| Categories genes  | Tags chloroplast  phosphate  ja  JA  defense  tolerance  defense response  immune response  xoo  sa  SA  drought  abiotic stress  stress  biotic stress  abscisic acid  chlorophyll content  drought stress  water loss  drought stress  stress response  drought stress response 
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    • A self-ubiquitination assay indicated that the bacterially expressed OsUPS protein had E3 ligase activity, and subcellular localization results showed that OsUPS was located in the chloroplast
    • Here, we present the isolation of a novel U-box protein, U-box containing E3 ligase induced by phosphate starvation (OsUPS), from rice (Oryza sativa)
    • We found that full-length expression of OsUPS was up-regulated in both rice plants and cell culture in the absence of inorganic phosphate (P(i))
    • Molecular cloning and characterization of OsUPS, a U-box containing E3 ligase gene that respond to phosphate starvation in rice (Oryza sativa)
    • In rice, transient overexpression of OsPUB41 leads to enhanced expression of PR genes and SA as well as JA biosynthetic and response genes
    • However, in Arabidopsis, ectopic expression of OsPUB41 results in upregulation of only JA biosynthetic and response genes
    • This indicates that the E3 ligase activity of OsPUB41 protein is essential for induction of plant defense responses
    • Overexpression of OsPUB41 leads to induction of callose deposition, enhanced tolerance to Xoo and Rhizoctonia solani infection in rice and Arabidopsis respectively
    • Transient overexpression of either of the two biochemically inactive mutants (OsPUB41C40A and OsPUB41V51R) of OsPUB41 in rice and stable transgenics in Arabidopsis ectopically expressing OsPUB41C40A failed to elicit immune responses
    • The results presented here suggest that OsPUB41 is possibly involved in elicitation of CWDE triggered immune responses in rice
    • OsPUB41 plays a negative role in drought stress response through the mediation of OsUBC25 and interacts with OsCLC6, suggesting a putative substrate
    • The Ubi:RNAi-OsPUB41 knock-down and ospub41 suppression mutant plants exhibited enhanced tolerance to drought stress compared with the wild-type rice plants in terms of transpirational water loss, long-term dehydration response, and chlorophyll content
    • OsPUB41 expression was specifically induced by dehydration among various abiotic stresses and abscisic acid (ABA) treatments
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