OsNAP

• Symbol: OsNAP
• MSU: LOC_Os03g21060
• RAPdb: Os03g0327800

• The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway, 2014, Plant Cell Physiol.
• Identification and functional characterization of a rice NAC gene involved in the regulation of leaf senescence, 2013, BMC Plant Biol.
• OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice, 2014, Proceedings of the National Academy of Sciences.

Genbank accession number

• OsNAP-overexpressing transgenic plants displayed an accelerated leaf senescence phenotype at the grain-filling stage, which might be caused by the elevated JA levels and the increased expression of the JA biosynthesis-related genes LOX2 and AOC1, and showed enhanced tolerance ability to MeJA treatment at the seedling stage
• Analysis of the OsNAP transcript levels in rice showed that this gene was significantly induced by ABA and abiotic stresses, including high salinity, drought and low temperature
• The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway
• RESULTS: In this study, we reported a member of the NAC transcription factor family named OsNAP whose expression is associated with leaf senescence, and investigated its preliminary function during the process of leaf senescence
• The results of qRT-PCR showed that the OsNAP transcripts were accumulated gradually in response to leaf senescence and treatment with methyl jasmonic acid (MeJA)
• Nevertheless, the leaf senescence process was delayed in OsNAP RNAi transgenic plants with a dramatic drop in JA levels and with decreased expression levels of the JA biosynthesis-related genes AOS2, AOC1 and OPR7
• CONCLUSIONS: These results suggest that OsNAP acts as a positive regulator of leaf senescence and this regulation may occur via the JA pathway
• Rice plants overexpressing OsNAP did not show growth retardation, but showed a significantly reduced rate of water loss, enhanced tolerance to high salinity, drought and low temperature at the vegetative stage, and improved yield under drought stress at the flowering stage
• Our data suggest that OsNAP functions as a transcriptional activator that plays a role in mediating abiotic stress responses in rice
• OsNAP is a member of the NAC transcription factor family; it is localized in the nucleus, and shows transcriptional activator activity in yeast
• Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59)
• OsNAP connects abscisic acid and leaf senescence by fine-tuning abscisic acid biosynthesis and directly targeting senescence-associated genes in rice
• Additionally, reduced OsNAP expression leads to delayed leaf senescence and an extended grain-filling period, resulting in a 6.3% and 10.3% increase in the grain yield of two independent representative RNAi lines, respectively.
• Thus, fine-tuning OsNAP expression should be a useful strategy for improving rice yield in the future

• OsABI2~OsPP2C06~OsABIL1, OsNAP, The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway, Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59)
• OsNAP, OsSIPP2C1~OsPP2C68~OsPP108, The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway, Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59)
• OsNAP, OsPP2C09~OsPP15~OsPYL2, The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway, Microarray analysis of transgenic plants overexpressing OsNAP revealed that many stress-related genes were up-regulated, including OsPP2C06/OsABI2, OsPP2C09, OsPP2C68 and OsSalT, and some genes coding for stress-related transcription factors (OsDREB1A, OsMYB2, OsAP37 and OsAP59)
• OsMYB102, OsNAP, The rice transcription factor OsMYB102 delays leaf senescence by downregulating abscisic acid accumulation and signaling., Further studies demonstrated that overexpression of OsMYB102 regulates the expression of SAGs, including genes associated with ABA degradation and ABA signaling (OsABF4, OsNAP, and OsCYP707A6), under dark-induced senescence conditions
• OsMYB102, OsNAP, The rice transcription factor OsMYB102 delays leaf senescence by downregulating abscisic acid accumulation and signaling., OsMYB102 also indirectly represses ABA-responsive genes, such as OsABF4 and OsNAP
• OsNAP, OsWRKY5, OsWRKY5 Promotes Rice Leaf Senescence via Senescence-Associated NAC and Abscisic Acid Biosynthesis Pathway., Furthermore, RT-qPCR and yeast one-hybrid analysis demonstrated that OsWRKY5 indirectly upregulates the expression of senescence-associated NAM/ATAF1/2/CUC2 (NAC) genes including OsNAP and OsNAC2
• OsNAP, OsRap2.6~OsERF101, Rice ETHYLENE RESPONSE FACTOR 101 Promotes Leaf Senescence Through Jasmonic Acid-Mediated Regulation of OsNAP and OsMYC2, Consistent with its delayed-yellowing phenotype, the oserf101 mutant exhibited downregulation of genes involved in chlorophyll degradation, including rice NAM, ATAF1/2, and CUC2 (OsNAP), STAY-GREEN (SGR), NON-YELLOW COLORING 1 (NYC1), and NYC3 during DIS
• OsNAP, OsRap2.6~OsERF101, Rice ETHYLENE RESPONSE FACTOR 101 Promotes Leaf Senescence Through Jasmonic Acid-Mediated Regulation of OsNAP and OsMYC2, Transient transactivation and chromatin immunoprecipitation assays revealed that OsERF101 directly binds to the promoter regions of OsNAP and OsMYC2, which activate genes involved in chlorophyll degradation and JA signaling-mediated leaf senescence
• OsNAC109, OsNAP, OsNAC109 regulates senescence, growth and development by altering the expression of and phytohormone-associated genes in rice., It was confirmed that OsNAC109 could directly regulate the expression of OsNAP, OsNYC3, OsEATB, OsAMTR1, OsZFP185, OsMPS and OsGA2ox3 by targeting to the highly conserved cis-element CNTCSSNNSCAVG except OsSAMS1