- Information
- Symbol: OsIRO3,OsOsbHLH063
- MSU: LOC_Os03g26210
- RAPdb: Os03g0379300
- Publication
- Identification of a novel iron regulated basic helix-loop-helix protein involved in Fe homeostasis in Oryza sativa, 2010, BMC Plant Biol.
- OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice, 2020, Plants (Basel).
- A Model to Incorporate the bHLH Transcription Factor OsIRO3 within the Rice Iron Homeostasis Regulatory Network., 2022, Int J Mol Sci.
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., 2022, Plant J.
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Genbank accession number
- Key message
- RESULTS: Transcript abundance of one nuclear located basic helix-loop-helix family transcription factor, OsIRO3, is up-regulated from 25- to 90-fold under Fe deficiency in both root and shoot respectively
- CONCLUSION: A novel Fe regulated bHLH transcription factor (OsIRO3) that plays an important role for Fe homeostasis in rice was identified
- Furthermore, the Fe concentration in shoots of transgenic rice plants over-expressing OsIRO3 was less than that in wild-type plants
- OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage
- Knockout of OsIRO3 resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development
- OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice
- However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear
- Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice
- Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression
- A Model to Incorporate the bHLH Transcription Factor OsIRO3 within the Rice Iron Homeostasis Regulatory Network.
- We place our results in the context of the existing literature and generate a model describing the role of OsIRO3 in rice Fe homeostasis and reinforce the essential function of OsIRO3 in the rice Fe deficiency response
- Further analysis revealed that OsIRO3 directly regulated the expression of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2), which encodes a positive regulator of the Fe uptake system
- Loss-of-function of OsIRO3 caused upregulation of Fe deficiency-associated genes in the root
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.
- Further investigation demonstrated that OsIRO3 interacted with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1(OsPRI1) and OsPRI2, and
- This work sheds light on the molecular mechanism by which OsIRO3 modulates Fe homeostasis in rice
- OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2
- Connection
- OsIRO3~OsOsbHLH063, OsPRI1~OsbHLH115, POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 OsPRI1 positively regulates iron homeostasis in rice., Yeast one-hybrid and electrophoretic mobility shift assay results suggested that OsPRI1 binds to the OsIRO2 and OsIRO3 promoters
- OsIRO3~OsOsbHLH063, OsPRI1~OsbHLH115, POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 OsPRI1 positively regulates iron homeostasis in rice., Collectively, these results imply that OsPRI1, which is ubiquitinated by OsHRZ1, mediates rice responses to Fe-deficiency by positively regulating OsIRO2 and OsIRO3 expression as part of the OsHRZ1-OsPRI1-OsIRO2/3 signal transduction cascade
- OsbHLH59~OsbHLH059~OsPRI3, OsIRO3~OsOsbHLH063, Oryza sativa POSITIVE REGULATOR OF IRON DEFICIENCY RESPONSE 2 OsPRI2 and OsPRI3 are involved in the maintenance of Fe homeostasis., We also provide evidence that OsPRI2 and OsPRI3 bind to the promoters of OsIRO2 and OsIRO3, two key regulators of Fe homeostasis
- OsIRO3~OsOsbHLH063, OsNAS3, OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice, Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of OsNAS3
- OsIRO3~OsOsbHLH063, OsNAS3, OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice, Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2.
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., Further analysis revealed that OsIRO3 directly regulated the expression of IRON-RELATED BHLH TRANSCRIPTION FACTOR 2 (OsIRO2), which encodes a positive regulator of the Fe uptake system
- OsIRO2~OsbHLH056, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., OsIRO3 repressed their transcription activation towards OsIRO2
- OsbHLH058~OsPRI2, OsIRO3~OsOsbHLH063, OsIRO3 negatively regulates Fe homeostasis by repressing the expression of OsIRO2., Further investigation demonstrated that OsIRO3 interacted with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1(OsPRI1) and OsPRI2, and
- OsbHLH061~OsbHLHq11, OsIRO3~OsOsbHLH063, OsbHLH061 links TOPLESS/TOPLESS-RELATED repressor proteins with POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 to maintain iron homeostasis in rice., Mechanistically, OsbHLH061 expression is upregulated by high Fe, and physically interacts with OsPRI1, the OsbHLH061-OsPRI1 complex recruits TOPLESS/TOPLESS-RELATED (OsTPL/TPR) co-repressors to repress OsIRO2 and OsIRO3 expression
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