- Information
- Symbol: Lsi2,OsLsi2
- MSU: LOC_Os03g01700
- RAPdb: Os03g0107300
- Publication
- The role of the rice aquaporin Lsi1 in arsenite efflux from roots, 2010, New Phytol.
- An efflux transporter of silicon in rice, 2007, Nature.
- Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, 2007, Plant Physiol.
- The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, 2009, Plant Physiol.
- Transporters of arsenite in rice and their role in arsenic accumulation in rice grain, 2008, Proc Natl Acad Sci U S A.
- High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., 2016, Plant Cell Physiol.
- Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., 2019, Plant Cell Physiol.
- Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice, 2020, Plant Physiol Biochem.
- Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice., 2022, Plant Cell Physiol.
- Genbank accession number
- Key message
- Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots
- Furthermore, in the rice mutants defective in the silicon efflux transporter Lsi2, arsenite transport to the xylem and accumulation in shoots and grain decreased greatly
- Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier)
- The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath
- Expression of Lsi2 in Xenopus oocytes did not result in influx transport activity for silicon, but preloading of the oocytes with silicon resulted in a release of silicon, indicating that Lsi2 is a silicon efflux transporter
- By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium
- Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1
- Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1
- There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si
- The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice
- In summary, our results reveal that Si deposition at root endodermis and its resultant reduction of Na+ bypass flow is modulated by OsLsi1 and OsLsi2 and regulated by the expression of OsLsi1 and OsLsi2, implying that root Si deposition could be an active and physiologically-regulated process in rice
- In this study, we investigated the roles of OsLsi1 and OsLsi2 in Si-induced reduction of bypass flow and its resultant alleviation of salt stress by using lsi1 and lsi2 mutants (defective in OsLsi1 and OsLsi2, respectively) and their wild types (WTs)
- The polar localization of both OsLsi1 and OsLsi2 was not altered by Si supply, but their protein abundance was reduced
- The polar localization of OsLsi1 and OsLsi2 was not altered by inhibition of clathrin-mediated endocytosis (CME) by dominant-negative induction of dynamin-related protein1A and knockout of mu subunit of adaptor protein 2 complex, although the knockout mutants of OsAP2M gene showed dwarf phenotype
- Connection
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, An efflux transporter of silicon in rice, Here we describe a previously uncharacterized gene, low silicon rice 2 (Lsi2), which has no similarity to Lsi1
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, An efflux transporter of silicon in rice, The protein encoded by this gene is localized, like Lsi1, on the plasma membrane of cells in both the exodermis and the endodermis, but in contrast to Lsi1, which is localized on the distal side, Lsi2 is localized on the proximal side of the same cells
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Genotypic difference in silicon uptake and expression of silicon transporter genes in rice, Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, Recent studies have shown that arsenite is taken up by rice (Oryza sativa) roots through two silicon transporters, Lsi1 (the aquaporin NIP2;1) and Lsi2 (an efflux carrier)
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, The rice aquaporin Lsi1 mediates uptake of methylated arsenic species, The rice lsi1 mutant lost about 80% and 50% of the uptake capacity for MMA(V) and DMA(V), respectively, compared with the wild-type rice, whereas Lsi2 mutation had little effect
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Transporters of arsenite in rice and their role in arsenic accumulation in rice grain, Mutation in Lsi2 had a much greater impact on arsenic accumulation in shoots and grain in field-grown rice than Lsi1
- Lsi2~OsLsi2, Lsi3, Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice., We found that three transporters (Lsi2, Lsi3, and Lsi6) located at the node are involved in the intervascular transfer, which is required for the preferential distribution of Si
- Lsi2~OsLsi2, Lsi3, Orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice., Similar to Lsi6, knockout of Lsi2 and Lsi3 also resulted in decreased distribution of Si to the panicles but increased Si to the flag leaf
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Reduced arsenic accumulation in rice Oryza sativa L. shoot involves sulfur mediated improved thiol metabolism, antioxidant system and altered arsenic transporters., High S treatment also reduced the expression of OsLsi1 and OsLsi2, the potent transporters of As in rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High silicon accumulation in the shoot is required for down-regulating the expression of Si transporter genes in rice., A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half roots were exposed to Si
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., The efficient uptake of Si in rice is mediated by two transporters OsLsi1 and OsLsi2, which function as influx and efflux transporters, respectively
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., Herein we investigated the mechanism underlying regulation of OsLsi1 and OsLsi2 expression
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., There was a negative correlation between the expression level of OsLsi1 and OsLsi2 and shoot Si accumulation when the rice seedlings were exposed to different Si supply conditions
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, High Silicon Accumulation in the Shoot is Required for Down-Regulating the Expression of Si Transporter Genes in Rice., A split root experiment showed that the expression of both OsLsi1 and OsLsi2 was also down-regulated in half the roots without direct Si exposure when the other half of the roots were exposed to Si
- Lsi2~OsLsi2, MID1~OsARM1, OsARM1, an R2R3 MYB Transcription Factor, Is Involved in Regulation of the Response to Arsenic Stress in Rice., Chromatin immunoprecipitation-quantitative PCR of OsARM1-OEs indicated that OsARM1 binds to the conserved MYB-binding sites in the promoters or genomic regions of OsLsi1, OsLsi2, and OsLsi6 in rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- Lsi2~OsLsi2, OsNRAMP5, Altered root structure affects both expression and cellular localization of transporters for mineral element uptake in rice., The expression level of some transporter genes including OsLsi1 and OsLsi2 for Si uptake and OsNramp5 for Mn uptake was significantly decreased in the mutant compared with the wild-type rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice, In this study, we investigated the roles of OsLsi1 and OsLsi2 in Si-induced reduction of bypass flow and its resultant alleviation of salt stress by using lsi1 and lsi2 mutants (defective in OsLsi1 and OsLsi2, respectively) and their wild types (WTs)
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice, Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice, Moreover, results obtained from root split experiment using lsi1 WT showed down-regulated expression of Si transport genes (OsLsi1 and OsLsi2) in root accelerated Si deposition at root endodermis
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Root silicon deposition and its resultant reduction of sodium bypass flow is modulated by OsLsi1 and OsLsi2 in rice, In summary, our results reveal that Si deposition at root endodermis and its resultant reduction of Na+ bypass flow is modulated by OsLsi1 and OsLsi2 and regulated by the expression of OsLsi1 and OsLsi2, implying that root Si deposition could be an active and physiologically-regulated process in rice
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice., The polar localization of both OsLsi1 and OsLsi2 was not altered by Si supply, but their protein abundance was reduced
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice., Double immunostaining showed that localization of OsLsi1 and OsLsi2 was separated at the edge of the lateral polar domain by Casparian strips in the endodermis, whereas they were slightly overlapped at the transversal side of the exodermis
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Cell-Type-Dependent but CME-Independent Polar Localization of Silicon Transporters in Rice., The polar localization of OsLsi1 and OsLsi2 was not altered by inhibition of clathrin-mediated endocytosis (CME) by dominant-negative induction of dynamin-related protein1A and knockout of mu subunit of adaptor protein 2 complex, although the knockout mutants of OsAP2M gene showed dwarf phenotype
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, Dark Side of Ammonium Nitrogen in Paddy Soil with Low Organic Matter: Stimulation of Microbial AsV Reduction and AsIII Transfer from Soil to Rice Grains., Furthermore, the relative expression of As transporter genes in rice roots, such as OsLsi1, OsLsi2, and OsLsi3, was upregulated, and the translocation efficiency of As(III) from rice roots to brown rice was promoted
- Lsi1~OsNIP2;1~OsLsi1, Lsi2~OsLsi2, and N-termini as well as positively charged residues., The high Si accumulation is achieved by co-operation of two Si transporters, LOW SILICON 1 (OsLsi1) and OsLsi2, which are polarly localized in cells of the root exodermis and endodermis
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