OsPPR676,OsATP4

• Symbol: OsPPR676,OsATP4
• MSU: LOC_Os03g11670
• RAPdb: Os03g0215900

• A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice, 2017, Sci Rep.
• The PPR-SMR protein ATP4 is required for editing the chloroplast rps8 mRNA in rice and maize, 2020, Plant Physiol.

Genbank accession number

• Disruption of OsPPR676 resulted in growth retardation of plants and partial sterility of pollens
• In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development
• Compared with other chloroplast-rich tissues, OsPPR676 was only weakly expressed in anther, but in the Mei and YM stages of pollen development, its expression was relatively strong in the tapetum
• osatp4 mutants were chlorotic and had a plastid-ribosome deficiency when grown in the cold
• Expression of the edited rps8 isoform in transgenic osatp4 mutants complemented the cold-sensitive phenotype, indicating that a rps8 expression defect accounts for the cold sensitivity

• OsBTF3~Osj10gBTF3, OsPPR676~OsATP4, A Plastid-Localized Pentatricopeptide Repeat Protein is Required for Both Pollen Development and Plant Growth in Rice, In this study, OsPPR676 was confirmed to be an interacted protein with Osj10gBTF3, β-subunit of nascent polypeptide-associated complex (β-NAC), by bimolecular fluorescence complementation assays, indicating that both proteins are probably involved in the same regulatory pathway of pollen development
• OsBTF3~Osj10gBTF3, OsPPR676~OsATP4, Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice, Knockout of Osj10gBTF3 affects pollen and chloroplast development and significantly reduces the accumulation of fertility-related chloroplast protein OsPPR676
• OsBTF3~Osj10gBTF3, OsPPR676~OsATP4, Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice, Both Osj10gBTF3 and OsHSP82 interact with OsPPR676
• OsBTF3~Osj10gBTF3, OsPPR676~OsATP4, Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice, Interestingly, the interaction between OsHSP82 and OsPPR676 is only found in the cytoplasm, while the interaction between Osj10gBTF3 and OsPPR676 also occurs inside the chloroplast
• OsBTF3~Osj10gBTF3, OsPPR676~OsATP4, Osj10gBTF3-Mediated Import of Chloroplast Protein Is Essential for Pollen Development in Rice, Further investigation indicates that Osj10gBTF3 enters the chloroplast stroma possibly through the inner chloroplast membrane channel protein Tic110 and then recruits OsCpn60 for the folding or assembly of OsPPR676