Ghd2,OsK

• Symbol: Ghd2,OsK
• MSU: LOC_Os02g49880
• RAPdb: Os02g0731700

• Ghd2, a CONSTANS-like gene, confers drought sensitivity through regulation of senescence in rice., 2016, J Exp Bot.
• The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice., 2023, J Genet Genomics.

Genbank accession number

• Here, we found that a CO-like gene, Ghd2 (Grain number, plant height, and heading date2), which can increase the yield potential under normal growth condition just like its homologue Ghd7, is involved in the regulation of leaf senescence and drought resistance
• Ghd2 is expressed mainly in the rice (Oryza sativa) leaf with the highest level detected at the grain-filling stage, and it is down-regulated by drought stress conditions
• The earlier senescence symptoms and the transcript up-regulation of many senescence-associated genes (SAGs) in Ghd2-overexpressing transgenic rice plants under drought stress conditions indicate that Ghd2 plays essential roles in accelerating drought-induced leaf senescence in rice
• Moreover, developmental and dark-induced leaf senescence was accelerated in the Ghd2-overexpressing rice and delayed in the ghd2 mutant
• Overexpression of Ghd2 resulted in significantly reduced drought resistance, while its knockout mutant showed the opposite phenotype
• OsARID3 and OsPURα showed expression patterns similar to Ghd2 in rice leaves, with the highest levels at the grain-filling stage, whereas OsARID3 and the 14-3-3 genes responded differently to drought stress conditions
• These results indicate that Ghd2 functions as a regulator by integrating environmental signals with the senescence process into a developmental programme through interaction with different proteins
• The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice.
• However, the target gene of Ghd2 regulating heading date is still unknown
• A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a, and RFT1
• Taken together, these results suggest that Ghd2 directly binds to the downstream gene CO3, and the Ghd2-CO3 module constantly delays heading date via the Ehd1-mediated pathway

• Ghd2~OsK, Ghd7, Ghd2, a CONSTANS-like gene, confers drought sensitivity through regulation of senescence in rice., Here, we found that a CO-like gene, Ghd2 (Grain number, plant height, and heading date2), which can increase the yield potential under normal growth condition just like its homologue Ghd7, is involved in the regulation of leaf senescence and drought resistance
• Ghd2~OsK, OsARID3, Ghd2, a CONSTANS-like gene, confers drought sensitivity through regulation of senescence in rice., Ghd2 interacted with several regulatory proteins, including OsARID3, OsPURα, and three 14-3-3 proteins
• Ghd2~OsK, OsARID3, Ghd2, a CONSTANS-like gene, confers drought sensitivity through regulation of senescence in rice., OsARID3 and OsPURα showed expression patterns similar to Ghd2 in rice leaves, with the highest levels at the grain-filling stage, whereas OsARID3 and the 14-3-3 genes responded differently to drought stress conditions
• Ghd2~OsK, OsDCL3a, Translational repression by a miniature inverted-repeat transposable element in the 3’ untranslated region., Interestingly, the translational repression of Ghd2 by the sMITE mainly relies on Dicer-like 3a (OsDCL3a)
• Ehd1, Ghd2~OsK, The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice., A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a, and RFT1
• Ghd2~OsK, Hd3a, The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice., A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a, and RFT1
• Ghd2~OsK, RFT1, The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice., A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a, and RFT1