- Information
- Symbol: OsDof12,OsCDF1
- MSU: LOC_Os03g07360
- RAPdb: Os03g0169600
- Publication
- Functional characterization of rice OsDof12, 2009, Planta.
- OsMADS50 and OsMADS56 function antagonistically in regulating long day LD-dependent flowering in rice, 2009, Plant Cell Environ.
- Sense and antisense OsDof12 transcripts in rice, 2008, BMC Mol Biol.
- Overexpression of OsDof12 affects plant architecture in rice Oryza sativa L.., 2015, Front Plant Sci.
- Genbank accession number
- Key message
- The transgenic lines overexpressing OsDof12 showed early flowering under long-day (LD) conditions, whereas OsDof12 overexpression had no effect on flowering time under short-day (SD) conditions
- These results suggested that OsDof12 might regulate flowering by controlling the expression of Hd3a and OsMADS14
- Here, we further investigated the other important agronomical traits of the transgenic plants overexpressing OsDof12 and found that overexpressing OsDof12 could lead to reduced plant height, erected leaf, shortened leaf blade, and smaller panicle resulted from decreased primary and secondary branches number
- Overexpression of OsDof12 affects plant architecture in rice (Oryza sativa L.).
- These results implied that OsDof12 is involved in rice plant architecture formation
- Taken together, our results suggested that overexpression of OsDof12 could lead to altered plant architecture by suppressing BR signaling
- Thus, OsDof12 might be used as a new potential genetic regulator for future rice molecular breeding
- Of note, in WT plants the expression of OsDof12 was found up-regulated by BR treatment while in OsDof12 overexpression plants two positive BR signaling regulators, OsBRI1 and OsBZR1, were significantly down-regulated, indicating that OsDof12 may act as a negative BR regulator in rice
- Furthermore, we performed a series of Brassinosteroid (BR)-responsive tests and found that overexpression of OsDof12 could also result in BR hyposensitivity
- Connection
- OsDof12~OsCDF1, OsMADS51~OsMADS65, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- Ehd1, OsDof12~OsCDF1, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- OsDof12~OsCDF1, OsMADS14, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- OsDof12~OsCDF1, OsMADS14, Functional characterization of rice OsDof12, These results suggested that OsDof12 might regulate flowering by controlling the expression of Hd3a and OsMADS14
- Hd3a, OsDof12~OsCDF1, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- Hd3a, OsDof12~OsCDF1, Functional characterization of rice OsDof12, These results suggested that OsDof12 might regulate flowering by controlling the expression of Hd3a and OsMADS14
- Hd1, OsDof12~OsCDF1, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- OsDof12~OsCDF1, OsGI, Functional characterization of rice OsDof12, In transgenic lines overexpressing OsDof12, the transcription levels of Hd3a and OsMADS14 were up-regulated under LD conditions but not SD conditions, whereas the expression of Hd1, OsMADS51, Ehd1 and OsGI did not change under LD and SD conditions
- Ghd7, OsDof12~OsCDF1, OsMADS50 and OsMADS56 function antagonistically in regulating long day LD-dependent flowering in rice, On the other hand, mRNA levels of OsGI, Hd1, OsId1, OsDof12, Ghd7, Hd6 and SE5 were unchanged
- Hd1, OsDof12~OsCDF1, OsMADS50 and OsMADS56 function antagonistically in regulating long day LD-dependent flowering in rice, On the other hand, mRNA levels of OsGI, Hd1, OsId1, OsDof12, Ghd7, Hd6 and SE5 were unchanged
- Hd6~CK2, OsDof12~OsCDF1, OsMADS50 and OsMADS56 function antagonistically in regulating long day LD-dependent flowering in rice, On the other hand, mRNA levels of OsGI, Hd1, OsId1, OsDof12, Ghd7, Hd6 and SE5 were unchanged
- OsDof12~OsCDF1, OsGI, OsMADS50 and OsMADS56 function antagonistically in regulating long day LD-dependent flowering in rice, On the other hand, mRNA levels of OsGI, Hd1, OsId1, OsDof12, Ghd7, Hd6 and SE5 were unchanged
- FKF1~OsFKF1, OsDof12~OsCDF1, Rice FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 OsFKF1 promotes flowering independent of photoperiod., Like Arabidopsis FKF1, which interacts with GI and CDF1, OsFKF1 also interacts with OsGI and OsCDF1 (also termed OsDOF12)
- D61~OsBRI1~OsBRKq1~HFR131, OsDof12~OsCDF1, Overexpression of OsDof12 affects plant architecture in rice Oryza sativa L.., Of note, in WT plants the expression of OsDof12 was found up-regulated by BR treatment while in OsDof12 overexpression plants two positive BR signaling regulators, OsBRI1 and OsBZR1, were significantly down-regulated, indicating that OsDof12 may act as a negative BR regulator in rice
- OsBZR1~BZR1, OsDof12~OsCDF1, Overexpression of OsDof12 affects plant architecture in rice Oryza sativa L.., Of note, in WT plants the expression of OsDof12 was found up-regulated by BR treatment while in OsDof12 overexpression plants two positive BR signaling regulators, OsBRI1 and OsBZR1, were significantly down-regulated, indicating that OsDof12 may act as a negative BR regulator in rice
- OsDof12~OsCDF1, OsDof4, Constitutive expression of OsDof4, encoding a C2-C2 zinc finger transcription factor, confesses its distinct flowering effects under long- and short-day photoperiods in rice Oryza sativa L.., Phylogenic analysis indicated that OsDof4 is closely clustered into the same subgroup with CDFs and OsDof12
- OsDof12~OsCDF1, OsDof12os, Sense and antisense OsDof12 transcripts in rice., At different developmental stages in rice, the OsDof12 and OsDof12os transcripts exhibited reciprocal expression patterns
- OsDof12~OsCDF1, OsDof12os, Sense and antisense OsDof12 transcripts in rice., In the ProOsDof12-GUS and ProOsDof12os-GUS transgenic rice plants, the expression profiles of GUS were consistent with those of the OsDof12 and OsDof12os transcripts, respectively
- OsDof12~OsCDF1, OsDof12os, Sense and antisense OsDof12 transcripts in rice., CONCLUSION: Based on the expression profiles of OsDof12 and OsDof12os, the expression patterns of GUS in the ProOsDof12-GUS and ProOsDof12os-GUS transgenic rice plants and the predicted common cis-regulatory elements shared by the two promoters, we suggest that the co-expression patterns of OsDof12 and OsDof12os might be attributed to the basically common nature of the two promoters
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