| Categories genes  | Tags pollen  anther  sterility  development  starch  vegetative  breeding  tapetal  male sterility  pollen development 
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    • We then developed osdgd2β mutants by using the CRISPR/Cas9 system and elucidate its role, especially in the development of anther and pollen
    • Overall, we showed the importance of DGDG in pollen development and loss of function of OsDGD2β results in male sterility
    • ) genome has 5 genes encoding DGDG synthase, which are differentially expressed in different tissues, and OsDGD2β was identified to be the sole DGDG synthase gene expressed in anther
    • The mutants had no notable differences in the vegetative phenotype, as corroborated by relative gene expression of DGDG synthase genes in leaves, chlorophyll measurements, and analysis of photosynthetic parameters, implying the specificity of OsDGD2β in anther
    • OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice.
    • The loss of function of OsDGD2β resulted in male sterility in rice characterized by pale yellow and shrunken anther, devoid of starch granules in pollen, and delayed degeneration of tapetal cells
    • Here, we have also proposed the use of OsDGD2β in hybrid rice breeding using the nuclear male sterility system
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