OsLG3b,OsMADS1,LHS1,AFO

2015-01-20 | Categories genes  | Tags meristem  palea  fertility  leaf  spikelet  branching  heading date  transcription factor  sterile  inflorescence  floral  flower  cytokinin  spikelet meristem  stamen  lemma  panicle  architecture  auxin  reproductive  floral meristem  growth  vegetative  seed  spikelet development  development  floral organ  flower development  organ identity  R protein  floral meristem determinacy  grain  domestication  grain length  yield  breeding  seed development  grain size  map-based cloning  quality  grain shape  ovule  homologous recombination 

• Information
  • Symbol: OsLG3b,OsMADS1,LHS1,AFO
  • MSU: LOC_Os03g11614
  • RAPdb: Os03g0215400
• Publication
  • Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice, 2006, Planta.
  • MADS-box gene family in rice: genome-wide identification, organization and expression profiling during reproductive development and stress, 2007, BMC Genomics.
  • Rice LHS1/OsMADS1 controls floret meristem specification by coordinated regulation of transcription factors and hormone signaling pathways, 2013, Plant Physiol.
  • Intragenic control of expression of a rice MADS box gene OsMADS1, 2008, Mol Cells.
  • The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice, 2009, Cell Res.
  • NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice, 2009, Cell Res.
  • Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, 2004, Plant J.
  • OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, 2005, Plant J.
  • Functional conservation and diversification of class E floral homeotic genes in rice Oryza sativa, 2010, Plant J.
  • OsLEC1/OsHAP3E participates in the determination of meristem identity in both vegetative and reproductive developments of rice, 2013, J Integr Plant Biol.
  • DEP and AFO regulate reproductive habit in rice, 2010, PLoS Genet.
  • A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice, 2009, Plant J.
  • leafy hull sterile1 Is a Homeotic Mutation in a Rice MADS Box Gene Affecting Rice Flower Development, 2000, The Plant Cell Online.
  • Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, 2011, Plant Cell Physiol.
  • The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development, 2010, Plant Physiol.
  • Ectopic expression of rice OsMADS1 reveals a role in specifying the lemma and palea, grass floral organs analogous to sepals, 2001, Dev Genes Evol.
  • Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene, 1994, Plant Mol Biol.
  • Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene, 2005, Plant Mol Biol.
  • Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development., 2015, Mol Plant.
  • Genome-wide targets regulated by the OsMADS1 transcription factor reveals its DNA recognition properties., 2016, Plant Physiol.
  • OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice., 2016, Front Plant Sci.
  • Alternative<U+00A0>splicing of OsLG3b controls grain length and yield in japonica rice., 2018, Plant Biotechnol J.
  • G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice., 2018, Nat Commun.
  • Rice shoot and floral meristem development: An overview of developmental regulators of meristem maintenance and organ identity., 2019, J Exp Bot.
  • [Characterization of the ‘Oat-Like Rice’ Caused by a Novel Allele OsMADS1 Olr Reveals Vital Importance of OsMADS1 in Regulating Grain Shape in Oryza sativa L](http://www.ncbi.nlm.nih.gov/pubmed?term=Characterization of the ‘Oat-Like Rice’ Caused by a Novel Allele OsMADS1 Olr Reveals Vital Importance of OsMADS1 in Regulating Grain Shape in Oryza sativa L%5BTitle%5D), 2020, Rice (N Y).
  • OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, 2020, J Exp Bot.
  • Bin-based genome-wide association studies reveal superior alleles for improvement of appearance quality using a 4-way MAGIC population in rice, 2020, J Adv Res.
  • Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, 2021, Planta.
• Genbank accession number
  • AK069728
  • L34271
  • AK069728
  • L34271
• Key message
  • OsMADS1 is not expressed during panicle branching; earliest expression is in spikelet meristems where it is excluded from the outer rudimentary/sterile glumes
  • OsMADS1 knockdown perturbs the differentiation of specific cell types in the lemma and palea, creating glume-like features, with severe derangements in lemma differentiation
  • We thus demonstrate the differential contribution of OsMADS1 for lemma versus palea development and provide evidence for its regulatory function in patterning inner whorl organs
  • OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs
  • Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility
  • Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs
  • Collectively, our study suggests that the origin and diversification of OsMADS34 and OsMADS1 contribute to the origin of distinct grass inflorescences and spikelets
  • The overexpression of OsMGH3 during the vegetative phase affects the overall plant architecture, whereas its inflorescence-specific overexpression creates short panicles with reduced branching, resembling in part the effects of OsMADS1 overexpression
  • During organogenesis, OsMADS1 expression is confined to the lemma and palea, with weak expression in the carpel
  • Together, these data suggest a distinct role for OsMADS1 and its monocot relatives in assigning lemma/palea identity
  • Ectopic expression of rice OsMADS1 reveals a role in specifying the lemma and palea, grass floral organs analogous to sepals
  • RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsMGH3/OsGH3-8 is expressed abundantly in rice florets and is regulated by two related and redundant transcription factors, OsMADS1 and OsMADS6, but its contribution to flower development is not known
  • These characteristics are very similar to those of leafy hull sterile1 (lhs1)
  • And molecular analysis indicated that nsr was a novel homeotic mutation in OsMADS1, suggesting that OsMADS1 played a distinct role in regulating the differentiation pattern of floral primordium and in conferring the determination of flower meristem
  • The multiple effects of OsMADS1 in promoting auxin transport, signaling, and auxin-dependent expression and its direct repression of three cytokinin A-type response regulators show its role in balancing meristem growth, lateral organ differentiation, and determinacy
  • leafy hull sterile1/OsMADS1, from a grass-specific subgroup of LOFSEP genes, is required for specifying a single floret on the spikelet meristem and for floret organ development, but its downstream mechanisms are unknown
  • Severe loss-of-function mutations of OsMADS1cause complete homeotic conversion of organs (lodicules, stamens, and carpels) of three inner whorls into lemma- and palea-like structures
  • The gain-of-function of OsMADS1 transgenic lines presented the transformation of outer glumes to lemma-/palea-like organs and no changes in length of lemma and palea, but loss-of-function of OsMADS1 transgenic lines displayed the overdeveloped lemma and palea
  • Both findings revealed that OsMADS1 played a role in specifying lemma and palea and acted as a repressor of overdevelopment of lemma and palea
  • Ectopic OsMADS1 expression results in stunted panicles with irregularly positioned branches and spikelets
  • Strikingly, mutation of a SEPALLATA (SEP)-like gene, OsMADS1 (LHS1), enhanced the defect of osmads6 flowers, and no inner floral organs or glume-like structures were observed in whorls 2 and 3 of osmads1-z osmads6-1 flowers
  • Furthermore, the osmads1-z osmads6-1 double mutants developed severely indeterminate floral meristems
  • Our finding, therefore, suggests that the ancient OsMADS6 gene is able to specify floral state by determining floral organ and meristem identities in monocot crop rice together with OsMADS1
  • In this work, we found three naturally occurring mutants in rice, namely, phoenix (pho), degenerative palea (dep), and abnormal floral organs (afo)
  • 9-kb 5’ upstream promoter region, are required for the GUS expression pattern that coincides with flower-preferential expression of OsMADS1
  • Notably, incorporation of the intragenic region into the CaMV35S promoter directed the GUS expression pattern similar to that of the endogenous spatial expression of OsMADS1 in flowers
  • Conversely, ectopic OsMADS1 expression suffices to direct lemma-like differentiation in the glume
  • Moreover, analysis of the double mutant osmads34 osmads1 suggests that OsMADS34 specifies the identities of floral organs, including the lemma/palea, lodicules, stamens, and carpel, in combination with another rice SEP-like gene, OsMADS1
  • We also found that the floral meristem and organ identity gene OsLHS1 showed altered expression with respect to both pattern and levels in the eg1 mutant, and is probably responsible for the pleiotropic floral defects in eg1
  • Rice LHS1/OsMADS1 controls floret meristem specification by coordinated regulation of transcription factors and hormone signaling pathways
  • Thus, OsMADS1 and OsMADS15 are both required to ensure sexual reproduction in rice and mutations of them lead to the switch of reproductive habit from sexual to asexual in rice
  • These results suggest that the OsMADS1 gene is involved in flower induction and that it may be used for genetic manipulation of certain plant species
  • Furthermore, the expression of two regulators of flowering, Hd3a and OsMADS1, was also affected in the nl1 mutant
  • These phenotypes resemble the phenotypes caused by mutations of the dicot E-class genes, such as the Arabidopsis SEP123(SEPALLATA1/2/3) and the petunia FBP2(Floral Binding Protein 2), suggesting that OsMADS1play a very similar role in rice to that of defined E-class genes in dicot plants
  • Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene
  • OsMADS1 is a rice MADS box gene necessary for floral development
  • Through a screen for OsMADS1 targets we identify a flower-specific Nt-gh3 type gene, OsMGH3, as a downstream gene
  • It has been demonstrated previously that one of these genes, OsMADS1 (for Oryza sativa MADS box gene1), is expressed preferentially in flowers and causes early flowering when ectopically expressed in tobacco plants
  • In this study, we demonstrated that ectopic expression of OsMADS1 in rice also results in early flowering
  • To further investigate the role of OsMADS1 during rice flower development, we generated transgenic rice plants expressing altered OsMADS1 genes that contain missense mutations in the MADS domain
  • DEP and AFO regulate reproductive habit in rice
  • Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice
  • OsJAZ1 also interacts with OsMYC2, a transcription factor in the JA signalling pathway, and represses OsMYC2’s role in activating OsMADS1, an E-class gene crucial to the spikelet development.
  • Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development.
  • However, molecular mechanisms underlying interactions of OsMADS1 with other floral homeotic genes in regulating flower development remains largely elusive
  • The physical and genetic interaction of OsMADS1 and OsMADS3 is essential for floral meristem activity maintenance and organ identity specification; while OsMADS1 physically and genetically interacts with OsMADS58 in regulating floral meristem determinacy and suppressing spikelet meristem reversion
  • Gene expression profiling further identified that OsMADS1 regulates expression of OsMADS17, and affects other genes involved in floral identity and hormone signaling
  • In brief, this work provides new insights about the physical and regulatory interaction network of OsMADS1 with other floral homeotic genes in rice floral organ identity specification and meristem determinacy
  • In this work, we studied the genetic interactions of OsMADS1 with B-, C-, and D-class genes along with physical interactions among their proteins, and provided some important evidence to further support the neofunctionalization of two rice C-class genes
  • OsMADS1 controls rice (Oryza sativa) floral fate and organ development
  • Overall, OsMADS1 binds to several regulatory genes and, probably in combination with other factors, controls a gene regulatory network that ensures rice floret development
  • Genome-wide targets regulated by the OsMADS1 transcription factor reveals its DNA recognition properties.
  • Combining expression data from OsMADS1 knockdown florets with these DNA binding data, a snapshot of a gene regulatory network was deduced where targets, such as AP2/ERF and bHLH transcription factors and chromatin remodelers form nodes
  • OsMADS1 specifies the determinacy of spikelet meristem and lemma/palea identity in rice
  • OsMADS1 Represses microRNA172 in Elongation of Palea/Lemma Development in Rice.
  • However, the pathway through which OsMADS1 regulates floral organs remains elusive; here, we identified the microRNA172 (miR172) family as possible regulators downstream of OsMADS1
  • Our results suggested that in rice, OsMADS1 and miR172s/AP2s formed a regulatory network involved in floral organ development, particularly the elongation of the lemma and the palea
  • Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages
  • Alternative<U+00A0>splicing of OsLG3b controls grain length and yield in japonica rice.
  • Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region
  • Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica
  • Analysis of haplotypes and introgression regions revealed that the long-grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection
  • OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice
  • The G subunits GS3 and DEP1 interact directly with the conserved keratin-like domain of MADS transcription factors, function as cofactors to enhance OsMADS1 transcriptional activity and promote the co-operative transactivation of common target genes, thereby regulating grain size and shape
  • Yet, several evidences suggest the evolution of distinctive functions for some of these rice floral regulators, the occurrence of other grass species-specific factors and regulatory pathways - for example LOFSEP ‘E’ class genes OsMADS1 and OsMAD34 and ramosa genes
  • Further linkage analysis indicated that the point mutation in the OsMADS1Olr is associated with Oat-like rice phenotype, and expression analysis of the OsMADS1 by qRT-PCR and GUS staining also indicated that it is highly expressed in flower organs as well as in the early stages of grain development
  • Finally, compared with Oat-like rice, OsMADS1Olr-overexpressing and OsMADS1-RNAi plants, mild phenotype of seed-specific OsMADS1-RNAi transgenic plants indicated that OsMADS1 may has has a direct regulation role in grain development and the grain phenotypes of Oat-like rice, OsMADS1Olr-overexpressing and OsMADS1-RNAi plants are majorly caused by the abnormal lemma and palea development
  • Characterization of the ‘Oat-Like Rice’ Caused by a Novel Allele OsMADS1 Olr Reveals Vital Importance of OsMADS1 in Regulating Grain Shape in Oryza sativa L
  • Furthermore, our findings suggested that OsMADS1 mediates grain shape possibly by affecting the expression of representative genes related to grain shape regulation
  • Thus, this study not only revealed that OsMADS1 plays a vital role in regulating grain shape of rice but also highlighted the importance and value of OsMADS1 to improve the quality and yield of rice by molecular breeding
  • By map-based cloning, we discovered that Oat-like rice harbors a novel allele of OsMADS1 gene (OsMADS1Olr), which has a spontaneous point mutation that causes the substitution of an amino acid that is highly conserved in the MADS-box domain of the MADS-box family
  • A new, stable, null mutant of OsMADS1 generated by homologous recombination-based gene targeting in an indica rice confirms its regulatory role for floral meristem identity, its determinate development and floral organ differentiation
  • Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • We also noticed striking instances of floral reversion to inflorescence and vegetative states which has not been reported for other mutant alleles of OsMADS1 and further reinforces the role of OsMADS1 in controlling floral meristem determinacy
  • Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting
  • In this study, we generated an OsMADS1 null mutant by homologous recombination-mediated gene targeting by inserting a selectable marker gene (hpt) in OsMADS1 and replacing parts of its cis-regulatory and coding sequences
• Connection
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS16~SPW1, Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice, Moreover, it was indicated that OsMADS1 upregulated the transcript level of AP3 homologue OsMADS16, using real-time PCR analysis on gain- and loss-of-function of OsMADS1 transgenic lines
  • OsETT2~OsETTIN2, OsLG3b~OsMADS1~LHS1~AFO, Rice LHS1/OsMADS1 controls floret meristem specification by coordinated regulation of transcription factors and hormone signaling pathways, We found that the OsMADS1 targets OsETTIN1 and OsETTIN2 redundantly ensure carpel differentiation
  • OsETTIN1~OsARF15, OsLG3b~OsMADS1~LHS1~AFO, Rice LHS1/OsMADS1 controls floret meristem specification by coordinated regulation of transcription factors and hormone signaling pathways, We found that the OsMADS1 targets OsETTIN1 and OsETTIN2 redundantly ensure carpel differentiation
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS5, Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss-of-function mutants of the OsMADS1 gene, ) by characterizing tissue culture-induced mutants of two MADS-box genes, OsMADS1and OsMADS5, which form a subclade within the well-supported clade of SEP-genes (E-function) phylogeny
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice, Strikingly, mutation of a SEPALLATA (SEP)-like gene, OsMADS1 (LHS1), enhanced the defect of osmads6 flowers, and no inner floral organs or glume-like structures were observed in whorls 2 and 3 of osmads1-z osmads6-1 flowers
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice, Furthermore, the osmads1-z osmads6-1 double mutants developed severely indeterminate floral meristems
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, The AGL6-like gene OsMADS6 regulates floral organ and meristem identities in rice, Our finding, therefore, suggests that the ancient OsMADS6 gene is able to specify “floral state” by determining floral organ and meristem identities in monocot crop rice together with OsMADS1
  • Hd3a, OsLG3b~OsMADS1~LHS1~AFO, NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice, Furthermore, the expression of two regulators of flowering, Hd3a and OsMADS1, was also affected in the nl1 mutant
  • NL1~SNFL1, OsLG3b~OsMADS1~LHS1~AFO, NECK LEAF 1, a GATA type transcription factor, modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice, Furthermore, the expression of two regulators of flowering, Hd3a and OsMADS1, was also affected in the nl1 mutant
  • Hd1, OsLG3b~OsMADS1~LHS1~AFO, Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS18, Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS50~OsSOC1~DTH3, Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS14, Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 SOC1/AGL20 ortholog in rice, RT-PCR analyses of the OsMADS50 KO and ubiquitin (ubi):OsMADS50 plants showed that OsMADS50 is an upstream regulator of OsMADS1, OsMADS14, OsMADS15, OsMADS18, and Hd (Heading date)3a, but works either parallel with or downstream of Hd1 and O
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, Through a screen for OsMADS1 targets we identify a flower-specific Nt-gh3 type gene, OsMGH3, as a downstream gene
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, The delayed transcription activation of OsMGH3 by dexamethasone-inducible OsMADS1 suggests indirect activation
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, The OsMGH3 floret expression profile suggests a novel role for OsMADS1 as an early-acting regulator of second and third whorl organ fate
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS7~OsMADS45, Functional conservation and diversification of class E floral homeotic genes in rice Oryza sativa, Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS8~OsMADS24, Functional conservation and diversification of class E floral homeotic genes in rice Oryza sativa, Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS5, Functional conservation and diversification of class E floral homeotic genes in rice Oryza sativa, Simultaneous knockdown of the four rice SEP-like genes OsMADS1, OsMADS5, OsMADS7 and OsMADS8, leads to homeotic transformation of all floral organs except the lemma into leaf-like organs
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, DEP and AFO regulate reproductive habit in rice, Further map-based cloning and microarray analysis revealed that dep mutant was caused by a genetic alteration in OsMADS15 while afo was caused by an epigenetic mutation in OsMADS1
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, DEP and AFO regulate reproductive habit in rice, Thus, OsMADS1 and OsMADS15 are both required to ensure sexual reproduction in rice and mutations of them lead to the switch of reproductive habit from sexual to asexual in rice
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, DEP and AFO regulate reproductive habit in rice, In this work, we found three naturally occurring mutants in rice, namely, phoenix (pho), degenerative palea (dep), and abnormal floral organs (afo)
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, DEP and AFO regulate reproductive habit in rice, Genetic analysis of them indicated that the stable pseudovivipary mutant pho was a double mutant containing both a Mendelian mutation in DEP and a non-Mendelian mutation in AFO
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, DEP and AFO regulate reproductive habit in rice, DEP and AFO regulate reproductive habit in rice
  • EG1~GY1, OsLG3b~OsMADS1~LHS1~AFO, A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice, We also found that the floral meristem and organ identity gene OsLHS1 showed altered expression with respect to both pattern and levels in the eg1 mutant, and is probably responsible for the pleiotropic floral defects in eg1
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, OsMGH3/OsGH3-8 is expressed abundantly in rice florets and is regulated by two related and redundant transcription factors, OsMADS1 and OsMADS6, but its contribution to flower development is not known
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, We functionally characterize OsMGH3 by overexpression and knock-down analysis and show a partial overlap in these phenotypes with that of mutants in OsMADS1 and OsMADS6
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, The overexpression of OsMGH3 during the vegetative phase affects the overall plant architecture, whereas its inflorescence-specific overexpression creates short panicles with reduced branching, resembling in part the effects of OsMADS1 overexpression
  • OsGH3.8~OsGH3-8~OsMGH3, OsLG3b~OsMADS1~LHS1~AFO, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, OsMGH3/OsGH3-8 is expressed abundantly in rice florets and is regulated by two related and redundant transcription factors, OsMADS1 and OsMADS6, but its contribution to flower development is not known
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, We functionally characterize OsMGH3 by overexpression and knock-down analysis and show a partial overlap in these phenotypes with that of mutants in OsMADS1 and OsMADS6
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility, Auxin-responsive OsMGH3, a common downstream target of OsMADS1 and OsMADS6, controls rice floret fertility
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS34~PAP2, The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development, Moreover, analysis of the double mutant osmads34 osmads1 suggests that OsMADS34 specifies the identities of floral organs, including the lemma/palea, lodicules, stamens, and carpel, in combination with another rice SEP-like gene, OsMADS1
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS34~PAP2, The SEPALLATA-like gene OsMADS34 is required for rice inflorescence and spikelet development, Collectively, our study suggests that the origin and diversification of OsMADS34 and OsMADS1 contribute to the origin of distinct grass inflorescences and spikelets
  • OsLG3b~OsMADS1~LHS1~AFO, OsMYC2, Jasmonic acid regulates spikelet development in rice, OsJAZ1 also interacts with OsMYC2, a transcription factor in the JA signalling pathway, and represses OsMYC2’s role in activating OsMADS1, an E-class gene crucial to the spikelet development.
  • OsIG1, OsLG3b~OsMADS1~LHS1~AFO, Down-regulation of a LBD-like gene, OsIG1, leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice, Additionally, down-regulation of OsIG1 differentially affected the expression of genes associated with the floral organ development including EG1, OsMADS6 and OsMADS1.
  • OsEMF2b, OsLG3b~OsMADS1~LHS1~AFO, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type.
  • OsEMF2b, OsLG3b~OsMADS1~LHS1~AFO, The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice, OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed.
  • OsLG3b~OsMADS1~LHS1~AFO, OSMADS3, Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development., The physical and genetic interaction of OsMADS1 and OsMADS3 is essential for floral meristem activity maintenance and organ identity specification; while OsMADS1 physically and genetically interacts with OsMADS58 in regulating floral meristem determinacy and suppressing spikelet meristem reversion
  • OsLG3b~OsMADS1~LHS1~AFO, OSMADS58, Interactions of OsMADS1 with Floral Homeotic Genes in Rice Flower Development., The physical and genetic interaction of OsMADS1 and OsMADS3 is essential for floral meristem activity maintenance and organ identity specification; while OsMADS1 physically and genetically interacts with OsMADS58 in regulating floral meristem determinacy and suppressing spikelet meristem reversion
  • FZP~BFL1~SGDP7~PAA7, OsLG3b~OsMADS1~LHS1~AFO, Regulatory role of FZP in the determination of panicle branching and spikelet formation in rice., FZP overexpression positively regulates the expression of a subset of the class B genes, AGL6 genes (OsMADS6 and OsMADS17) as well as class E genes (OsMADS1, OsMADS7 and OsMADS8) in floral meristem (FM)
  • EG1~GY1, OsLG3b~OsMADS1~LHS1~AFO, A High Temperature-Dependent Mitochondrial Lipase EXTRA GLUME1 Promotes Floral Phenotypic Robustness against Temperature Fluctuation in Rice Oryza sativa L.., Furthermore, we found that numerous environmentally responsive genes including many floral identity genes are transcriptionally repressed in eg1 mutants and OsMADS1, OsMADS6 and OsG1 genetically act downstream of EG1 to maintain floral robustness
  • CFO1~OsMADS32, OsLG3b~OsMADS1~LHS1~AFO, Genome-wide targets regulated by the OsMADS1 transcription factor reveals its DNA recognition properties., We show that the expression status of these nodal factors can be altered by inducing the OsMADS1-GR fusion protein and present a model for a regulatory cascade where the direct targets of OsMADS1, OsbHLH108/SPT, OsERF034 and OsHSF24, in turn control genes such as OsMADS32 and OsYABBY5
  • OsLG3b~OsMADS1~LHS1~AFO, OsYABBY5~OsYAB3~TOB1, Genome-wide targets regulated by the OsMADS1 transcription factor reveals its DNA recognition properties., We show that the expression status of these nodal factors can be altered by inducing the OsMADS1-GR fusion protein and present a model for a regulatory cascade where the direct targets of OsMADS1, OsbHLH108/SPT, OsERF034 and OsHSF24, in turn control genes such as OsMADS32 and OsYABBY5
  • NSG~NSG1~LRG1, OsLG3b~OsMADS1~LHS1~AFO, nonstop glumes nsg, a novel mutant affects spikelet development in rice, The expression of OsMADS4, OsMADS16, DL and OsMADS3 decreased distinctly, and OsMADS1 increased in nsg panicle, suggests that NSG affected spikelet development through influencing the expression of floral hometic genes
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS5, Loss of LOFSEP transcription factor function converts Spikelet to Leaf-like Structures in Rice., OsMADS5 belongs to the conserved LOFSEP subgroup along with OsMADS1 and OsMADS34
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS5, Loss of LOFSEP transcription factor function converts Spikelet to Leaf-like Structures in Rice., Genetic and molecular analyses demonstrated that OsMADS1, OsMADS5 and OsMADS34 together regulate floral meristem determinacy, and specify the identities of spikelet organs by positively regulating the other MADS-box floral homeotic genes
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS5, Loss of LOFSEP transcription factor function converts Spikelet to Leaf-like Structures in Rice., Experiments conducted in yeast also suggested that OsMADS1, OsMADS5 and OsMADS34 form protein-protein interactions with other MADS-box floral homeotic members, which seems to be a typical, conserved feature of plant SEP proteins
  • DEP1~DN1~qPE9-1~OsDEP1, OsLG3b~OsMADS1~LHS1~AFO, G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice., The Gγ subunits GS3 and DEP1 interact directly with the conserved keratin-like domain of MADS transcription factors, function as cofactors to enhance OsMADS1 transcriptional activity and promote the co-operative transactivation of common target genes, thereby regulating grain size and shape
  • GS3, OsLG3b~OsMADS1~LHS1~AFO, G-protein βγ subunits determine grain size through interaction with MADS-domain transcription factors in rice., The Gγ subunits GS3 and DEP1 interact directly with the conserved keratin-like domain of MADS transcription factors, function as cofactors to enhance OsMADS1 transcriptional activity and promote the co-operative transactivation of common target genes, thereby regulating grain size and shape
  • OsGI-Hd1-Hd3a~RFT1, OsLG3b~OsMADS1~LHS1~AFO, Overexpression of RICE FLOWERING LOCUS T 1 RFT1 Induces Extremely Early Flowering in Rice., FRUITFULL (FUL) clade genes OsMADS14 , OsMADS1 5, and OsMADS18 were highly induced in the RFT1 -expressing meristems
  • OsGI-Hd1-Hd3a~RFT1, OsLG3b~OsMADS1~LHS1~AFO, Overexpression of RICE FLOWERING LOCUS T 1 RFT1 Induces Extremely Early Flowering in Rice., RFT1 overexpression also induced SEPALLATA (SEP)-clade genes OsMADS1 , OsMADS5 , and OsMADS7 in the greening calli before floral transition occurred
  • OsGI-Hd1-Hd3a~RFT1, OsLG3b~OsMADS1~LHS1~AFO, Overexpression of RICE FLOWERING LOCUS T 1 RFT1 Induces Extremely Early Flowering in Rice., This indicated that OsMADS14 , OsMADS1 5, OsMADS18 , and OsMADS34 act immediately downstream of RFT1
  • NSG~NSG1~LRG1, OsLG3b~OsMADS1~LHS1~AFO, NONSTOP GLUMES 1 Encoding a C2H2 Zinc Finger Protein That Regulates Spikelet Development in Rice., Furthermore, the NSG1 protein was able to bind to regulatory regions of LHS1, and then recruits the co-repressor TPRs to repress their expression by down-regulating histone acetylation levels of their chromatin
  • NSG~NSG1~LRG1, OsLG3b~OsMADS1~LHS1~AFO, NONSTOP GLUMES 1 Encoding a C2H2 Zinc Finger Protein That Regulates Spikelet Development in Rice., The results suggest that NSG1 plays a pivotal role in maintaining organ identities in the spikelet by repressing the expression of LHS1, DL, MFO1
  • OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., EMSAs and ChIP-qPCR assay confirmed that WG7 directly bound to the promoter of OsMADS1, a grain size gene, and thereby significantly activated its expression
  • OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., Compared with the wild-type, deletion mutants of the cis-element motif exhibited lower expression of OsMADS1 and produced narrower grains, implicating the requirement of this motif for WG7 function
  • OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., ChIP-qPCR assays showed that WG7 enhanced histone H3K4me3 enrichment in the promoter of OsMADS1
  • OsCW-ZF7~WG7, OsLG3b~OsMADS1~LHS1~AFO, Wide Grain 7 increases grain width by enhancing H3K4me3<U+00A0>enrichment in the OsMADS1 promoter in rice Oryza sativa L.., These findings demonstrated that WG7 upregulated OsMADS1 expression by directly binding to its promoter, enhanced histone H3K4me3<U+00A0>enrichment in the promoter and ultimately increased grain width
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS13, Transcriptome analysis reveals rice OsMADS13 as an important repressor of the carpel development pathway in ovules, Among a total of 476 Differentially Expressed Genes (DEGs), a substantial overlap with DEGs from the SEP-family osmads1 mutant was found, suggesting that OsMADS1 and OsMADS13 may act on a common set of target genes
  • CFO1~OsMADS32, OsLG3b~OsMADS1~LHS1~AFO, OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, Furthermore, our results indicate important roles of OsMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3 and OsMADS58 respectively
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS6~MFO1~AFG1, OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, Furthermore, our results indicate important roles of OsMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3 and OsMADS58 respectively
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS13, OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, Furthermore, our results indicate important roles of OsMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3 and OsMADS58 respectively
  • OsLG3b~OsMADS1~LHS1~AFO, OSMADS3, OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, Furthermore, our results indicate important roles of OsMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3 and OsMADS58 respectively
  • OsLG3b~OsMADS1~LHS1~AFO, OSMADS58, OsMADS32 Regulates Rice Floral Patterning through Interactions with Multiple Floral Homeotic Genes, Furthermore, our results indicate important roles of OsMADS32 in defining stamen, pistil and ovule development through physical and genetic interactions with OsMADS1, OsMADS58 and OsMADS13, and in specifying floral meristem identity with OsMADS6, OsMADS3 and OsMADS58 respectively
  • CFO1~OsMADS32, OsLG3b~OsMADS1~LHS1~AFO, Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • OsIG1, OsLG3b~OsMADS1~LHS1~AFO, Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS13, Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • OsLG3b~OsMADS1~LHS1~AFO, OsMADS15~DEP, Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • OsLG3b~OsMADS1~LHS1~AFO, OSMADS58, Characterization of a new rice OsMADS1 null mutant generated by homologous recombination-mediated gene targeting, Our data suggest, OsMADS1 commits and maintains determinate floret development by regulating floral meristem termination, carpel and ovule differentiation genes (OsMADS58, OsMADS13) while its modulation of genes such as OsMADS15, OsIG1 and OsMADS32 could be relevant in the differentiation and development of palea
  • FON2~FON4~TG1, OsLG3b~OsMADS1~LHS1~AFO, Interactions between FLORAL ORGAN NUMBER4 and floral homeotic genes in regulating rice flower development., Here, we report the genetic interactions between FON4 and floral homeotic genes OsMADS15 (an A-class gene), OsMADS16 (also called SUPERWOMAN1, SPW1, a B-class gene), OsMADS3 and OsMADS58 (C-class genes), OsMADS13 (a D-class gene), and OsMADS1 (an E-class gene) during flower development
  • FON2~FON4~TG1, OsLG3b~OsMADS1~LHS1~AFO, Interactions between FLORAL ORGAN NUMBER4 and floral homeotic genes in regulating rice flower development., Double mutant combinations of fon4 with osmads3, osmads58, osmads13, and osmads1 displayed enhanced defects in FM determinacy and identity, respectively, indicating that FON4 and these genes synergistically control FM activity
  • OsLG3b~OsMADS1~LHS1~AFO, OsPIN1c, Auxin Efflux Transporters OsPIN1c and OsPIN1d Function Redundantly in Regulating Rice Oryza sativa L. Panicle Development., Additionally, several critical regulators of reproductive development, such as OsPID, LAX1, OsMADS1, and OsSPL14/IPA1, were differentially expressed in ospin1c-1 ospin1d-1, supporting the hypothesis that OsPIN1c and OsPIN1d are involved in regulating panicle development
  • OsLG3b~OsMADS1~LHS1~AFO, OsPIN1d, Auxin Efflux Transporters OsPIN1c and OsPIN1d Function Redundantly in Regulating Rice Oryza sativa L. Panicle Development., Additionally, several critical regulators of reproductive development, such as OsPID, LAX1, OsMADS1, and OsSPL14/IPA1, were differentially expressed in ospin1c-1 ospin1d-1, supporting the hypothesis that OsPIN1c and OsPIN1d are involved in regulating panicle development
  • OsbZIP47, OsLG3b~OsMADS1~LHS1~AFO, OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development., Protein interaction assays showed OsbZIP47 partnerships with RICE HOMEOBOX1 (OSH1), RICE FLORICULA/LEAFY (RFL), and OsMADS1 transcription factors
  • OsbZIP47, OsLG3b~OsMADS1~LHS1~AFO, OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development., The meta-analysis of KD panicle transcriptomes in OsbZIP47KD, OsMADS1KD, and RFLKD transgenics, combined with global OSH1 binding sites divulge potential targets coregulated by OsbZIP47, OsMADS1, OSH1, and RFL

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