- Information
- Symbol: OsINO1-1,OsINO1
- MSU: LOC_Os03g09250
- RAPdb: Os03g0192700
- Publication
- Identification and organization of chloroplastic and cytosolic L-myo-inositol 1-phosphate synthase coding genes in Oryza sativa: comparison with the wild halophytic rice, Porteresia coarctata, 2010, Planta.
- An insight into the molecular basis of salt tolerance of L-myo-inositol 1-P synthase PcINO1 from Porteresia coarctata Roxb. Tateoka, a halophytic wild rice, 2006, Plant Physiol.
- Genbank accession number
- Key message
- A 37-amino acid stretch between Trp-174 and Ser-210 has been confirmed as the salt-tolerance determinant domain in PcINO1 both by loss or gain of salt tolerance by either deletion or by addition to salt-sensitive MIPS(s) of Oryza (OsINO1) and Brassica juncea (BjINO1)
- 4,4’-Dianilino-1,1’-binaphthyl-5,5-disulfonic acid binding experiments revealed a lower hydrophobic surface on PcINO1 than OsINO1, contributed by this 37-amino acid stretch explaining the differential behavior of OsINO1 and PcINO1 both with respect to their enzymatic functions and thermodynamic stability in high salt environment
- 4) has been identified by matrix-assisted laser desorption time-of-flight mass spectrometry analysis of the purified and immunologically cross-reactive approximately 60 kDa chloroplastic protein following two-dimensional polyacrylamide gel electrophoresis, which exhibited sequence identity with the cytosolic MIPS coded by OsINO1-1 gene
- A possible chloroplastic transit peptide sequence was identified upstream of the OsINO1-1 gene upon analysis of rice genome
- Bioinformatic analysis mapped the chloroplastic MIPS (OsINO1-1) gene on chromosome 3, and a second MIPS gene (OsINO1-2) on chromosome 10 which lacks conventional chloroplast transit peptide sequence as in OsINO1-1
- Connection
- OsINO1-1~OsINO1, OsINO1-2, Identification and organization of chloroplastic and cytosolic L-myo-inositol 1-phosphate synthase coding genes in Oryza sativa: comparison with the wild halophytic rice, Porteresia coarctata, Bioinformatic analysis mapped the chloroplastic MIPS (OsINO1-1) gene on chromosome 3, and a second MIPS gene (OsINO1-2) on chromosome 10 which lacks conventional chloroplast transit peptide sequence as in OsINO1-1
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