OsSRFP1,SDEL2

• Symbol: OsSRFP1,SDEL2
• MSU: LOC_Os03g22680
• RAPdb: Os03g0348900

• Knock-down of stress inducible OsSRFP1 encoding an E3 ubiquitin ligase with transcriptional activation activity confers abiotic stress tolerance through enhancing antioxidant protection in rice., 2015, Plant Mol Biol.
• Two RING-finger ubiquitin E3 ligases regulate the degradation of SPX4, the internal phosphate sensor, for phosphate homeostasis and signaling in rice., 2019, Mol Plant.
• OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , 2021, Rice (N Y).

Genbank accession number

• Interestingly, the OsSRFP1-overexpressing plants were less tolerant to salt, cold and oxidative stresses than wild type plants; while the RNA interference silencing of OsSRFP1 plants were more tolerant than wild type without yield penalty
• Knock-down of stress inducible OsSRFP1 encoding an E3 ubiquitin ligase with transcriptional activation activity confers abiotic stress tolerance through enhancing antioxidant protection in rice.
• Taken together, our results demonstrate that OsSRFP1 may have dual functions in post-translational and transcriptional regulations in modulating abiotic stress responses in rice, at least in part, by negatively regulating antioxidant enzymes-mediated reactive oxygen species removal
• OsSRFP1 was ubiquitously expressed in various rice organs, with the higher expression levels in roots, panicles and culm nodes
• The transcript of OsSRFP1 was induced by cold, dehydration, salt, H2O2 and abscisic acid treatments
• In vitro ubiquitination assays showed that OsSRFP1 possessed E3 ubiquitin ligase activity and the intact RING domain was essential for the activity
• Consistent with this biochemical function of SDEL1 and SDEL2, enhancing the expression of SDEL1 and SDEL2 results in Pi over-accumulation and induces Pi-starvation signaling even under Pi-sufficient conditions
• Therefore SDEL1 and SDEL2 facilitate the degradation of SPX4 to modulate PHR2 activity and regulate Pi-homeostasis and Pi-signaling in response to external Pi availability in rice

• OsMYB30~OsMYB5P, OsSRFP1~SDEL2, OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , Under cold stress treatments, the transcription of four cold-related genes OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT)
• OsMYB30~OsMYB5P, OsSRFP1~SDEL2, OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45-1 at the Seedling Stage in Rice , It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45-1, OsSRFP1, OsCYL4 and OsMYB30