- Information
- Symbol: OsLKR,SDH
- MSU: LOC_Os02g54254
- RAPdb: Os02g0783700,Os02g0783625
- Publication
- Genbank accession number
- Key message
- OsLKR/SDH was highly expressed in the aleurone and subaleurone layers of the endosperm
- Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- L), a bifunctional OsLKR/SDH is predominantly present in seeds
- Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- Connection
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- OsLKR~SDH, RPBF~OsDof3, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Here, we show that OsLKR/SDH is directly regulated by major transcriptional regulators of seed storage protein (SSP) genes: the basic leucine zipper (bZIP) transcription factor (TF), RISBZ1, and the DNA-binding with one finger (DOF) transcription factor, RPBF
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Mutation analyses in planta, trans-activation reporter assays in vivo and electrophorestic mobility shift assays in vitro showed that the RPBF-recognizing prolamin box (AAAG) and the RISBZ1-recognizing GCN4 motif (TGAG/CTCA) act as important cis-elements for proper expression of OsLKR/SDH like SSP genes
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, Reducing either RISBZ1 or RPBF decreased OsLKR/SDH levels, resulting in an increase in free lysine content in rice grain
- OsLKR~SDH, RISBZ1~OsbZIP58~OsSMF1, Differences in transcriptional regulatory mechanisms functioning for free lysine content and seed storage protein accumulation in rice grain, This result was in contrast to the fact that a significant reduction of SSP was observed only when these transcription factors were simultaneously reduced, suggesting that RISBZ1 and RPBF regulate SSP genes and OsLKR/SDH with high and limited redundancy, respectively
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