P, OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells., Our experiments demonstrated that OsmiR528 decreases expression of transcription factor OsMYB30 by targeting a F-box domain containing protein gene (Os06g06050), which is a positive regulator of OsMYB30
• OsmiR528, OsMYB30~OsMYB5P, OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells., In OsmiR528 transgenic rice, reduced OsMYB30 expression results in in-creased expression of BMY genes OsBMY2, OsBMY6, and OsBMY10
• OsmiR528, OsMYB30~OsMYB5P, OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells., The transcript levels of the OsBMY2, OsBMY6, and OsBMY10 were elevated by OsMYB30 knockdown, but decreased by Os-MYB30 overexpression in OsmiR528 transgenic cell lines, suggesting that OsmiR528 increases low temperature tolerance by modulating expression of stress response-related transcription factor
• OsMYB30~OsMYB5P, OsPAL8, An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice., Furthermore, we show that expression of OsPAL6 and OsPAL8 in response to BPH attack is directly up-regulated by OsMYB30, an R2R3 MYB transcription factor
• OsMYB30~OsMYB5P, OsPAL6, An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice., Furthermore, we show that expression of OsPAL6 and OsPAL8 in response to BPH attack is directly up-regulated by OsMYB30, an R2R3 MYB transcription factor
• Os4CL3, OsMYB30~OsMYB5P, Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves., We further find that OsMYB30 binds to and activates the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S
• Os4CL5, OsMYB30~OsMYB5P, Sclerenchyma cell thickening through enhanced lignification induced by OsMYB30 prevents fungal penetration of rice leaves., We further find that OsMYB30 binds to and activates the promoters of 4-coumarate:coenzyme A ligase genes (Os4CL3 and Os4CL5) resulting in accumulation of lignin subunits G and S
• OsMYB30~OsMYB5P, OsSRFP1~SDEL2, OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , Under cold stress treatments, the transcription of four cold-related genes OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT)
• OsMYB30~OsMYB5P, OsSRFP1~SDEL2, OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45-1, OsSRFP1, OsCYL4 and OsMYB30
• ART1, OsMYB30~OsMYB5P, ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification., ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification.
• ART1, OsMYB30~OsMYB5P, ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification., Furthermore, transient dual-luciferase assay showed that ART1 directly inhibited the expression of OsMYB30, and in turn repressed Os4CL5-dependent 4-coumaric acid accumulation, hence reducing the Al-binding capacity of cell wall and enhancing Al resistance