OsbZIP23

2015-01-20 | Categories genes  | Tags drought  drought tolerance  transcription factor  ethylene  ABA  salinity stress  salt  abiotic stress  salinity  leaf  tolerance  yield  reproductive  sugar  ABA  resistance  stress  drought resistance  drought stress  drought stress  Kinase  stress response  protein kinase  ABA biosynthesis  ER stress 

• Information
  • Symbol: OsbZIP23
  • MSU: LOC_Os02g52780
  • RAPdb: Os02g0766700
• Publication
  • Genome-wide analysis of basic leucine zipper transcription factor families in Arabidopsis thaliana, Oryza sativa and Populus trichocarpa, 2009, Journal of Shanghai University (English Edition).
  • Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice, 2012, Plant Physiol.
  • Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice, 2008, Plant Physiol.
  • Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice, 2008, Plant Physiol.
  • Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, 2014, Plant Cell Rep.
  • Enhanced Gene Expression Rather than Natural Polymorphism in Coding Sequence of the OsbZIP23 Determines Drought Tolerance and Yield Improvement in Rice Genotypes., 2016, PLoS One.
  • Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., 2016, Plant Physiol.
  • Synergistic regulation of drought-responsive genes by transcription factor OsbZIP23 and histone modification in rice., 2019, J Integr Plant Biol.
  • OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice , 2020, Plant J..
  • The coordination of OsbZIP72 and OsMYBS2 with reverse roles regulates the transcription of OsPsbS1 in rice, 2021, New Phytol.
  • Rice glycosyltransferase gene UGT2 functions in salt stress tolerance under the regulation of bZIP23 transcription factor., 2022, Plant Cell Rep.
• Genbank accession number
  • AK072062
• Key message
  • Expression of OsbZIP23 is strongly induced by a wide spectrum of stresses, including drought, salt, abscisic acid (ABA), and polyethylene glycol treatments, while other stress-responsive genes of this family are slightly induced only by one or two of the stresses
  • Transgenic rice overexpressing OsbZIP23 showed significantly improved tolerance to drought and high-salinity stresses and sensitivity to ABA
  • On the other hand, a null mutant of this gene showed significantly decreased sensitivity to a high concentration of ABA and decreased tolerance to high-salinity and drought stress, and this phenotype can be complemented by transforming the OsbZIP23 back into the mutant
  • We propose that OsbZIP23 is a major player of the bZIP family in rice for conferring ABA-dependent drought and salinity tolerance and has high potential usefulness in genetic improvement of stress tolerance
  • Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice
  • The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance
  • OsbZIP23 is a member of the basic leucine zipper (bZIP) transcription factor family in rice (Oryza sativa)
  • Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively
  • Collectively, these results indicate that OsbZIP23 functions as a transcriptional regulator that can regulate the expression of a wide spectrum of stress-related genes in response to abiotic stresses through an ABA-dependent regulation pathway
  • The single-copy OsbZIP23 gene is expressed at relatively higher level in leaf tissues of drought-tolerant genotypes, and its abundance is more in reproductive stage
  • Enhanced Gene Expression Rather than Natural Polymorphism in Coding Sequence of the OsbZIP23 Determines Drought Tolerance and Yield Improvement in Rice Genotypes.
  • The overexpression of any of the two polymorphic forms (1083 bp and 1068 bp CDS) of OsbZIP23 improved drought tolerance and yield-related traits significantly by retaining higher content of cellular water, soluble sugar and proline; and exhibited decrease in membrane lipid peroxidation in comparison to RNAi lines and non-transgenic plants
  • Taken together, the present study concludes that the enhanced gene expression rather than natural polymorphism in coding sequence of OsbZIP23 is accountable for improved drought tolerance and yield performance in rice genotypes
  • The OE lines showed higher expression of target genes-OsRab16B, OsRab21 and OsLEA3-1 and increased ABA sensitivity; indicating that OsbZIP23 is a positive transcriptional-regulator of the ABA-signaling pathway
  • The OsbZIP23 transcription factor has been characterized for its essential role in drought resistance in rice, but the mechanism is unknown
  • Together, our results suggest that OsbZIP23 acts as a central regulator in ABA signaling and biosynthesis, and drought resistance in rice
  • Next, we performed genome-wide identification of OsbZIP23 targets by immunoprecipitation sequencing (ChIP-seq) and RNA Sequencing (RNA-Seq) analyses in the OsbZIP23-overexpression, osbzip23 mutant, and wild-type rice under normal and drought stress conditions
  • Among these targets, we found that OsbZIP23 could positively regulate OsPP2C49, and overexpression of OsPP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration
  • Moreover, OsNCED4 (9-cis-epoxycarotenoid dioxygenase 4), a key gene in ABA biosynthesis, was also positively regulated by OsbZIP23
  • OsbZIP23 directly regulates a large number of reported genes that function in stress response, hormone signaling, and developmental processes
  • A homolog of SnRK2 protein kinase SAPK2 was found to interact with and phosphorylate OsbZIP23 for its transcriptional activation
  • Synergistic regulation of drought-responsive genes by transcription factor OsbZIP23 and histone modification in rice.
  • Here, we report an interplay between histone H3K4me3 modification and transcription factor OsbZIP23 in the regulation of a dehydrin gene cluster under drought stress conditions in rice
  • Conversely, the H3K4me3 modification and dehydrin gene expression levels were down-regulated in the osbzip23 mutant under drought stress conditions
  • The transcription factor OsbZIP23 was demonstrated to regulate the UGT2 expression under stress conditions
• Connection
  • OsABF1~OsABI5~OREB1~OsbZIP10, OsbZIP23, Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice, It has high sequence similarity to ABA-responsive element binding factor (ABF/AREB) transcription factors ABI5 and OsbZIP23, two transcriptional activators positively regulating stress tolerance in Arabidopsis (Arabidopsis thaliana) and rice, respectively
  • OsAREB8~OsAREB1~OsbZIP46~OsABF2~ABL1, OsbZIP23, Constitutive activation of transcription factor OsbZIP46 improves drought tolerance in rice, The stress-related genes activated by OsbZIP46CA1 are largely different from those activated by the other rice ABF/AREB homologs (such as OsbZIP23), further implying the value of OsbZIP46CA1 in genetic engineering of drought tolerance
  • OsbZIP23, OsP5CS~OsP5CS1, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsNCED4, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsNAC19~SNAC1~OsNAC9, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsLEA3~OsLEA3-1, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsDSR2, Overexpression of a new stress-repressive gene OsDSR2 encoding a protein with a DUF966 domain increases salt and simulated drought stress sensitivities and reduces ABA sensitivity in rice, Overexpression of OsDSR2 could increase salt and simulated drought (polyethyleneglycol)-stress sensitivities in rice by downregulating the expression of ABA- and stress-responsive genes including OsNCED4, SNAC1, OsbZIP23, P5CS, Oslea3 and rab16C
  • OsbZIP23, OsLEA3~OsLEA3-1, Enhanced Gene Expression Rather than Natural Polymorphism in Coding Sequence of the OsbZIP23 Determines Drought Tolerance and Yield Improvement in Rice Genotypes., The OE lines showed higher expression of target genes-OsRab16B, OsRab21 and OsLEA3-1 and increased ABA sensitivity; indicating that OsbZIP23 is a positive transcriptional-regulator of the ABA-signaling pathway
  • OsbZIP23, OsNCED4, Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., Moreover, OsNCED4 (9-cis-epoxycarotenoid dioxygenase 4), a key gene in ABA biosynthesis, was also positively regulated by OsbZIP23
  • OsbZIP23, SAPK2~OsSAPK2, Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., A homolog of SnRK2 protein kinase SAPK2 was found to interact with and phosphorylate OsbZIP23 for its transcriptional activation
  • OsbZIP23, SAPK2~OsSAPK2, Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., SAPK2 also interacted with OsPP2C49, an ABI1 homolog, which deactivated the SAPK2 to inhibit the transcriptional activation activity of OsbZIP23
  • OsbZIP23, OsPP2C49, Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., SAPK2 also interacted with OsPP2C49, an ABI1 homolog, which deactivated the SAPK2 to inhibit the transcriptional activation activity of OsbZIP23
  • OsbZIP23, OsPP2C49, Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought resistance related genes., Among these targets, we found that OsbZIP23 could positively regulate OsPP2C49, and overexpression of OsPP2C49 in rice resulted in significantly decreased sensitivity of the ABA response and rapid dehydration
  • OsbZIP23, OsDT11, Overexpression of OsDT11, which encodes a novel cysteine-rich peptide, enhances drought tolerance and increases ABA concentration in rice., Moreover, the expression of OsDT11 was repressed in ABA-insensitive mutant Osbzip23 and Os2H16 RNAi lines
  • OsABF1~OsABI5~OREB1~OsbZIP10, OsbZIP23, The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice., In addition, OsABF1 directly regulates the expression of the protein phosphatase 2C (OsPP48 and OsPP108) and bZIP (OsbZIP23, OsbZIP46, and OsbZIP72) genes, thus forming a complex feedback circuit in the drought/abscisic acid signaling pathway
  • OsbZIP23, OsOTS1, Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice., We reveal that OsOTS1 SUMO protease directly targets the ABA and drought responsive transcription factor OsbZIP23 for de-SUMOylation affecting its stability
  • OsbZIP23, OsOTS1, Rice SUMO protease Overly Tolerant to Salt 1 targets the transcription factor, OsbZIP23 to promote drought tolerance in rice., OsOTS-RNAi lines show increased abundance of OsbZIP23 and increased drought responsive gene expression while OsOTS1 overexpressing lines show reduced levels of OsbZIP23 leading to suppressed drought responsive gene expression
  • OsbZIP23, OsNAC19~SNAC1~OsNAC9, Genome-Wide Identification of SNAC1-Targeted Genes Involved in Drought Response in Rice., SNAC1 can bind to the OsbZIP23 promoter, an important ABA signaling regulator, and positively regulate the expression of several ABA signaling genes
  • OsbZIP23, OsMFT2, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice., In vivo and in vitro assays showed that three bZIP transcription factors, OsbZIP23, OsbZIP66 and OsbZIP72, interacted with OsMFT2
  • OsbZIP23, OsMFT2, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice., Performance of transgenic plants demonstrated that overexpressing OsbZIP23 rescued the pre-harvest sprouting phenotype and expression decrease of ABA signaling genes caused by OsMFT2 knocking-out
  • OsbZIP23, OsbZIP72~OsABF4, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice , In vivo and in vitro assays showed that three bZIP transcription factors, OsbZIP23, OsbZIP66 and OsbZIP72, interacted with OsMFT2
  • OsbZIP23, OsMFT2, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice , In vivo and in vitro assays showed that three bZIP transcription factors, OsbZIP23, OsbZIP66 and OsbZIP72, interacted with OsMFT2
  • OsbZIP23, OsMFT2, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice , The performance of the transgenic plants demonstrated that overexpressing OsbZIP23 rescued the pre-harvest sprouting phenotype and the decrease in ABA-signaling genes expression caused by OsMFT2 knock-out
  • OsbZIP23, TRAB1~OsbZIP66, OsMFT2 is involved in the regulation of ABA signaling-mediated seed germination through interacting with OsbZIP23/66/72 in rice , In vivo and in vitro assays showed that three bZIP transcription factors, OsbZIP23, OsbZIP66 and OsbZIP72, interacted with OsMFT2
  • OsbZIP23, UGT2, Rice glycosyltransferase gene UGT2 functions in salt stress tolerance under the regulation of bZIP23 transcription factor., The transcription factor OsbZIP23 was demonstrated to regulate the UGT2 expression under stress conditions
  • OsbZIP23, UGT2, Rice glycosyltransferase gene UGT2 functions in salt stress tolerance under the regulation of bZIP23 transcription factor., Furthermore, the upstream regulatory mechanism of the UGT2 gene was identified and we found that a bZIP transcription factor, OsbZIP23, can bind to the UGT2 promoter and enhance the UGT2 transcription levels

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