OsDOF11

• Symbol: OsDOF11
• MSU: LOC_Os02g47810
• RAPdb: Os02g0707200

• Rice Transcription Factor OsDOF11 Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and SWEET genes., 2018, Mol Plant.
• OsRRM, an RNA-Binding Protein, Modulates Sugar Transport in Rice Oryza sativa L., 2020, Front Plant Sci.
• OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice Oryza sativa L.., 2021, Front Plant Sci.
• The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., 2022, Plant Physiol.
• OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., 2022, Front Biosci (Landmark Ed).

Genbank accession number

• Rice Transcription Factor OsDOF11 Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and SWEET genes.
• Although sucrose enhanced root elongation in young wild-type (WT) seedlings, this did not occur in osdof11 seedlings due to reduced sucrose uptake
• Sugar transport rate analyses revealed that less sugar was transported in osdof11 plants than in the WT
• We also observed that osdof11 mutant plants were less susceptible to infection by Xanthomonas oryzae pathovar oryzae, suggesting that OsDOF11 participates in sugar distribution during pathogenic invasions
• Collectively, these results suggest that OsDOF11 modulates sugar transport by regulating expression of SUT and SWEET genes in rice
• Chromatin immunoprecipitation experiments showed that OsDOF11 directly binds to the promoter regions of SUT1, OsSWEET11, and OsSWEET14, indicating that OsDOF11 coordinately controls transporters that are responsible for sucrose transport via apoplastic loading
• OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice (Oryza sativa L.).
• Here, we note that OsDOF11 promotes nitrogen uptake and then maintains the ratio of fresh weight to dry weight in seedling plants and the effective leaf blade at flowering stages
• Both sugar and organic nitrogen changes caused the ratio of fresh weight to dry weight to decrease in OsDOF11 mutant seedling plants and mature leaves, which might result in vigorous reduced metabolic activity and become less susceptible to stress
• These results demonstrated that OsDOF11 affected nitrogen metabolism by sugar distribution in rice, which provided new insight that OsDOF11 coordinated with C and N balance to maintain plant growth activity
• Mutants of the sucrose transporter gene OsSWEET14 displayed a phenotype similar to that of OsDOF11
• The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development.
• Here, we found that OsDOF11 loss-of-function mutants showed a semi-dwarf phenotype with a smaller cell length due to increased cytokinin content in source tissues
• RNA sequencing and reverse transcription quantitative PCR analyses revealed that genes involved in cytokinin signaling and metabolism were affected in osdof11 mutants
• sativa VIN3-LIKE 2 caused an increase in active cytokinin levels and induced OsDOF11 transcript levels
• We note that the loss function of OsDOF11 displays the reduced crown roots number, low activity of nitrogen assimilation and low content of cytokinin and auxin
• OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa.
• RESULTS: We demonstrate the role of OsDOF11 in root development
• 0% sucrose (MS30) by OsDOF11 in rice root
• CONCLUSIONS: OsDOF11 promotes crown root formation via cytokinin in oryza sativa
• These results provide a physiological basis for further analysis of the OsDOF11 function of in rice root development
• Exogenous application of cytokinin and [13C] sucrose labeled stable isotope uptake experiments help us to explain the relationship between OsDOF11 and cytokinin

• Os11N3~OsSWEET14, OsDOF11, Rice Transcription Factor OsDOF11 Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and SWEET genes., Chromatin immunoprecipitation experiments showed that OsDOF11 directly binds to the promoter regions of SUT1, OsSWEET11, and OsSWEET14, indicating that OsDOF11 coordinately controls transporters that are responsible for sucrose transport via apoplastic loading
• Os8N3~xa13~OsSWEET11, OsDOF11, Rice Transcription Factor OsDOF11 Modulates Sugar Transport by Promoting Expression of Sucrose Transporter and SWEET genes., Chromatin immunoprecipitation experiments showed that OsDOF11 directly binds to the promoter regions of SUT1, OsSWEET11, and OsSWEET14, indicating that OsDOF11 coordinately controls transporters that are responsible for sucrose transport via apoplastic loading
• Os11N3~OsSWEET14, OsDOF11, OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice Oryza sativa L.., Previously, we noted that Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) mediated sucrose transport by binding to the promoter regions of Sucrose Transporter 1 (SUT1), Oryza sativa Sugars Will Eventually be Exported Transporters 11 (OsSWEET11), and OsSWEET14
• Os11N3~OsSWEET14, OsDOF11, OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice Oryza sativa L.., Mutants of the sucrose transporter gene OsSWEET14 displayed a phenotype similar to that of OsDOF11
• Os8N3~xa13~OsSWEET11, OsDOF11, OsDOF11 Affects Nitrogen Metabolism by Sucrose Transport Signaling in Rice Oryza sativa L.., Previously, we noted that Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) mediated sucrose transport by binding to the promoter regions of Sucrose Transporter 1 (SUT1), Oryza sativa Sugars Will Eventually be Exported Transporters 11 (OsSWEET11), and OsSWEET14
• OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Moreover, mutation of osckx4 in the osdof11 osckx4 double mutant rescued the semi-dwarf phenotype of the osdof11 mutant
• OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Interestingly, exogenous application of kinetin promoted OsDOF11 expression earlier than OsCKX4, and overexpression of O
• OsCKX4, OsDOF11, The sucrose transport regulator OsDOF11 mediates cytokinin degradation during rice development., Taken together, our results suggest a model in which both a sucrose transport regulator (OsDOF11) and cytokinin via OsCKX4 establish a feedback loop to maintain dynamic tissue homeostasis
• OsCKX4, OsDOF11, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., Previously, we reported negative feedback regulation loop between Oryza sativa DNA BINDING WITH ONE FINGER 11 (OsDOF11) and cytokinin by Oryza sativa CYTOKININ OXIDASE/DEHYDROGENASE 4 (OsCKX4) in rice development
• OsCKX4, OsDOF11, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)
• OsDOF11, OsRR2, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)
• OsDOF11, OsRR3, OsDOF11 Promotes Crown Root Formation via Cytokinin in Oryza Sativa., The expression level of WUSCHEL-related homeobox (OsWOX11), A-type response regulator 2 (OsRR2), OsRR3, and OsCKX4 were decreased in osdof11-1, as well as in OsDOF11 RNA interference 9 mutants (RNAi-9 lines)