OsAPX1,APXa

2015-01-20 | Categories genes  | Tags cold stress  growth  chilling  seedling  temperature  transcription factor  seed  seed germination  zinc  transcriptional activator  resistance  salicylic acid  blast  immunity  magnaporthe oryzae  innate immunity  homeostasis  blast resistance  transcription regulator  phytohormone 

• Information
  • Symbol: OsAPX1,APXa
  • MSU: LOC_Os03g17690
  • RAPdb: Os03g0285700
• Publication
  • Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings, 2012, J Plant Physiol.
  • Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling, 2003, Gene.
  • Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa, 2011, Plant Cell Rep.
  • Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings, 2001, J Exp Bot.
  • Purification and characterization of two ascorbate peroxidases of rice Oryza sativa L. expressed in Escherichia coli, 2005, Biotechnol Lett.
  • The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., 2017, Biochem Biophys Res Commun.
  • OsAPX1 Positively Contributes to Rice Blast Resistance., 2022, Front Plant Sci.
  • OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice., 2022, Plant J.
• Genbank accession number
  • AK061841
  • D45423
  • AK061841
  • D45423
• Key message
  • The APXa mRNA level in preheated seedlings was still higher than unheated seedlings under cold stress
  • Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling
  • To determine whether antioxidant capacity affects the ability of rice plants to withstand chilling at the booting stage, we produced transgenic rice plants that overexpress OsAPXa and have increased APX activity
  • Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa
  • APXa mRNA levels increased within 1 h after seedlings were exposed to 42 °C
  • Heat shock induction of the APXa gene could be a possible cause of reduced chilling injury in rice seedlings
  • The cytosolic APX gene expression in response to high and low temperature was analysed with an APXa gene probe
  • The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • Here, by ChIP (chromatin immunoprecipitation)-seq, we found that ZFP36, a rice zinc finger transcription factor, could directly bind to the promoter of OsAPX1, coding an ascorbate peroxidase (APX) which has the most affinity for H2O2 (substrate; a type of ROS), and act as a transcriptional activator of OsAPX1 promoter
  • Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • OsAPX1 also affects salicylic acid (SA) synthesis and signaling, which contribute to blast resistance
  • OsAPX1 Positively Contributes to Rice Blast Resistance.
  • In conclusion, we show that OsAPX1 is a key factor that connects the upstream gene silencing and transcription regulatory routes with the downstream phytohormone and redox pathway, which provides an insight into the sophisticated regulatory network of rice innate immunity
  • Here, we report that OsAPX1 also promotes ROS production such that a delicate cellular ROS homeostasis is achieved temporally after Magnaporthe oryzae infection
• Connection
  • OsAPX1~APXa, OsAPX2~APXb, Purification and characterization of two ascorbate peroxidases of rice Oryza sativa L. expressed in Escherichia coli, Here, we describe an Escherichia coli expression system for the rapid and economic expression of two rice APX genes, APXa and APXb (GeneBank accession Nos
  • OsAPX1~APXa, OsAPX2~APXb, Purification and characterization of two ascorbate peroxidases of rice Oryza sativa L. expressed in Escherichia coli, The GST-APXa and GST-APXb fusion proteins were purified by affinity chromatography using a glutathione-Sepharose 4B column, with final yields of 40 and 73 mg g(-1) dry cells, respectively
  • BSR-D1~ZFP36, OsAPX1~APXa, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • BSR-D1~ZFP36, OsAPX1~APXa, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Here, by ChIP (chromatin immunoprecipitation)-seq, we found that ZFP36, a rice zinc finger transcription factor, could directly bind to the promoter of OsAPX1, coding an ascorbate peroxidase (APX) which has the most affinity for H2O2 (substrate; a type of ROS), and act as a transcriptional activator of OsAPX1 promoter
  • BSR-D1~ZFP36, OsAPX1~APXa, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Moreover, ZFP36 could interact with a late embryogenesis abundant protein OsLEA5 to co-regulate the promoter activity of OsAPX1
  • BSR-D1~ZFP36, OsAPX1~APXa, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice.
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Moreover, ZFP36 could interact with a late embryogenesis abundant protein OsLEA5 to co-regulate the promoter activity of OsAPX1
  • OsAPX1~APXa, OsLEA5, The ascorbate peroxidase APX1 is a direct target of a zinc finger transcription factor ZFP36 and a late embryogenesis abundant protein OsLEA5 interacts with ZFP36 to co-regulate OsAPX1 in seed germination in rice., Thus, our conclusion is that OsAPX1 is a direct target of ZFP36 and OsLEA5 could interact with ZFP36 to co-regulate ABA-inhibited seed germination by controlling the expression of OsAPX1
  • OsAPX1~APXa, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., OsLEA5 acts as a co-regulator of a transcriptional factor ZFP36 to enhance the expression and the activity of ascorbate peroxidase OsAPX1 to regulate seed germination in rice, but it it unknown whether OsLEA5 is also crucial in plant seedlings under stress conditions
  • OsAPX1~APXa, OsLEA5, An Atypical Late Embryogenesis Abundant Protein OsLEA5 Plays A Positive Role In ABA-Induced Antioxidant Defense In Oryza Sativa L., Moreover, we found two homologue of OsLEA5 (5C700, short for Os05g0526700 and 5C300, short for Os05g0584300) which were induced by ABA also interacted with ZFP36 separately; interestingly, the nucleus-located 5C700 could also act as a co-activator of ZFP36 to modulate OsAPX1 while 5C300 which was ABA-induced downexpressed played as an ABA-induced inhibitor of ZFP36 to regulate OsAPX1
  • OsAPX1~APXa, OsEBP-89~OsEBP89, An APETALA2/ethylene responsive factor, OsEBP89 knockout enhances adaptation to direct-seeding on wet land and tolerance to drought stress in rice., Transcriptome data indicates that knockout of OsEBP89 improved the expression of specific genes in response to adverse factors, such as OsAPX1, OsHsfA3, and OsP5CS
  • OsAPX1~APXa, OsPQT3, Loss of Rice PARAQUAT TOLERANCE 3 Confers Enhanced Resistance to Abiotic Stresses and Increases Grain Yield in Field , The OsPQT3 knockout mutants (ospqt3) display enhanced resistance to oxidative and salt stress with elevated expression of OsGPX1, OsAPX1, and OsSOD1
  • OsAPX1~APXa, SodCc7~OsSOD1, Loss of Rice PARAQUAT TOLERANCE 3 Confers Enhanced Resistance to Abiotic Stresses and Increases Grain Yield in Field, The OsPQT3 knockout mutants (ospqt3) display enhanced resistance to oxidative and salt stress with elevated expression of OsGPX1, OsAPX1, and OsSOD1
  • OsABF1~OsABI5~OREB1~OsbZIP10, OsAPX1~APXa, OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice., Complementation assays suggested that the restored expression of OsSGT1 could not rescue the weak germination exhibitions of sgt1-1abi5 under Cr stress, even for those of Mel priming, but the expression of OsABI5, driven by the promoter of OsSGT1, significantly restored the Mel-ameliorated germination and the expression of ascorbate peroxidase 1 (OsAPX1) in sgt1-1abi5
  • OsABF1~OsABI5~OREB1~OsbZIP10, OsAPX1~APXa, OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice., Further analysis indicated that OsABI5 directly regulated the transcriptional expression of OsAPX1, whose encoding products promoted H(2) O(2) scavenging to maintain redox homeostasis, which was essential for germination
  • OsABF1~OsABI5~OREB1~OsbZIP10, OsAPX1~APXa, OsSGT1 promotes melatonin-ameliorated seed tolerance to chromium stress by affecting the OsABI5-OsAPX1 transcriptional module in rice., Collectively, this work demonstrates that OsSGT1 coordinates OsABI5 to target OsAPX1 conferring the melatonin-ameliorated germination of Cr-stressed seeds, which provides a guide for the applications of Mel in production

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