- Information
- Symbol: EXPLA1
- MSU: LOC_Os03g04020
- RAPdb: Os03g0132200
- Publication
- GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., 2018, Int J Mol Sci.
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Genbank accession number
- Key message
- These results suggest that GW2 negatively regulates seed size by targeting EXPLA1 for degradation through its E3 ubiquitin ligase activity
- GW2 and EXPLA1 colocalized to the nucleus when expressed simultaneously
- Connection
- EXPLA1, GW2~OsGW2, GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., Here, we show that rice Grain Width 2 (GW2), a RING-type E3 ubiquitin ligase, can control seed development by catalyzing the ubiquitination of expansin-like 1 (EXPLA1), a cell wall-loosening protein that increases cell growth
- EXPLA1, GW2~OsGW2, GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., Yeast two-hybrid and in vitro pull-down assays showed a strong interaction between GW2 and EXPLA1
- EXPLA1, GW2~OsGW2, GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., In vitro ubiquitination analysis demonstrated that EXPLA1 was ubiquitinated by GW2 at lysine 279 (K279)
- EXPLA1, GW2~OsGW2, GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., GW2 and EXPLA1 colocalized to the nucleus when expressed simultaneously
- EXPLA1, GW2~OsGW2, GW2 Functions as an E3 Ubiquitin Ligase for Rice Expansin-Like 1., These results suggest that GW2 negatively regulates seed size by targeting EXPLA1 for degradation through its E3 ubiquitin ligase activity
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